PHYTOCHEMICAL STUDY, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF<i>Stemodia maritima</i>

Silva, Francisca R. L. da; Rodrigues, Francisco E. A.; Gomes, Aldenia R. S.; Arriaga, Ângela M. C.; Mafezoli, Jair; Lemos, Telma L. G.; Almeida, Macia C. S. de; Santiago, Gilvandete Maria Pinheiro; Braz-Filho, Raimundo; Costa, José G. M.; Rodrigues, F. R. N.; Coutinho, Henrique Douglas Melo

doi:10.5935/0100-4042.20140255

Cited by 6 publications

(16 citation statements)

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“…This study observed that 5 mg/kg S. maritima extract reduced alveolar bone loss, inflammation, and oxidative stress in a ligature-induced model of periodontitis in rats without causing any systemic changes. These data are in accordance with previous results from our group, which demonstrated via in vitro assays that S. maritima has anti-inflammatory, antioxidant and antibacterial properties (da Silva et al, 2014 ). We also found evidence that, at least in part, the effectiveness of S. maritima depends upon a positive balance between pro and anti-inflammatory cytokines that decrease TNF-α, IL-1β, and CINC-1 gingival levels while increasing IL-10.…”

Section: Discussionsupporting

confidence: 94%

“…Further, it was showed that S. maritima compounds have in vitro inhibitory activity of both lipids peroxidation and cyclooxygenase 1 and 2 (Hufford et al, 1992 ; Russell et al, 2011 ). Besides, our research group demonstrated that S. maritima has antioxidant activity in vitro and it also has activity against some bacterial strains (da Silva et al, 2014 ). However, it is important to mention that there is no study in the literature using S. maritima extract or its identified compounds on bone resorption models.…”

Section: Discussionmentioning

confidence: 99%

“…Dr. F. S. Cavalcanti and Prof. E. P. Nunes identified the plant and a voucher specimen (# 38483) was registered at Prisco Bezerra Herbarium, Federal University of Ceará, Fortaleza, Brazil. For this study, the extract was obtained from fresh leaves of Sm collected during the flowering stage along Fleixeiras Beach, Ceará, Brazil (da Silva et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%

“…In this regard, earlier chemical studies on Stemodia maritima L. (Plantaginaceae family, formely Scrophulariaceae), which is used in traditional medicine to treat inflammatory disorders, reported the isolation of flavonoids, diterpenes, and other compounds associated with larvicidal and antiviral activities (Rodrigues et al, 2010 ; Russell et al, 2011 ). Our group studied the phytochemicals in S. maritima L., and described the isolation of the natural compound, stemodinol along with seven known compounds (da Silva et al, 2014 ). Antioxidant and antimicrobial activities were observed on crenatoside, stemodinoside B, and stemodin.…”

Section: Introductionmentioning

confidence: 99%

“…Antioxidant and antimicrobial activities were observed on crenatoside, stemodinoside B, and stemodin. These are natural compounds also derived from S. maritima L. (da Silva et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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Stemodia maritima L. Extract Decreases Inflammation, Oxidative Stress, and Alveolar Bone Loss in an Experimental Periodontitis Rat Model

et al. 2017

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Periodontitis is very prevalent worldwide and is one of the major causes of tooth loss in adults. About 80% of the worldwide population use medicinal plants for their health care. Stemodia maritima L. (S. maritima) antioxidant and antimicrobial effects in vitro as well as anti-inflammatory properties. Herein, the potential therapeutic effect of S. maritima was assessed in rats subjected to experimental periodontitis (EP). EP was induced in female Wistar rats by nylon thread ligature around 2nd upper left molars for 11 days. Animals received (per os) S. maritima (0.2; 1 or 5 mg/kg) or vehicle (saline + DMSO) 1 h before ligature and then once daily for 11 days. The naive group had no manipulation. After this time-point, the animals were terminally anesthetized, and the maxillae were removed for morphometric and histological analyzes (HE). Gingival tissues were dissected to cytokine levels detection (TNF-α, IL1-β, CINC-1, and IL-10), enzymes superoxide dismutase (SOD), and catalase (CAT) analysis, as well as gene expression (TNF-α, IL-1β, RANK, and iNOS) by qRT-PCR. Systemic parameters (weight variation, plasma levels of hepatic enzymes aspartate aminotransferase (AST) and alanine aminotransferase (ALT), creatinine, total alkaline phosphatase (TALP), and bone alkaline phosphatase (BALP) were performed. Histological analysis of the stomach, liver, kidney, and heart was also performed. S. maritima (5 mg/kg) decreased alveolar bone loss, TNF-α and CINC-1 gingival levels, oxidative stress, and transcription of TNF-α, IL1-β, RANK, and iNOS genes. It elevated both BALP activity and IL-10 gingival levels. The animals showed no any signs of toxicity. In conclusion, S. maritima reduced pro-inflammatory cytokine production, oxidative stress, and alveolar bone loss in a pre-clinical trial of periodontitis. S. maritima is a potential tool for controlling the development of periodontitis.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Antioxidant and antimicrobial activities were observed on crenatoside, stemodinoside B, and stemodin. These are natural compounds also derived from S. maritima L. (da Silva et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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Stemodia maritima L. Extract Decreases Inflammation, Oxidative Stress, and Alveolar Bone Loss in an Experimental Periodontitis Rat Model

et al. 2017

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Antioxidant and gastroprotective effects of the ethyl acetate extract from Stemodia maritima L. in ethanol-induced gastric ulcer model

Sousa

Silva

Borba

et al. 2021

RSD

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Stemodia maritima L., is a shrub of the Plantaginaceae family, with some biological activities already described, such as: larvicide, antimicrobial, and anti-inflammatory. Thus, the objective of this work was to evaluate the antioxidant, and gastroprotective activities of the ethyl acetate extract from S. maritima. The phytochemical profile was investigated through the quantification of total phenolic compounds, flavonoids, and CCD analysis. The toxicity of the extract was performed through cell viability using L929 line cell, and acute toxicity by the OECD Guide 423. The antioxidant activity was analyzed by the methods of reduction of the ferric ion (FRAP), total antioxidant activity (TAA), and the gastroprotective activity by the absolute ethanol-induced gastric ulcer model, with analysis of NO, MDA, GSH and MPO levels in the stomach tissues. In the phytochemical profile it was possible to identify the presence of flavonoids, triterpenes, steroids, mono, and sesquiterpenes. The extract was not cytotoxic against L929 lineage, maintaining cell viability above 70% at the doses tested, and in acute toxicity it did not show physiological changes indicative of toxicity compared to the control group. The extract presented antioxidant activity of 157.3 ± 9.7 mg equivalent of Trolox/g of extract in the FRAP method, and 50.0 ± 1.1 % by TAA. The ethyl acetate extract of S. maritima, at the doses tested, reduced the ulcerative lesion index compared to the injured control group, increased the levels of NO and GSH, and was able to decrease the concentrations of MDA and MPO, enhancing their gastroprotective activity.

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Orobanche crenata Forssk. Extract Affects Human Breast Cancer Cell MCF-7 Survival and Viral Replication

Genovese

Garozzo

D’Angeli

et al. 2022

Cells

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Background: Breast cancer (BC) is the leading cause of death worldwide. The severity of BC strictly depends on the molecular subtype. The less aggressive hormone-positive subtype is treated with adjuvant endocrine therapy (AET), which causes both physical and psychological side effects. This condition strongly impacts the adherence and persistence of AET among oncologic patients. Moreover, viral infections also constitute a serious problem for public health. Despite their efficacy, antiviral agents present several therapeutic limits. Accordingly, in the present work, we investigated the antitumor and antiviral activities of Orobanche crenata Forssk. (O. crenata), a parasitic plant, endemic to the Mediterranean basin, traditionally known for its beneficial properties for human health. Methods: The MTT assay was carried out to evaluate the cytotoxic effect of O. crenata leaf extract (OCLE) on human breast cancer cells (MCF-7 and MDA-MB-231) and the primary HFF-1 cell line. The lactic dehydrogenase (LDH) assay was performed on MCF-7 cells to analyze necrotic cell death. The antioxidant effect of OCLE was evaluated by intracellular determination of the reactive oxygen species and thiol groups, by DPPH and ABTS assays. The antiviral activity of OCLE was determined against Poliovirus 1, Echovirus 9, Human respiratory syncytial virus, Adenovirus type 2 and type 5, Coxsackievirus B1 (CoxB1) and B3 (CoxB3), Herpes simplex type 1 (HSV-1) and type 2 (HSV-2), and β-Coronavirus by the plaque reduction assay. Results: The extract, after 24 h of incubation, did not affect MDA-MB-231 and HFF-1 cell viability. However, at the same time point, it showed a dose-dependent inhibitory effect on MCF-7 cells, with an increase in LDH release. OCLE exhibited free radical scavenging activity and significantly increased non-protein thiol levels in MCF-7 cells. OCLE effectively inhibited HSV-1, HSV-2, CoxB1, and CoxB3 replication. Conclusions: The overall results showed an interesting inhibitory effect of OCLE on both MCF-7 cell survival and viral replication.

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PHYTOCHEMICAL STUDY, ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OFStemodia maritima

Cited by 6 publications

References 0 publications

Stemodia maritima L. Extract Decreases Inflammation, Oxidative Stress, and Alveolar Bone Loss in an Experimental Periodontitis Rat Model

Stemodia maritima L. Extract Decreases Inflammation, Oxidative Stress, and Alveolar Bone Loss in an Experimental Periodontitis Rat Model

Antioxidant and gastroprotective effects of the ethyl acetate extract from Stemodia maritima L. in ethanol-induced gastric ulcer model

Orobanche crenata Forssk. Extract Affects Human Breast Cancer Cell MCF-7 Survival and Viral Replication

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