Induction of corneal collagen cross-linking in experimental corneal alkali burns in rabbits

Colombo-Barboza, Marcello Novoa; Barboza, Guilherme Novoa Colombo; Felberg, Sérgio; Dantas, Paulo E.C.; Sato, Élcio Hideo

doi:10.5935/0004-2749.20140078

Cited by 13 publications

(5 citation statements)

References 14 publications

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“…The experimental lesion was induced with a chemical technique to obtain an alkali-induced corneal stromal melting, as reported in rabbits [ 31 , 32 , 33 , 34 ]. The induced lesions on the cornea were produced using a filter paper (0.8 cm) soaked with NaOH (1N) that caused significant tissue damage on contact.…”

Section: Methodsmentioning

confidence: 99%

An Assay System to Evaluate Riboflavin/UV-A Corneal Phototherapy Efficacy in a Porcine Corneal Organ Culture Model

Perazzi

Gomiero

Corain

et al. 2020

Animals

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The purpose of this study was to investigate the response of porcine corneal organ cultures to riboflavin/UV-A phototherapy in the injury healing of induced lesions. A porcine corneal organ culture model was established. Corneal alterations in the stroma were evaluated using an assay system, based on an automated image analysis method able to (i) localize the holes and gaps within the stroma and (ii) measure the brightness values in these patches. The analysis has been performed by dividing the corneal section in 24 regions of interest (ROIs) and integrating the data analysis with a "multi-aspect approach." Three group of corneas were analyzed: healthy, injured, and injured-and-treated. Our study revealed a significant effect of the riboflavin/UV-A phototherapy in the injury healing of porcine corneas after induced lesions. The injured corneas had significant differences of brightness values in comparison to treated (p < 0.00) and healthy (p < 0.001) corneas, whereas the treated and healthy corneas showed no significant difference (p = 0.995). Riboflavin/UV-A phototherapy shows a significant effect in restoring the brightness values of damaged corneas to the values of healthy corneas, suggesting treatment restores the injury healing of corneas after lesions. Our assay system may be compared to clinical diagnostic methods, such as optical coherence tomography (OCT) imaging, for in vivo damaged ocular structure investigations.

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Section: Methodsmentioning

confidence: 99%

An Assay System to Evaluate Riboflavin/UV-A Corneal Phototherapy Efficacy in a Porcine Corneal Organ Culture Model

Perazzi

Gomiero

Corain

et al. 2020

Animals

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“…Chemical burns, which are usually induced by alkaline or acidic agents, are also a common cause of ophthalmic injuries. In addition, corneal alkali burn is considered the most dangerous ocular emergency that can result in permanent visual impairment . Surgical antibiotics, ascorbic acid, glucocorticoids, and amniotic membrane transplantation are currently applied interactively to treat corneal alkali burn .…”

Section: Introductionmentioning

confidence: 99%

Tetrahedral Framework Nucleic Acids Promote Corneal Epithelial Wound Healing in Vitro and in Vivo

Liu

Zhang

et al. 2019

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Poor post‐traumatic wound healing can affect the normal function of damaged tissues and organs. For example, poor healing of corneal epithelial injuries may lead to permanent visual impairment. It is of great importance to find a therapeutic way to promote wound closure. Tetrahedral framework nucleic acids (tFNAs) are new promising nanomaterials, which can affect the biological behavior of cells. In the experiment, corneal wound healing is used as an example to explore the effect of tFNAs on wound healing. Results show that the proliferation and migration of human corneal epithelial cells are enhanced by exposure to tFNAs in vitro, possibly relevant to the activation of P38 and ERK1/2 signaling pathway. An animal model of corneal alkali burn is established to further identify the facilitation effect of tFNAs on corneal wound healing in vivo. Clinical evaluations and histological analyses show that tFNAs can improve the corneal transparency and accelerate the re‐epithelialization of wounds. Both in vitro and in vivo experiments show that tFNAs can play a positive role in corneal epithelial wound healing.

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“…Riboflavin-ultraviolet-A corneal cross-linking (CXL) is an established treatment to enhance biomechanical stability, increase corneal resistance to enzymatic digestion, and inhibit corneal edema [ 3 – 5 ]. Moreover, in a corneal alkali burn model, epithelial defects are attenuated by CXL treatment, which suggests that CXL ameliorates the effects of acute corneal alkali burns [ 6 ]. In the present study, we investigated the pathogenic process of alkali burn-induced CNV with or without riboflavin-ultraviolet-A-induced cross-linking and measured the expression of VEGF, MMP-2, and MMP-9 to determine the molecular mechanism of CXL for promoting angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

Effect of Collagen Cross-Linking on Alkali Burn-Induced Corneal Neovascularization in Rabbits

Liu

2018

Journal of Ophthalmology

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Objective This study aims at investigating the effects and molecular mechanism of riboflavin-ultraviolet-A-induced cross-linking (corneal collagen cross-linking, CXL) on corneal neovascularization (CNV) in a rabbit alkali burn model. Methods A total of 60 rabbits were injured with alkali burns to induce CNV in the right eye and were randomly divided into six groups: Group A—injury and no treatment; Groups B, C, and D—CXL treatment for 30 min, 15 min, and 45 min administered immediately after injury, respectively; and Groups E and F—CXL treatment for 30 min administered 1 day and 3 days after injury, respectively. CNV area, corneal edema, and corneal epithelial defects were observed on days 4, 7, 10, and 14 after injury. Western blot was used to detect expression of the vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-2 (MMP-9), and tissue inhibitor of metalloproteinases 1 (TIMP-1) at 7 and 14 days after injury. Results CXL treatment decreased CNV and corneal edema in all groups compared to Group A. On day 7, MMP-9 expression was significantly increased in all CXL treatment groups, and TIMP-1 was upregulated in Groups D and F compared to Group A. In addition, VEGF, MMP-2, MMP-9, and TIMP-1 expression were increased in Group A on day 14 after injury. Conclusions Our results indicate that riboflavin-ultraviolet-A-induced cross-linking (corneal collagen cross-linking, CXL) significantly inhibits alkali burn-induced CNV in rabbits, possibly through downregulating VEGF, MMP-2, MMP-9, and TIMP-1 expression.

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Induction of corneal collagen cross-linking in experimental corneal alkali burns in rabbits

Cited by 13 publications

References 14 publications

An Assay System to Evaluate Riboflavin/UV-A Corneal Phototherapy Efficacy in a Porcine Corneal Organ Culture Model

An Assay System to Evaluate Riboflavin/UV-A Corneal Phototherapy Efficacy in a Porcine Corneal Organ Culture Model

Tetrahedral Framework Nucleic Acids Promote Corneal Epithelial Wound Healing in Vitro and in Vivo

Effect of Collagen Cross-Linking on Alkali Burn-Induced Corneal Neovascularization in Rabbits

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