Efficacy of a lutein-based dye (Phacodyne<sup>TM</sup>) for visualizing anterior capsulorhexis during cataract surgery by phacoemulsification

Vianna, Lucas Monferrari Monteiro; Cohen, Marcos Jacob; Muccioli, Cristina; Lima, Acácio; Sousa-Martins, Diogo; Maia, Maurício; Belfort, Rubens

doi:10.5935/0004-2749.20140044

Cited by 5 publications

(6 citation statements)

References 19 publications

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“…This combination was used during cataract surgery by phacoemulsification for screening anterior capsulorhexis in patients. The result of this study indicated that this combined dye might be an alternative for continuous circular capsulorhexis (CCC) in human due to its safe, effective and antioxidant profile [72]. The results of Chew et al's study was contrary to the most studies.…”

Section: Role Of Lutein and Zeaxanthin In Cataractcontrasting

confidence: 56%

An Overview on the Role of Macular Xanthophylls in Ocular Diseases

Sarıaltın¹,

Çoban²

2018

Rec.Nat.Prod.

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Macula lutea, is the center of the retina of the eye, contains high concentrations of lutein and zeaxanthin which can act as a filter against short-wavelength (blue) light. Lutein and zeaxanthin are the only carotenoids detected in human lens which exhibit highly strong free radical scavenging activity. Many epidemiological studies, clinical trials, animal experiments have suggested that lutein and zeaxanthin have antiinflammatory potential with their high antioxidant properties. Several eye diseases including, age-related macular degeneration, uveitis and retinitis pigmentosa are caused by ocular inflammation. Some studies have shown that lutein and zeaxanthin could be protective, curative and preventive against ocular inflammation induced diseases and other ocular disorders such as cataract, glaucoma and choroideremia. The mechanisms responsible for these effects are absorption of near-ultraviolet and blue light, reduction of oxidative stress, inflammation and angiogenesis. Lutein and zeaxanthin can be taken from dietary supplements or a diet high in fruits, vegetables such as kale, spinach and turnip greens. The aim of this review is to evaluate the relationship between the consumption of lutein and zeaxanthin and eye diseases.

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Section: Role Of Lutein and Zeaxanthin In Cataractcontrasting

confidence: 56%

An Overview on the Role of Macular Xanthophylls in Ocular Diseases

Sarıaltın¹,

Çoban²

2018

Rec.Nat.Prod.

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“…Previous reports had already shown the safety and efficacy of Phacodyne during cataract surgery. 27 Although these results indicate a similar behavior for these two dyes, other studies point to retinal toxicity of TB. Several studies have described retinal damage after exposure to TB in a bovine model, as well as, in vitro rodent neurosensory cells.…”

Section: Discussionmentioning

confidence: 78%

“…[19][20][21] In our previous publications, we have described the use of novel intraocular dyes in which Lutein is the primary component in cataract and vitreoretinal surgeries. [22][23][24][25][26][27][28] These L/Z-based dyes have shown efficacy in staining target membranes such as AC, ILM, and the vitreous, as well as an excellent safety profile. [22][23][24][25][26][27][28] The nature of the interaction of L/Z-based dyes with their target membranes is herein investigated using Langmuir monolayer models simulating the ILM, vitreous, AC, and epiretinal membrane (ERM).…”

mentioning

confidence: 99%

Comparing the Mode of Action of Intraocular Lutein-Based Dyes With Synthetic Dyes

Sousa-Martins

Caseli

Figueiredo

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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“…Lutein/trypan blue dye was tested as a good, useful, and safe dye during capsulorhexis in phacoemulsification. No signs of toxicity or side effects were detected during follow-up (30 days) [ 10 ]. This protective effect was associated with the properties of lutein, as suggested by clinical, histological, and electroretinographic analyses performed to evaluate the safety profile of solutions containing lutein in rabbit eyes after intravitreal injections, implying good protection of vitreoretinal interphase and underlined retina [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

The usefulness of lutein/trypan blue vital dye for the staining of corneal endothelium: a pilot study on DMEK pretreated tissues

Gisoldi

Lodato

Balzamino

et al. 2022

Graefes Arch Clin Exp Ophthalmol

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Purpose The study aims to evaluate the usefulness of lutein/trypan blue vital dye for the staining of corneal tissues and endothelium–Descemet membrane (EDM) for Descemet membrane endothelial keratoplasty (DMEK). Methods Sixteen human corneal tissues (Eye Bank, Rome, Italy) were used. Corneal endothelium was tested at 25 s (T0), 1 min (T1), 2 min (T2), and 4 min (T4) from dye addition. Staining intensity and cell counting were compared. Stripped EDM was analyzed for selected apoptotic (AP, caspases, BCL2, BAX) and differentiation (VEGF-A, TGF-β1RI, SMAD3/7, SMA) targets and changes in target expression. Protein extracts were analyzed through SDS-PAGE/IB. Results Although trypan blue staining produced the same color intensity of lutein/trypan blue dye in half the time, lutein/trypan blue reached a good and adequate color intensity at T4, which persisted even on excised and washed EDM grafts. Lutein/trypan blue-stained EDM showed a reduced number of blue-stained cells and AP immunoreactivity was significantly reduced in the same samples. An increased BCL2 transcript and a reduced BAX transcript were detected in lutein/trypan blue-stained EDM. No significant changes were observed for the main effector caspases (3/9) upon both treatments and the target genes representative of endothelial cell trans-differentiation (TGF-β1RI, SMAD3/7, SMA). A trend in vascular endothelial growth factor (VEGF-A) regulation was observed in lutein/trypan blue-treated EDM grafts. Conclusion Obtained results suggest that lutein/trypan blue dye deserves attention in the DMEK field and support the potential routine use of this dye as a valid alternative to trypan blue for all procedures devoted to the assessment of endothelial cell viability and visualization of EDM graft before DMEK grafting. Supplementary Information The online version contains supplementary material available at 10.1007/s00417-022-05909-x.

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Efficacy of a lutein-based dye (Phacodyne^TM) for visualizing anterior capsulorhexis during cataract surgery by phacoemulsification

Cited by 5 publications

References 19 publications

An Overview on the Role of Macular Xanthophylls in Ocular Diseases

An Overview on the Role of Macular Xanthophylls in Ocular Diseases

Comparing the Mode of Action of Intraocular Lutein-Based Dyes With Synthetic Dyes

The usefulness of lutein/trypan blue vital dye for the staining of corneal endothelium: a pilot study on DMEK pretreated tissues

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Efficacy of a lutein-based dye (PhacodyneTM) for visualizing anterior capsulorhexis during cataract surgery by phacoemulsification

Cited by 5 publications

References 19 publications

An Overview on the Role of Macular Xanthophylls in Ocular Diseases

An Overview on the Role of Macular Xanthophylls in Ocular Diseases

Comparing the Mode of Action of Intraocular Lutein-Based Dyes With Synthetic Dyes

The usefulness of lutein/trypan blue vital dye for the staining of corneal endothelium: a pilot study on DMEK pretreated tissues

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Efficacy of a lutein-based dye (Phacodyne^TM) for visualizing anterior capsulorhexis during cataract surgery by phacoemulsification