Protective effect of flavonoids against reactive oxygen species production in sickle cell anemia patients treated with hydroxyurea

Henneberg, Raílson; Otuki, Michel Fleith; Furman, Aline Emmer Ferreira; Hermann, Priscila Bacarin; Nascimento, Aguinaldo José do; Leonart, Maria Suely Soares

doi:10.5581/1516-8484.20130015

Cited by 46 publications

(31 citation statements)

References 19 publications

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“…Umarani et al [50] reported that the flavonoid rutin, also present in the EEHS, promoted the inhibition of lipid peroxidation in the heart tissue of rats subjected to oxidative stress. Henneberg et al [51] reported the ability of rutin to sequester reactive oxygen species in human erythrocytes subjected to oxidative damage. Protection against lipid peroxidation promoted by the EEHS may be associated with its direct role in the scavenging of peroxyl radicals or the modulation of endogenous antioxidant mechanisms, including the activation of the enzymes glutathione, catalase, and superoxide dismutase [2,48].…”

Section: Discussionmentioning

confidence: 99%

Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes

et al. 2016

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Hancornia speciosa Gomes (Apocynaceae) is a fruit tree, popularly known as mangabeira, and it is widely distributed throughout Brazil. Several parts of the plant are used in folk medicine, and the leaf and bark extracts have anti-inflammatory, antihypertensive, antidiabetic, and antimicrobial properties. In this study, we investigated the chemical composition of the ethanolic extract of Hancornia speciosa leaves (EEHS) and its antioxidant, antimicrobial, and cytotoxic activities as well as the mechanisms involved in cell death. The chemical compounds were identified by liquid chromatography coupled to mass spectrometry (LC-MS/MS). The antioxidant activity of the EEHS was investigated using the method that involves the scavenging of 2,2-diphenyl-1-picrylhydrazyl free radicals as well as the inhibition of oxidative hemolysis and lipid peroxidation induced by 2,2’-azobis (2-amidinopropane) in human erythrocytes. The antimicrobial activity was determined by calculating the minimum inhibitory concentration, minimum bactericidal concentration, minimum fungicidal concentration, and zone of inhibition. Kasumi-1 leukemic cells were used to assess the cytotoxic activity and mechanisms involved in cell death promoted by the EEHS. The chemical compounds identified were quinic acid, chlorogenic acid, catechin, rutin, isoquercitrin, kaempferol-rutinoside, and catechin-pentoside. The EEHS demonstrated antioxidant activity via the sequestration of free radicals, inhibition of hemolysis, and inhibition of lipid peroxidation in human erythrocytes incubated with an oxidizing agent. The antimicrobial activity was observed against American Type Culture Collection (ATCC) and hospital strains of bacteria and fungi, filamentous fungi and dermatophytes. The cytotoxic activity of the EEHS was induced by apoptosis, reduction of the mitochondrial membrane potential, and activation of cathepsins. Together, these results indicate the presence of phenolic compounds and flavonoids in the EEHS and that their antioxidant, antimicrobial, and cytotoxic activities in acute myeloid leukemia cells are mediated by apoptosis.

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Section: Discussionmentioning

confidence: 99%

Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes

et al. 2016

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“…In addition, these results showed that the anti-hemolysis effect could be linked not only to the polyphenols and flavonoids contents of the extract but also to the chemical structure of these compounds. It was demonstrated that binding of flavonoids to the red blood cell membranes significantly inhibits lipid peroxidation, and at the same time enhances their integrity against lysis [31].…”

Section: Blood Total Antioxidant Capacitymentioning

confidence: 99%

Antioxidant and Anti-Inflammatory Activities of Rubus Fruticosus and Zizyphus Vulgaris Methanol Extracts

Meziti

Bouriche

Hichem

et al. 2017

Int J Pharm Pharm Sci

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Objective: This report is an attempt to study the phenolic composition of Rubus fruticosus (RFE) and Zizyphus vulgaris (ZVE) methanol extracts and evaluate their antioxidant and anti-inflammatory effects in-vitro and in-vivo. Methods:Total phenolic and total flavonoids contents of extracts were determined by spectrophotometric methods. Phenolic compounds were identified by HPLC-TOF/MS. The antioxidant activities were evaluated in vitro using DPPH, ABTS and FRAP assays. The effect of RFE and ZVE on DNA cleavage induced by H2O2 Results:The phytochemical analysis of these extracts showed that RFE possesses higher polyphenolic and flavonoid content than ZVE. in the same way RFE exerted the highest antioxidant capacity with IC UV-photolysis was also investigated. The antioxidant effect of RFE and ZVE was tested in vivo using the blood total antioxidant capacity test in mice. On the other hand, the anti-inflammatory activity was assessed in vivo using two models of acute inflammation ear edema and vascular permeability. 50 value of 14 µg/ml in DPPH assay, 1.58 mmol of Trolox E/mg extract and 3.39 of mmol FesO4/mg extract in ABTS, and FRAP assay respectively. The studied extracts showed a concentration-dependent protective effect on DNA cleavage induced by H2O2 UV-photolysis. The daily oral administration of 200 mg/kg of RFE or ZVE during three weeks showed an improvement of the blood total antioxidant capacity; the HT50 Conclusion:The results obtained in this investigation showed that RFE possesses strong antioxidant and anti-inflammatory potential in comparison with ZVE, which may be attributed to the presence of polyphenolic phytoconstituents. values were151.45 min and 146.72 min for the groups treated with RFE and ZVE, respectively versus 122.5 min for the control group. The topical application of 2 mg/ear of RFE inhibited the croton oil-induced ear edema by 75.72%, while the inhibition exerted by ZVE was 64.24%. These inhibitions were higher than that of indomethacin, used as a reference. Moreover, the oral administration of 400 mg/kg of RFE inhibited significantly (33.57%) acetic acid induced vascular permeability in mice. However, this effect was lower than this of indomethacin. The inhibition effect exerted by ZVE was not significant.

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“…Myricetin was found with hepatoprotective properties against pyrogallolinduced toxicity. It prevented ROS production induced by tert-butylhydroperoxide in erythrocytes sampled from both normal subjects and sickle cell anemia patients treated with hydroxyurea (Henneberg et al, 2013;Matic et al, 2013). Myricetin also suppressed lipid peroxidation of hepatic microsomes derived from rats with deficient in D-atocopherol, which is a major lipid soluble antioxidant (Duthie & Morrice, 2012).…”

Section: Introductionmentioning

confidence: 97%

Attenuating effects of dihydromyricetin on angiotensin II-induced rat cardiomyocyte hypertrophy related to antioxidative activity in a NO-dependent manner

Meng

Yang

Yao

et al. 2014

Pharmaceutical Biology

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(2015) Attenuating effects of dihydromyricetin on angiotensin II-induced rat cardiomyocyte hypertrophy related to antioxidative activity in a NO-dependent manner, Pharmaceutical Biology, 53:6, 904-912, DOI: 10.3109/13880209.2014 Neonatal rat cardiomyocytes were pretreated with DMY (0-320 lM) followed with Ang II (100 nM) stimulation for 24 h, and then degree of hypertrophy was evaluated by cell surface analysis. Levels of reactive oxygen species (ROS) were measured with 2 0 ,7 0 -dichlorfluorescein-diacetate (DCFH-DA) fluorescent staining. Antioxidative activity was evaluated by malondialdehyde (MDA) level, superoxide dismutase (SOD) activity, and total antioxidant capacity (T-AOC). Cyclic guanosine monophosphate (cGMP) was determined with a radioimmunoassay. Results: Pre-incubation with DMY (20, 40, 80, and 160 lM) for 8 h, 12 h, 24 h, or 48 h decreased cell surface areas. It down-regulated mRNA expression of atrial natriuretic factor (1.95-to 1.24-fold) and b-myosin heavy chains (3.51-to 2.32-fold), reduced levels of MDA as well as increased SOD activity and T-AOC. Expression of SOD and thioredoxin were enhanced by DMY, whereas p22 phox and phosphorylation of mitogen-activated protein kinases were inhibited. Content of cGMP (0.54-to 0.80-fold) and phosphorylation of endothelial nitric oxide synthase at serine 1177 (0.70-to 1.05-fold) were augmented by DMY. Moreover, attenuating effect of DMY on hypertrophy was abolished when NO production was inhibited by L-NAME. Conclusion: Attenuating effects of DMY on Ang II-induced cardiomyocyte hypertrophy related to antioxidative activity in a NO-dependent manner. KeywordsCyclic guanosine monophosphate, endothelial nitric oxide synthase, mitogen-activated protein kinase, reactive oxygen species History

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Protective effect of flavonoids against reactive oxygen species production in sickle cell anemia patients treated with hydroxyurea

Cited by 46 publications

References 19 publications

Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes

Antioxidant, Antimicrobial and Cytotoxic Properties as Well as the Phenolic Content of the Extract from Hancornia speciosa Gomes

Antioxidant and Anti-Inflammatory Activities of Rubus Fruticosus and Zizyphus Vulgaris Methanol Extracts

Attenuating effects of dihydromyricetin on angiotensin II-induced rat cardiomyocyte hypertrophy related to antioxidative activity in a NO-dependent manner

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