Pseudomonas laurentiana sp. nov., an Mn(III)-oxidizing Bacterium Isolated from the St. Lawrence Estuary

Wright, Mitchell Henry; Hanna, J; Pica, Derek Anthony; Tebo, Bradley M.

doi:10.5530/pc.2018.4.32

Cited by 15 publications

(12 citation statements)

References 22 publications

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“…Incubation was achieved at 37°C for 72 h. All stock cultures were sub‐cultured and maintained in liquid media at 4°C. Culture purity was intermittently monitored using 16S rRNA sequencing using previously described methods .…”

Section: Methodsmentioning

confidence: 99%

Bioactive compounds sourced from Terminalia spp. in bacterial malodour prevention: an effective alternative to chemical additives

Cock

Wright²,

Matthews

et al. 2019

Intern J of Cosmetic Sci

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Objective Recently, our group reported that extracts prepared from the Australian native plant Terminalia ferdinandiana Exell. are potent inhibitors of the growth malodorous bacteria with similar efficacy to triclosan and through these results, we highlighted a potential biological alternative to the current chemical additives. Other members of the genus Terminalia are also well documented for their antibacterial potential and tannin contents and thus were investigated as potential deodorant additives. Methods Solvent extractions prepared from of selected Indian, Australian and South African Terminalia spp. were screened by disc diffusion and liquid dilution assays against C. jeikeium, S. epidermidis, P. acnes and B. linens. The antibacterial activity was quantified by liquid dilution MIC assays. The extracts were screened for toxicity using Atremia franciscana nauplii and HDF cell viability bioassays. High‐resolution time‐of‐flight (TOF) LC‐MS and GC‐MS headspace fingerprint analysis was used to detect tannin, flavonoid and terpenoid components in the extracts. Results Bacterial growth inhibition was observed in all Terminalia extracts with the methanolic T. chebula, T. carpenteriae and T. sericea extracts the most promising bacterial growth inhibitors, yielding MIC values as low as 200 µg mL−1. Toxicity analyses of the extracts were favourable, and we determined that the methanolic T. chebula, T. carpenteriae and T. sericea extracts were all non‐toxic. Using previously detected T. ferdinandiana antimicrobials as benchmarks, LC‐MS and GC‐MS fingerprint analyses revealed similar compounds in the methanolic T. chebula, T. carpenteriae and T. sericea extracts. Conclusion Through these results, we propose that Terminalia spp. extracts may be useful deodorant additives to inhibit the growth of axillary and plantar malodorous bacteria, offering a biological alternative to their chemically synthesized counterparts.

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Section: Methodsmentioning

confidence: 99%

Bioactive compounds sourced from Terminalia spp. in bacterial malodour prevention: an effective alternative to chemical additives

Cock

Wright²,

Matthews

et al. 2019

Intern J of Cosmetic Sci

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“…Of these, the Pseudomonas putida group, belonging to the P. fluorescens lineage, has been found to contain 24 species so far (Table S1, available in the online version of this article) [3–15]. These include Pseudomonas fulva , Pseudomonas parafulva and Pseudomonas cremoricolorata isolated from rice paddies, and Pseudomonas guariconensis and ‘ Pseudomonas qingdaonensis ’ derived from the rhizospheric soil of legumes.…”

Section: Full-textmentioning

confidence: 99%

“…Based on the results, they are now categorized into three lineages (Pseudomonas fluorescens, Pseudomonas aeruginosa and Pseudomonas pertucinogena lineages) and these lineages are further subdivided into groups and subgroups [3][4][5][6]. Of these, the Pseudomonas putida group, belonging to the P. fluorescens lineage, has been found to contain 24 species so far (Table S1, available in the online version of this article) [3][4][5][6][7][8][9][10][11][12][13][14][15]. These include Pseudomonas fulva, Pseudomonas parafulva and Pseudomonas cremoricolorata isolated from rice paddies, and Pseudomonas guariconensis and 'Pseudomonas qingdaonensis' derived from the rhizospheric soil of legumes.…”

mentioning

confidence: 99%

Pseudomonas brassicae sp. nov., a pathogen causing head rot of broccoli in Japan

Sawada

Fujikawa

Horita

2020

International Journal of Systematic and Evolutionary Microbiology

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Phytopathogenic bacteria, MAFF 212426, MAFF 212427T, MAFF 212428 and MAFF 212429, were isolated from head rot lesions of broccoli (Brassica oleracea L. var. italica Plenck) in Hokkaido, Japan, and subjected to polyphasic taxonomic characterization. The cells were Gram-reaction-negative, aerobic, non-spore-forming, motile with one or two polar flagella, rod-shaped and formed pale yellow colonies. Results of 16S rRNA gene sequence analysis showed that they belong to the genus Pseudomonas with the highest similarity to ‘Pseudomonas qingdaonensis’ JJ3T (99.86 %), Pseudomonas laurentiana GSL-010T (99.22 %), Pseudomonas huaxiensis WCHPs060044T (99.01 %), Pseudomonas japonica NBRC 103040T (98.87 %) and Pseudomonas alkylphenolica KL28T (98.73 %). The genomic DNA G+C content was 63.4 mol% and the major fatty acids (>5 % of the total fatty acids) were summed feature 3 (C16 : 1 ω7c / C16 : 1 ω6c), C16 : 0, summed feature 8 (C18 : 1 ω7c / C18 : 1 ω6c) and C17 : 0 cyclo. Multilocus sequence analysis using the partial rpoD, gyrB and rpoB gene sequences and phylogenomic analyses based on the whole genome sequences demonstrated that the strains are members of the Pseudomonas putida group, but form a monophyletic, robust clade separated from their closest relatives. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values corroborated their novel species status, with 88.39 % (ANI) and 35.8 % (dDDH) as the highest scores with ‘P. qingdaonensis’ JJ3T. The strains were differentiated from their closest relatives by phenotypic characteristics, pathogenicity on broccoli, and whole-cell MALDI-TOF mass spectrometry profiles. The phenotypic, chemotaxonomic and genotypic data showed that the strains represent a novel Pseudomonas species, for which the name Pseudomonas brassicae sp. nov. is proposed. The type strain is MAFF 212427T (=ICMP 23635T).

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“…veronii lineage. The type strain GSL-010 T of P. laurentiana was first isolated by Wright et al (2019) as a Mn(III)-oxidizing bacteria from the St. Lawrence Estuary. As stated above, no information can be found regarding petroleum hydrocarbon degradation ability of P. laurentiana pure cultures.…”

Section: Discussionmentioning

confidence: 99%

Aerobic and oxygen-limited naphthalene-amended enrichments induced the dominance of Pseudomonas spp. from a groundwater bacterial biofilm

Benedek

Szentgyörgyi

Szabó

et al. 2020

Appl Microbiol Biotechnol

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In this study, we aimed at determining the impact of naphthalene and different oxygen levels on a biofilm bacterial community originated from a petroleum hydrocarbon–contaminated groundwater. By using cultivation-dependent and cultivation-independent approaches, the enrichment, identification, and isolation of aerobic and oxygen-limited naphthalene degraders was possible. Results indicated that, regardless of the oxygenation conditions, Pseudomonas spp. became the most dominant in the naphthalene-amended selective enrichment cultures. Under low-oxygen conditions, P. veronii/P. extremaustralis lineage affiliating bacteria, and under full aerobic conditions P. laurentiana–related isolates were most probably capable of naphthalene biodegradation. A molecular biological tool has been developed for the detection of naphthalene 1,2-dioxygenase-related 2Fe-2S reductase genes of Gram-negative bacteria. The newly developed COnsensus DEgenerate Hybrid Oligonucleotide Primers (CODEHOP-PCR) technique may be used in the monitoring of the natural attenuation capacity of PAH-contaminated sites. A bacterial strain collection with prolific biofilm-producing and effective naphthalene-degrading organisms was established. The obtained strain collection may be applicable in the future for the development of biofilm-based bioremediation systems for the elimination of PAHs from groundwater (e.g., biofilm-based biobarriers).

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Pseudomonas laurentiana sp. nov., an Mn(III)-oxidizing Bacterium Isolated from the St. Lawrence Estuary

Cited by 15 publications

References 22 publications

Bioactive compounds sourced from Terminalia spp. in bacterial malodour prevention: an effective alternative to chemical additives

Bioactive compounds sourced from Terminalia spp. in bacterial malodour prevention: an effective alternative to chemical additives

Pseudomonas brassicae sp. nov., a pathogen causing head rot of broccoli in Japan

Aerobic and oxygen-limited naphthalene-amended enrichments induced the dominance of Pseudomonas spp. from a groundwater bacterial biofilm

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