Spectrophotometric methods for analysis of different dosage forms containing pyridoxine hydrochloride

Habib, Neven M.; Abdelwhab, Nada S.; Abdelrahman, Maha M.; Ali, Nouruddin W.

doi:10.5155/eurjchem.7.1.30-36.1350

Cited by 10 publications

(4 citation statements)

References 12 publications

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“…A literature review revealed that several approaches to the determination of CYZ have been identified, either in the formulation of raw materials or pharmaceuticals, either alone or in conjunction with other medications. These recorded analytical approaches include non-aqueous titration [1], spectrophotometric [9][10][11], TLC-densitometric [12][13][14], HPLC [15][16][17][18][19][20], capillary zone electrophoretic [21], and potentiometric [22][23][24][25] methods. Neither in its pharmaceutical formulation nor in biological fluids, have no spectrofluorimetric methods for determining CYZ been published.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric Approach for Quantification of Cyclizine in the Presence of its Toxic Impurities in Human Plasma; in silico Study and ADMET Calculations

et al. 2022

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Cyclizine (CYZ); an antiemetic compound; is widely misused for its euphoric or hallucinatory effects, either by oral or intravenous routes. The concomitant abuse of CYZ among addicted adolescents contributes to neuromuscular disorders that are life-threatening. Consequently, with the company of 1-Methylpiperazine (MPZ) and diphenylmethanol (DPM, Benzhydrol) as pharmacopoeia-reported CYZ impurities, a novel spectrofluorimetric assay for the detection of CYZ, has been established either in human plasma samples or in its parenteral formulation. The native fluorescence of CYZ has been investigated under various conditions. Different parameters affecting relative fluorescence intensity of CYZ including diluting solvent, surfactant, plasma protein solvent, and pH were studied and optimized. The linearity obtained between the fluorescence intensity at emission wavelength 350 nm after excitation at 244 nm and the corresponding CYZ concentrations was in the range 10–1000 ng/mL for measurement of CYZ either in pure form or in human plasma samples, with a appropriate correlation coefficient (r = 0.9999) and 3.10 ng/mL as the limit of detection and 9.41 ng/mL as the limit of quantitation. The suggested procedure was created and validated in accordance with ICH guidelines for quantification of CYZ either in its pure form or its dosage form, and FDA guidelines for the assay of CYZ in human plasma. Finally, in silico study and ADMET predictions were conducted for the studied drug impurities to estimate their pharmacokinetic behaviors. The results showed that both CYZ impurities have higher cellular permeability and maximum tolerated doses, DPM has higher BBB and CNS permeability than MPZ, while MPZ exceeds DPM in total clearance and volume of distribution. Graphic Abstract

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Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric Approach for Quantification of Cyclizine in the Presence of its Toxic Impurities in Human Plasma; in silico Study and ADMET Calculations

et al. 2022

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“…To the best of our knowledge, PYR was determined with either MEC or CYC using different analytical methods, such as spectrophotometry (Arayne, Sultana, Siddiqui, Zuberi, & Mirza, 2007; El‐Gindy, 2003; El‐Gindy, Emara, & Mostafa, 2004; Habib, Abdelwhab, Abdelrahman, & Ali, 2016; Sharma, Sharma, Saxena, & Talwar, 1989; Shinde, Sayyed, Chaudhari, Chaware, & Biyani, 2016), HPLC (Al‐Jallad, Al‐Kurdi, Badwan, & Jaber, 1999; Arayne, Sultana, & Siddiqui, 2008; El‐Gindy, Emara, & Mostafa, 2004; Li & Lu, 2005; Mao & Carr, 2001; Nawaz, 2013; Sher et al, 2014), and chemometric/UV methods (El‐Gindy, 2003; El‐Gindy, Emara, & Mostafa, 2004). Moreover, MEC was determined using LC stability‐indicating assay method (LC‐SIAM) in tablets (Peraman, Manikala, Kondreddy, & Yiragamreddy, 2015).…”

Section: Introductionmentioning

confidence: 99%

Impurity‐profiling UPLC methods for quantitative analysis of some antiemetics formulated with pyridoxine

Saad

Draz

Naguib

et al. 2022

Biomedical Chromatography

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“…Pyridoxine's anti-oxidative and anti-inflammatory capabilities may have a therapeutic effect in reducing the intensity of COVID-19 and its effects [8]. MZH has been determined with PYH using a variety of analytical methods, including spectrophotometry [9][10][11][12][13][14][15], HPLC [14,[16][17][18][19][20], and UV and/or chemometric approaches [9], according to inquiry of the literature.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of two drugs used as supportive treatment for COVID-19: comparative statistical studies and analytical ecological appraisal

et al. 2022

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Pharmaceutical quality control products (QC) demand quick, sensitive, and cost-effective methods to ensure high production at a low cost. Green analytical methods are also becoming more common in pharmaceutical research to cut down on the amount of waste that goes into the environment. Meclizine hydrochloride (MZH) and pyridoxine hydrochloride (PYH) are reported to be excellent for calming down COVID-19. As a result, the amount of MZH and PYH manufactured by multinational pharmaceutical organizations has increased considerably during the last several months. The present work proposes three environmentally friendly, straightforward, and sensitive spectrophotometric procedures for quantification of MZH in the presence of PYH in a pure and marketable formulations. The approaches under examination include ratio subtraction (RSM), induced dual wavelength (IDW), and Fourier self-deconvolution (FSD). PYH, on the other hand, was directly quantified at 290 nm. For both drugs, the procedures follow Beer’s law in the range of (5–50 µg/mL). The RSM, IDW, and FSD methods, as well as the zero-order approach for PYH, have all been verified in accordance with ICH standards. The ecological value of established methodologies was determined using four distinct ways: the national environmental methods index (NEMI), the analytical Eco-scale, the Analytical Greenness Metric (AGREE), and the green analytical process index (GAPI). Comparing the findings to those of the previously described spectrophotometric technique, no major changes were identified.

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Spectrophotometric methods for analysis of different dosage forms containing pyridoxine hydrochloride

Cited by 10 publications

References 12 publications

Spectrofluorimetric Approach for Quantification of Cyclizine in the Presence of its Toxic Impurities in Human Plasma; in silico Study and ADMET Calculations

Spectrofluorimetric Approach for Quantification of Cyclizine in the Presence of its Toxic Impurities in Human Plasma; in silico Study and ADMET Calculations

Impurity‐profiling UPLC methods for quantitative analysis of some antiemetics formulated with pyridoxine

Simultaneous analysis of two drugs used as supportive treatment for COVID-19: comparative statistical studies and analytical ecological appraisal

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