Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus

Ahmed, Sayed Abdel Kader; Al-Refai, Mahmoud

doi:10.5155/eurjchem.5.3.503-506.1070

Cited by 13 publications

(6 citation statements)

References 26 publications

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“…Phytochemical investigation has been carried out on M. indicus, but the data are still limited. The plant contains glycosides, coumarins, flavonoids, and aromatic compounds . 3‐Methoxyflavone present in the plant was found to inhibit cell growth .…”

Section: Introductionmentioning

confidence: 99%

Study of Phytochemicals of Melilotus indicus and Alpha‐Amylase and Lipase Inhibitory Activities of Its Methanolic Extract and Fractions in Different Solvents

2019

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Melilotus indicus is used for food and medicinal purposes. The present study aimed to explore its phytochemicals and antidiabetic and anti-obesity potential. Methanolic extract (ME) of the dried, ground herb, obtained by the Soxhlet method, was fractionated into hexane, chloroform, ethyl acetate, 1butanolic and aqueous fractions. Chromatographic techniques were used for further fractionation. The compounds identified by GC-MS included 3-O-methyl-D-glucose, 3-hydroxyspirost-8en-11-one, 1-phenanthrenecarboxylic acid-tetradecahydro-7-(2methoxy-2-oxoethylidene)-1,4a,8-triethyl-9-oxo-methylester, 3-(1-methylhept-1-enyl)-5-methyl-2,5-dihydrofuran-2-one, squalene, phytol, 4-(4-hydroxy-2,6,6-trimethyl-7-oxabicyclo[4.1.0] hept-1-yl-3-buten-2-one, 2,4-bis(1,1-dimethylethyl)phenol, geranyl isovalerate and thymol. The ME and its fractions displayed porcine pancreatic alpha-amylase inhibitory activity higher than Acarbose. Interestingly, they promoted the lipase activity showing opposite trend to that Orlistat. The plant may be used to control diabetes and in the in the promotion of lipase action.

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Section: Introductionmentioning

confidence: 99%

Study of Phytochemicals of Melilotus indicus and Alpha‐Amylase and Lipase Inhibitory Activities of Its Methanolic Extract and Fractions in Different Solvents

2019

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“…shows cytotoxic activity against different cancerous cell lines due to its various properties. 11 The findings of our previous studies showed that ethanolic extract of M. indicus (L.) All. and various other traditional plants and their active constituents have potent anti-inflammatory and ulcer healing properties.…”

Section: Introductionmentioning

confidence: 97%

Therapeutic Potential of Standardized Extract of Melilotus indicus (L.) All. and Its Phytochemicals against Skin Cancer in Animal Model: In Vitro, In Vivo, and In Silico Studies

et al. 2022

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Melilotus indicus (L.) All. is known to have anti-inflammatory and anticancer properties. The present study explored the in vivo skin carcinogenesis attenuating potential of ethanolic extract of M. indicus (L.) All. (Miet) in a 7,12-dimethylbenz[ a ]anthracene (DMBA)-induced skin cancer model. The ethanolic extract of the plant was prepared by a maceration method. HPLC analysis indicated the presence of quercetin in abundance and also various other phytoconstituents. DPPH radical scavenging assay results showed moderate antioxidant potential (IC 50 = 93.55 ± 5.59 μg/mL). A topical acute skin irritation study showed the nonirritant nature of Miet. Data for the skin carcinogenic model showed marked improvement in skin architecture in Miet and its primary phytochemicals (quercetin and coumarin) treated groups. Miet 50% showed comparable effects with 5-fluorouracil. Significant ( p < 0.05) anticancerous effects were seen in coumarin–quercetin combination-treated animals than in single agent (coumarin and quercetin alone)-treated animals. Chorioallantoic membrane (CAM) assay results showed the antiangiogenic potential of Miet. Treatment with Miet significantly down-regulated the serum levels of CEA (carcinoembryonic antigen) and TNF-α (Tumor necrosis factor-α). Data for the docking study indicated the binding potential of quercetin and coumarin with TNF-α, EGFR, VEGF, and BCL2 proteins. Thus, it is concluded that Miet has skin cancer attenuating potential that is proposed to be due to the synergistic actions of its bioactive molecules. Further studies to explore the effects of Miet and its bioactive molecules as an adjuvant therapy with low dose anticancer drugs are warranted, which may lead to a new area of research.

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“…Medicinal plants play an important role in the cancer prevention and treatment. Melilotus indicus extract (MIE) has recently been shown to suppress growth of several tumor cells including liver, breast, colon and cervix (Abu-Dahab and Afifi 2007;Ahmed and Al-Refai 2014). However, the mechanisms by which MIE initiates its anticancer activity are completely unknown.…”

Section: Discussionmentioning

confidence: 99%

Melilotus indicus extract induces apoptosis in hepatocellular carcinoma cells via a mechanism involving mitochondria-mediated pathways

et al. 2018

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Melilotus indicus, is a traditional medicine used as analgesic and emollient. Although Melilotus indicus extract (MIE) has recently been shown to suppress growth of several tumor cell lines, information regarding its antitumor mechanism is completely unknown. Here, we report the mechanism underlying the effects of MIE on human hepatocellular carcinoma cells, specifically HepG2, and SNU-182 cells. Methanolic MIE impaired the proliferation, and induced cell death in both HepG2 and SNU-182 cells but not in normal hepatic L-02 cells. Mechanistically, flow cytometric analysis revealed that MIE induces apoptosis in HepG2, and SNU-182 cells. However, MIE-induced apoptosis were not affected by a pan caspase inhibitor z-VAD-fmk as well as MIE did not stimulate caspase activation. Furthermore we found that MIE-induced apoptosis could be attributed to a mechanism involving mitochondria-mediated pathways evidenced by decrease in the mitochondrial membrane potential (ΔΨm), increase in the Bax/Bcl-2 ratio, and translocation of apoptosis inducing factor (AIF) from the mitochondria to the nucleus. Suppression in AIF expression by siRNA reduced MIE-induced apoptosis which suggested the dependency of MIE on AIF to induce apoptosis in hepatocellular carcinoma cells. To the best of our knowledge this is the first report elucidating the anticancer mechanism of MIE. Our findings suggested that MIE might be a good extract for developing anticancer drugs for human hepatocellular carcinoma treatment.

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Chemical constituents and cytotoxic activities of the extracts of Melilotus indicus

Cited by 13 publications

References 26 publications

Study of Phytochemicals of Melilotus indicus and Alpha‐Amylase and Lipase Inhibitory Activities of Its Methanolic Extract and Fractions in Different Solvents

Study of Phytochemicals of Melilotus indicus and Alpha‐Amylase and Lipase Inhibitory Activities of Its Methanolic Extract and Fractions in Different Solvents

Therapeutic Potential of Standardized Extract of Melilotus indicus (L.) All. and Its Phytochemicals against Skin Cancer in Animal Model: In Vitro, In Vivo, and In Silico Studies

Melilotus indicus extract induces apoptosis in hepatocellular carcinoma cells via a mechanism involving mitochondria-mediated pathways

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