A series of novel butenolide compounds containing oxime ether moiety were designed and synthesized through diversity modification of the benzene ring at 4-phenyl and the 3-oxime ether group based on the fungicidal 3-acetyl-4-phenyl-1-oxaspiro[4,5]dec-3-en-2-one oxime ether as the lead structure. Their structures were characterized by 1 H NMR, 13 C NMR, HR-ESI-MS data and X-ray diffraction. The preliminary in vitro bioassay results indicated that some compounds exhibited good fungicidal activities against Thanatephorus cucumeris, Sclerotinia sclerotiorum and Botrytis cinerea. For example, (E)-3-(1-((2-chlorobenzyloxy)imino)ethyl)-4-(pyridin-2-yl)-1-oxaspiro[4.5]dec-3-en-2-one (A7) had EC50 values of 6.02, 8.25 and 15.70 mg/L against T. cucumeris, S. sclerotiorum and B. cinerea, (E)-3-(1-((2-chlorobenzyloxy)imino)ethyl)-4-(pyridin-4-yl)-1-oxaspiro[4.5]dec-3-en-2-one (A8) had EC50 values of 1.31 and 10.62 mg/L against T. cucumeris and S. sclerotiorum, respectively. A7 had an excellent inhibition against spore germination of B. cinerea, and the inhibition rate was 95% at the concentration of 100 mg/L. The in vivo bioassay results showed that compounds A7 had a notable protective activity against tomato gray mould and rice sheath blight disease. Keywords 3-acetyl-4-aryl-1-oxaspiro[4,5]dec-3-en-2-one; oxime ether; synthesis; fungicidal activity 杀菌剂在农业生产中具有举足轻重的作用, 随着杀 菌剂的广泛使用, 病原菌产生了严重的抗药性, 如近年 来应用较多的甲氧基丙烯酸酯类杀菌剂 [1] , 已发现多种 植物病原菌对其产生了严重的抗药性 [2] . 我国在十三五 期间提出的农药减量使用和零增长目标已取得显著的 成效, 在十四五期间仍是农业重点研发计划的重要目 标. 研究开发低抗药性、生态友好、作用机制新颖的绿 色农药成为现代农业发展的迫切需求 [3] , 而发现作用方 式独特、化学结构新颖的农用杀菌剂是其重要发展方向 之一 [4] . 丁烯内酯属于 α,β-不饱和内酯类化合物, 又称 为 2(5H)-呋喃酮类化合物, 是一个具有优良生物活性的 结构单元, 广泛存在天然产物中, 如二萜内酯类化合物