Spectrofluorometric determination of linagliptin in bulk and in pharmaceutical dosage form

Abstract

KEYWORDSSimple and precise spectrofluorometric method has been developed and validated for the determination of linagliptin (LNG) in the range of 10-110 μg/mL. The results obtained were of good precision and statistically compared to the reference method using one-way analysis of variance (ANOVA). The method developed was satisfactorily applied to the analysis of the pharmaceutical formulation and proved to be specific and accurate for the quality control of linagliptin in its pharmaceutical dosage form. The development of spectrofluorometric method for the determination of LNG was of interest as no such methods have been reported.

Liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of Vildagliptin in rat plasma

“…The methods reported in the literature were based on UVspectrophotometry and RP-HPLC for the estimation of VG individually or combined with other APIs in pharmaceuticals [6][7][8][9]. Bio-analytical method using simple RP-HPLC has also been reported in the literature [10].…”

Development and validation of a reversed phase liquid chromatographic method for the determination of three Gliptins and Metformin in the presence of Metformin impurity (1-cyanoguanidine)

“…Figure in parentheses are the theoretical t value at (p = 0.05). b Reference method: aliquots of standard solutions in distilled water containing 2-10 μg/mL STG were measured using methanol as a blank [5]. No significant difference between groups of sitagliptin by using one way ANOVA with F = 0.04 and p = 0.85. c Reference method: aliquots of standard solutions in distilled water containing 5-25 μg/mL VDG were measured using water as a blank [5].…”

“…A liquid chromatographic (LC) method was reported for the determination of STG in ternary mixture with MET and sitagliptin degradation product [4]. For VDG, literature survey reveals that only one spectroscopic method and one LC method were reported for its determination [5,6]. SXG is recently approved for the treatment of type-2 diabetes mellitus [7].…”

Eco-Friendly Simultaneous Chromatographic Determination of Amiloride Hydrochloride, Atenolol, and Hydrochlorothiazide in Urine

“…Literature survey revealed that AT or AM or HZ was individually determined or in combination with other drugs by high-performance liquid chromatography (HPLC) [12][13][14][15][16][17][18][19][20][21][22]. The ternary mixture of AT, HZ, and AM was only determined by reversed-phase liquid chromatographic methods in pharmaceutical formulations using cyanopropyl and C18 columns [23,24]. HPLC-tandem mass spectrometry (MS/MS) and ultra-performance liquid chromatography (UPLC)-MS/MS methods were used for the simultaneous determination of diuretics and β-blockers in human urine [25] after pretreatment by a solid phase extraction (SPE).…”