Spectrophotometric methods for the simultaneous determination of paracetamol and dantrolene sodium in pharmaceutical dosage form

Abstract

KEYWORDSAccurate, precise and simple spectrophotometric methods have been developed and validated for the determination of paracetamol (PAR) and dantrolene sodium (DAS). Spectrophotometric methods including zero order, first derivative ( 1 D) and derivative ratio methods ( 1 DD) have been developed. The zero order spectrophotometric method was used for the determination of DAS in the range of 1-20 μg/mL by measuring the absorbance at 379 nm where PAR exhibits zero reading. 1 D and 1 DD methods were used for the determination of PAR in the range of 1.5-20.0 μg/mL by measuring the peaks amplitudes at 265.5 nm and 265.0 nm, respectively. The proposed methods were used to determine PAR in binary mixture with DAS in the laboratory prepared mixtures and in pharmaceutical dosage form. The results obtained were statistically evaluated and found to be accurate and precise and can be satisfactorily applied for the quality control analysis of the cited drugs.

Simultaneous determination of dantrolene with ibuprofen and diclofenac in plasma by HPLC-DAD: Application to comparative pharmacokinetic study

“…Reported methods for DNT, DIC and IBU assay in different matrices include spectrophotometric [9][10][11], spectrofluorimetric [5,12], densitometric [13,14], voltammetric [1,15,16], capillary isotachophoresis [17] and chromatographic methods [18][19][20][21][22][23].…”

Stability-Indicating Chromatographic Methods for the Determination of a Skeletal Muscle Relaxant and an Analgesic in their Combined Dosage Form

“…Few analytical methods have been described for the simultaneous determination of DNT and PAR including spectrophotometric [34][35][36] and HPLC [36,37] methods. Concerning stability indicating methods for determination of DNT and PAR binary mixture, there was only a reported gradient RP-HPLC method [37].…”