Simultaneous determination of Simvastatin and Sitagliptin in tablets by new univariate spectrophotometric and multivariate factor based methods

Abstract

KEYWORDSFive simple, sensitive and precise spectrophotometric and chemometric methods were used for simultaneous determination of Simvastatin (SM) and Sitagliptin (SIT) in their pure powdered forms and in the tablets. The proposed methods are the extended ratio subtraction method (EXRSM), ratio difference method (RDSM), mean centering of ratio spectra method (MCR) and chemometric methods, namely principal component regression (PCR) and partial least squares (PLS). In EXRSM; SM was determined at 237.5 nm, while SIT was determined at 267 nm, in RDSM; the difference in amplitudes at 237.5 and 245.5 nm was used for SM and 263.5 and 248.0 nm for SIT, while in MCR; SM and SIT were determined at 239.0 and 273.0 nm, respectively. PCR and PLS are factor based multivariate methods which utilize the whole spectra of SM and SIT. The developed methods were successfully applied for the determination of the studied drugs in their bulk powder, laboratory prepared mixtures and in tablets. All validation parameters of the developed methods were determined. The obtained results were statistically compared with each other along with a reported method.

Determination of binary mixture of ibuprofen and famotidine by different spectrophotometric methods

“…The main task of this work is to establish precise, simple, sensitive and accurate spectrophotometric analytical methods for simultaneous determination of IBU and FAM in their bulk powders and pharmaceutical dosage form with satisfactory precision and accuracy [17]. As well, to construct a statistical comparison between the ability of the proposed methods to determine both drugs in their pure form, laboratory prepared mixture and in their pharmaceutical formulations.…”

Universal procedures for spectrophotometric determination of anticoccidial drugs; application to multi-ingredient veterinary formulation and computational investigations for multivariate analysis

“…2021 -European Journal of Chemistry -CC BY NC -DOI: 10.5155/eurjchem.12.4.368-376.2144 For both PLS-2 and PCR, the concentration residuals were plotted against the actual concentrations of the prepared mixtures (Figures 7 and 8), and the residuals for all samples were found to be randomly distributed around zero [25][26][27][28][29][30][31][32][33]. The average recoveries of each drug using PLS-2 and PCR methods are summarized in Table 2.…”

“…The validation set is composed of 5 laboratory-prepared mixtures containing different proportions of AMP, SQX, and DVD (Table 1). In addition, the concentrations of AMP, SQX, and DVD in the veterinary formulation extract were calculated using the optimized PLS-2 or PCR calibration model after being recorded in the same specified lambda range [25][26][27][28][29][30][31][32][33].…”

TLC-spectrodensitometric method for simultaneous determination of dapagliflozin and rosuvastatin in rabbit plasma: stability indicating assay and kinetic studies

“…For examples, atorvastatin mainly used with glimepiride and metformin while simvastatin used with sitagliptin. [3][4][5] Some hypoglycemic drugs (e.g. glibenclamide) when taken with statins have the potential to increase the maximum plasma concentration (C max ) and the area under the curve (AUC) by up to 20%.…”