Composition and content of total phenolics and flavonoids, and antioxidant activity of Trigonella isthmocarpa

Güngör, Şerife Selma Uras; Ozay, Sevda Guzel; İlçım, Ahmet; Kökdil, Gamze

doi:10.5155/eurjchem.4.1.7-9.719

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…Studies have shown that some flavones, polyphenols, and phenolic acids have high antioxidant and anti-inflammatory activity [ 42 , 43 ]. In this study, the content and composition of flavones, polyphenols, and phenolic acid were measured.…”

Section: Resultsmentioning

confidence: 99%

Expression of Bioactive Lunasin Peptide in Transgenic Rice Grains for the Application in Functional Food

et al. 2018

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Lunasin, a bioactive peptide initially isolated from soybean, has anticancer, anti-inflammatory, and antioxidant activity. Due its great application value, lunasin seems to be a candidate gene in improving the nutritional value of crops. In this study, lunasin was inserted into the rice genome to evaluate whether it was feasible to express lunasin using the rice expression system and improve the bioactivity of protein in rice for our needs. We generatedlunasin-overexpressing rice lines, and chose three independent transgenic rice lines for further study. The lunasin content in trans-lunasin rice detected by UPLC-MS/MS was 1.01 × 10−3 g·kg−1 dry rice flour with grease removal in the lunasin extracts. The antioxidant efficacy of LET (lunasin-enriched fraction from trans-lunasin rice) and PEW (peptide-enriched fraction from wild type rice) was compared. Due to the presence of lunasin, LET showed higher (p < 0.05) antioxidant activity than PEW. LET exhibited high DPPH radical scavenging activity (IC50 value, 8 g·L−1), strong ABTS+ radical scavenging activity (IC50 value, 1.18 g·L−1), and great oxygen radical scavenging activity (170 μmol·L−1 Trolox equivalents when the concentration reached 4 g·L−1). Moreover, LET presented significantly higher (p < 0.05) anti-inflammatory activity on macrophage cells, and the NO production and the release of pro-inflammatory cytokines (IL-6, MCP1, and TNF-α) were significantly inhibited by LET. However, because of the low purity, LET showed weaker antioxidant and anti-inflammatory activity when compared to the Lunasin standard. These results suggested that it is feasible to use the rice expression system to express the exogenous lunasin in rice, and lunasin-overexpressing rice seems to be a candidate resource for application in functional food. Rice rich in lunasin is beneficial for human health, and could be used as a functional food in the diets of cancer and obese patients in the future.

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Section: Resultsmentioning

confidence: 99%

Expression of Bioactive Lunasin Peptide in Transgenic Rice Grains for the Application in Functional Food

et al. 2018

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“…Choosing the right extraction solvent is therefore fundamental in order to extract the compounds of interest selectively and in high amounts. This in turn has a direct impact on the outcome of the biological activity assays [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Influence of the physicochemical parameters of solvents in the extraction of bioactive compounds from Parinari macrophylla Sabine (Chrysobalanaceae)

et al. 2018

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The extraction of bioactive compounds from medicinal plants requires methods which are as diverse as the chemical nature of the compounds themselves. In this study, a 96-well microplate was used where solvent mixtures spanning wide ranges of selectivity and polarity were tested with the objective of extracting a broad range bioactive compounds from plant material. Microplate wells were filled with plant material and the solvents and their mixtures were added. The obtained extracts were assessed in terms of their total antioxidant activity, oxygen radical absorbance capacity and effects on cell viability. An aqueous extract, generally used by traditional therapists, was also included in the study. The results showed that the extracts using methanol with acetic acid (0.1%, v:v), chloroform/ethanol, butanol/DMF, butanol/acetonitrile, ethylene glycol with acetic acid (0.1%, v:v), MTBE/DMSO, ethylene glycol, pentane/ethanol (v:v), ethanol, DMF, DMF with acetic acid (0.1%, v:v), DMSO, DMSO with acetic acid (0.1%, v:v) and THF had a higher antioxidant activity than the aqueous extract. Extracts with greater antioxidant activity than the aqueous extract were obtained largely from solvent mixtures with the exception of ethanol, DMF, DMSO and THF. The antioxidant activity obtained in TEAC varied between 1474.1±4.4 and 3183.0±16.0 μmol TE/g dry extract respectively for aqueous and THF extracts; in ORAC between 1727.7±8.4 and 2683.5±11.7 μmol TE/g dry extract for aqueous and DMSO acetic acid 1%, respectively, with mean ±SEM. In TEAC the THF extract had the highest antioxidant potential with 3183.0±16.0 μmol TE / g dry extract. The DMSO acetic acid (0.1%, v:v) extract had the highest antioxidant potential in ORAC with 2683.5±11.7 μmol TE / g dry extract. Cell viability test using β-pancreatic cells showed that only the acidified methanol extract was toxic after one hour of incubation. After 24 hours, cell viability was less than 70% for extracts using butanol/acetonitrile, MTBE/DMF, acidified methanol, pentane/ethanol and acidified DMF.

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“…0.1 mL of the each extract and standard solutions of butylated hydroxyanisole (BHA) (200-500 mg/L) were added 2.9 mL the methanolic solution of DPPH. The mixtures were shaken vigorously and incubated in the dark for 45 min at room temperature and the decreases in the absorbance values were measured at 517 nm with a spectrophotometer [24]. All analysis was carried out in triplicate.…”

Section: Dpph Radical-scavenging Activitymentioning

confidence: 99%

Fatty acid, tocopherol, mineral composition, total phenolic, flavonoid and thymoquinone content, and antioxidant potential of Nigella stellaris

et al. 2014

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KEYWORDSIn the present study, the fatty acid composition of the fixed oil from seeds of Nigella stellaris has been investigated by GC-MS. Linoleic and oleic acids were the major fatty acids. The tocopherols determined by HPLC and α-and δ-tocopherols were detected in the oil. The seeds also were analyzed by ICP-MS to determine mineral content. Ca, P, and K were main elements among seventeen minerals in the seeds. Total phenolic and flavonoid contents of the aqueous methanolic extracts of different plant parts were assessed by Folin-Ciocalteu method and the AlCl3 assay. The seed extract was also analyzed by RP-HPLC analysis for its thymoquinone content. The present study showed that the seed extract contained low amount of thymoquinone. The seed extract exhibited the significantly higher total phenolic and flavonoid content than the extract of aerial part. DPPH radical scavenging activity was used to evaluate the antioxidant capacity of the extracts. Both of the extracts showed high antioxidant activity.

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Composition and content of total phenolics and flavonoids, and antioxidant activity of Trigonella isthmocarpa

Cited by 5 publications

References 15 publications

Expression of Bioactive Lunasin Peptide in Transgenic Rice Grains for the Application in Functional Food

Expression of Bioactive Lunasin Peptide in Transgenic Rice Grains for the Application in Functional Food

Influence of the physicochemical parameters of solvents in the extraction of bioactive compounds from Parinari macrophylla Sabine (Chrysobalanaceae)

Fatty acid, tocopherol, mineral composition, total phenolic, flavonoid and thymoquinone content, and antioxidant potential of Nigella stellaris

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