Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples

Faria, João Bosco; Dagnone, Ana Silvia; Munhoz, Thiago Demarchi; João, Carolina Franchi; Pereira, W. A. B.; Machado, Rosângela Zacarias; Tinucci‐Costa, Mirela

doi:10.4322/rbpv.01902006

Cited by 21 publications

(36 citation statements)

References 21 publications

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“…In the past various studies have been carried out regarding the molecular prevalence of E. canis worldwide and the prevalence rate has been reported to range from 3.1% to 88.0% (Murphy et al 1998;Dagnone et al 2003;Bulla et al 2004;Macieira et al 2005;Diniz et al 2007;Carvalho et al 2008;Alexandre et al 2009;Dagnone et al 2009;Faria et al 2010;Silva et al 2012). As regards the Indian scenario, a small number of published reports are available: the prevalence of E. canis was reported to be 50% (49/98) in Chennai (Lakshmanan et al 2007) and 20.6% from four different regions of India (Abd Rani et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Molecular prevalence and risk factors for the occurrence of canine monocytic ehrlichiosis

Milanjeet¹,

Singh²,

Singh³

et al. 2014

Vet. Med. - Czech

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Evaluation of blood samples collected from 214 dogs from Ludhiana, Punjab (India) was performed for the presence of Ehrlichia canis using PCR-based assays targeting a portion of the 16S rRNA gene. Of the total samples subjected to routine blood smear examination, the morulae of E. canis were detected in 2.34% samples. Nested PCR assay produced amplicons of expected size (389 bp) specific for E. canis in 41.59% (89/214) of samples. The results of multivariate analysis showed that the prevalence of E. canis was higher in the summer as compared to the winter season (P = 0.031) and in dogs younger than six-month-old as compared to older dogs (P < 0.001), while breed and sex of the host were not significantly associated with the occurrence of the disease.

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Section: Discussionmentioning

confidence: 99%

Molecular prevalence and risk factors for the occurrence of canine monocytic ehrlichiosis

Milanjeet¹,

Singh²,

Singh³

et al. 2014

Vet. Med. - Czech

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“…morulae in monocytes, macrophages and lymphocytes ( Figure 6) in Romanowsky-type stained smears from buffy coat and less frequently lymph node, BM, spleen, liver and cerebrospinal fl uid smears, is helpful in establishing a defi nitive diagnosis of acute CΜE [2,54,[58][59][60][61][62]. In a study with dogs naturallyinfected by E. canis (presumptive acute CME), the diagnostic sensitivity of buffy coat (review of 1000 oil immersion fi elds, 100x objective lens), lymph node (500 oil immersion fi elds, 100x objective lens) or their combination was 66%, 61% and 74%, respectively [59].…”

Section: Cytologymentioning

confidence: 99%

“…In a study with dogs naturallyinfected by E. canis (presumptive acute CME), the diagnostic sensitivity of buffy coat (review of 1000 oil immersion fi elds, 100x objective lens), lymph node (500 oil immersion fi elds, 100x objective lens) or their combination was 66%, 61% and 74%, respectively [59]. In another study, diagnostic sensitivity of spleen cytology in dogs naturally infected by E. canis was 49% [60]. Cytology may also support the diagnosis of CME even before seroconversion in acutely infected dogs and is valuable in documenting coinfections (e.g.…”

Section: Cytologymentioning

confidence: 99%

Canine Monocytic Ehrlichiosis: An Update on Diagnosis and Treatment

Mylonakis

Θεοδώρου

2017

Acta Veterinaria

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Canine monocytic ehrlichiosis (CME) is a tick-borne disease of worldwide distribution. The major causative agent is Ehrlichia canis, a gram-negative, obligate intracellular, pleomorphic bacterium of the genus Ehrlichia, which infects monocytes, macrophages and lymphocytes, forming intracytoplasmic, membrane-bound bacterial aggregates, called morulae. After an incubation period of 8-20 days, the course of E. canis infection, can be sequentially divided into acute, subclinical and chronic phases, although these phases can hardly be distinguished in the clinical setting. Clinical recovery is the typical outcome of acutely infected dogs, entering the subclinical phase, during which they show no or minimal clinical signs and/or mild hematological abnormalities. Immunocompetent dogs may eliminate the infection during the acute or subclinical phases, but an unpredictable proportion of dogs will eventually develop the chronic phase, characterized by aplastic pancytopenia and high mortality, due to septicemia and/or severe bleeding. This article outlines briefl y the pathogenesis of CME due to E. canis, and more thoroughly reviews the recent scientifi c literature pertaining to the diagnosis and treatment of this devastating disease.

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“…Canine ehrlichiosis is a disease with high incidence among domestic dogs in Brazil (DAGNONE et al, 2003;NAKAGHI et al, 2008;FARIA et al, 2010;VIEIRA et al, 2011), with extensive mortality. It is a tick-borne disease caused by Gram-negative bacteria, of the species Ehrlichia canis, mostly transmitted by Rhipicephalus sanguineus (LEWIS et al, 1977;TROY;FORRESTER, 1990).…”

Section: Introductionmentioning

confidence: 99%

Experimental Ehrlichia canis infection changes acute-phase proteins

Munhoz

Faria

Vargas-Hérnandez

et al. 2012

Rev. Bras. Parasitol. Vet.

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Early diagnosis of canine ehrlichiosis favors prompt institution of treatment and improves the prognosis for the animal, since this disease causes mortality among dogs. Studies have shown that determining the concentration of acute-phase proteins (APPs) may contribute towards early detection of disease and aid in predicting the prognosis. This study aimed to evaluate the APP profile in dogs experimentally infected with Ehrlichia canis, at the start of the infection and after treatment. It also investigated whether any correlation between APP levels and the clinical and laboratory alterations over the course of the disease would be possible. The results obtained showed abnormal levels of all the APPs on the third day after infection (D3), with the highest levels being reached on D18, with the exception of ceruloplasmin and acid glycoprotein, which presented their peaks on D6 and D12 respectively. We concluded that assessment of APP levels could contribute towards establishing an early diagnosis of canine ehrlichiosis, particularly regarding acid glycoprotein and ceruloplasmin, since these proteins were detected at increased levels even before the onset of clinical and laboratory findings of the disease.Keywords: Ehrlichia canis, experimental infection, acute-phase proteins, dogs. ResumoO diagnóstico precoce da erliquiose canina favorece a pronta instituição do tratamento e melhora o prognóstico do animal, pois se trata de uma doença de alta mortalidade em cães. Estudos têm apontado que a determinação da concentração de proteínas de fase aguda (PFA) pode contribuir para detecção precoce de doenças e auxiliar na predição do prognóstico. O presente estudo objetivou avaliar o perfil de proteínas de fase aguda (PFA) em cães experimentalmente infectados com Ehrlichia canis, no início da infecção e após o tratamento. Além disso, se seria possível associá-las com as alterações clínico-laboratoriais durante o curso da doença. Os resultados obtidos evidenciaram que todas as PFA estudadas alteraram suas concentrações em D3 (dia 3), comparadas ao D0, atingindo concentrações máximas em D18, com exceção da ceruloplasmina e da glicoproteína ácida, cujos picos foram observados em D6 e D12, respectivamente. Concluímos que a avaliação das concentrações de PFA poderiam contribuir para o diagnóstico precoce da erliquiose canina, principalmente com relação à ceruloplasmina e glicoproteína ácida, pois seus aumentos foram anteriores ao aparecimento dos sinais clínicos e das alterações laboratoriais da doença.Palavras-chave: Ehrlichia canis, infecção experimental, proteínas de fase aguda, cães.

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Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples

Abstract: Ehrlichia canis morulae and DNA detection in whole blood and spleen aspiration samples Detecção de mórulas e DNA de Ehrlichia canis em sangue e em aspirado de baço em cães naturalmente infectados

Cited by 21 publications

References 21 publications

Molecular prevalence and risk factors for the occurrence of canine monocytic ehrlichiosis

Molecular prevalence and risk factors for the occurrence of canine monocytic ehrlichiosis

Canine Monocytic Ehrlichiosis: An Update on Diagnosis and Treatment

Experimental Ehrlichia canis infection changes acute-phase proteins

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