2010
DOI: 10.4322/rbpv.01901005
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Abstract: Detection of anti-Toxoplasma gondii antibodies in experimentally and naturally infected non-human primates by Indirect Fluorescence Assay (IFA) and indirect ELISA Detecção de anticorpos anti-Toxoplasma gondii por meio das técnicas de Imunofluorescência Indireta e ELISA Indireto em primatas experimentalmente e naturalmente infectados

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Cited by 11 publications
(15 citation statements)
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“…En primates no humanos infectados experimentalmente, se detectó IgG e IgM a partir del día nueve de la infección; sin embargo, IgM fue detectable solo hasta el día 13 (Bouer et al, 2010). En general, se asume que la IgM anti-Toxoplasma solo se detecta en la fase inicial de la infección y es útil para el diagnóstico de exposición reciente o enfermedad aguda, mientras que IgG sugiere enfermedad crónica (Yano et al, 1983; Sedlak y Bartova, 2006).…”
Section: Discussionunclassified
“…En primates no humanos infectados experimentalmente, se detectó IgG e IgM a partir del día nueve de la infección; sin embargo, IgM fue detectable solo hasta el día 13 (Bouer et al, 2010). En general, se asume que la IgM anti-Toxoplasma solo se detecta en la fase inicial de la infección y es útil para el diagnóstico de exposición reciente o enfermedad aguda, mientras que IgG sugiere enfermedad crónica (Yano et al, 1983; Sedlak y Bartova, 2006).…”
Section: Discussionunclassified
“…Also, the frequency of IgM and IgG was different in these two methods. Detection of antibodies using the ELISA test is more sensitive than ones using the IFA test (17), therefore we decided to use the ELISA test in this study. In the study performed by Veeranootet al, ELISA test was used, and it was demonstrated that IgG was positive and IgM was negative in pregnant women (18); this is similar to the findings of our study.…”
Section: Discussionmentioning
confidence: 99%
“…Diluted serum specimens are incubated with killed Toxoplasma tachyzoites fixed on a glass slide to allow specific antigen-antibody interaction [8]. The interaction will then detected by addition of fluorescent-labeled anti-human IgG or IgM antibodies, and the result is viewed under a fluorescence microscope [18]. Although IFA is relatively inexpensive, fluorescence microscope is required, and the individual differences in result reading are considered as one of the test limitation.…”
Section: Indirect Fluorescent Assay (Ifa)mentioning
confidence: 99%