AvaliaÇão in vitro do fungo predador de nematoides Duddingtonia fagrans sobre larvas infectantes de ciatostomíneos de equinos (Nematoda: Cyathostominae)

Abstract

ResumoA capacidade predatória de um isolado de fungo predador de nematoides Duddingtonia flagrans (AC001) sobre larvas infectantes de ciatostomíneos foi avaliada em condições laboratoriais em ensaio experimental em meio ágar-água 2% (AA 2%). Houve redução significativa (p < 0,01) de 93,64% na média de larvas infectantes de ciatostomíneos recuperadas do meio AA2%, ao final de sete dias. Os resultados desse ensaio evidenciam que o isolado fúngico AC001 poderia ser utilizado no controle biológico de ciatostomíneos de equinos.Palavras-chave: Controle biológico, Strongyloidea, fungos nematófagos, fungos predadores de nematoides, controle alternativo. AbstractThe predatory capacity of one isolate of nematode-trapping fungus Duddingtonia flagrans (AC001) on infective larvae of cyathostomes was evaluated in laboratorial conditions in medium water-agar 2% (WA 2%). There was significant reduction (p < 0. 01) of 93.64% in the average of infective larvae of cyathostomes recovered of medium WA 2% at seven day. These results show that the isolated AC001 could be used in the biological control of cyathostomes of horses.

Predatory activity of nematophagous fungi on infective larvae of Ancylostoma sp.: evaluation in vitro and after passing through the gastrointestinal tract of dogs

“…The predatory fungal species used in this study have been reported as effective against parasitic nematodes in bovines (Arau ´jo et al, 1993(Arau ´jo et al, , 1998Alves et al, 2003), small ruminants (Mota et al, 2000;Chandrawathani et al, 2003;Arau ´jo et al, 2006b) and equines (Castro et al, 2003). Maciel et al (2006) evaluated in in vitro tests the predatory capacity of A. robusta (isolate I-31), D flagrans.…”

Optimizing protease production from an isolate of the nematophagous fungusDuddingtonia flagransusing response surface methodology and its larvicidal activity on horse cyathostomins

“…According to Braga et al (2009b), D. flagrans (AC001) has been used successfully under laboratory conditions in the biological control of gastrointestinal nematodes of humans and animals. In another study, Braga et al (2009c) reported that the isolate AC001 of D. flagrans grown in 2% water-agar medium showed predatory activity against cyathostomin L3, with a percentage reduction of 97.5% under laboratory conditions. The destruction of cyathostomins was confirmed with the use of optimized D. flagrans (AC001) enzymatic extract in this work.…”

Nematophagous fungi and their influences on parasitic control in sheep

“…With the growing demand on the part of producers for new alternatives in the control of verminosis in sheep, sustainable alternatives have aroused interest in the various product segments. To this detriment, biological control proves very interesting in combating worms in various forms, citing the use of predatory fungi of nematode larvae, which can attack parasitic populations directly in the soil, performing an alternative control (Araújo et al, 2004, Braga et al, 2009.…”

“…The species Duddingtonia flagrans is still considered the most promising due to the abundance of chlamydospores, ability to resist passage through the gastrointestinal tract, and efficient soil colonization, as well as being the most studied in the control of helminths of ruminants (Mota et al, 2003, Braga et al, 2009, Paraud et al, 2005. Faedo et al (1998) reinforce that Duddingtonia spp consists of the most resistant genus in existence when it comes to nematophagous species, which would make it a versatile alternative.…”