Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR

Cabral, Aline Diniz; Camargo, Clarice Neves; Galleti, Nara Thiers Cacciatori; Okuda, Líria Hiromi; Pituco, Edviges Maristela; Fava, Cláudia Del

doi:10.4322/rbpv.01804003

Cited by 23 publications

(25 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In goat fetuses, Eleni et al (2004) observed nonsuppurative meningoencephalitis and multifocal necrosis associated with N. caninum cysts in the cortex area. Histological consequences of N. caninum infection in heart and brain of cattle fetuses are similar to those described for the sheep fetuses investigated in present study (Corbelliniet al 2000, Pescador et al 2007, Cabral et al 2009). …”

Section: Resultssupporting

confidence: 71%

Sheep abortion associated with Neospora caninum in Mato Grosso do Sul, Brazil

et al. 2012

View full text Add to dashboard Cite

Pesq. Vet. Bras. 32(8) Canids are the main hosts of Neospora caninum, but cattle, (sheep, goats and horses may serve as intermediary hosts. N. caninum infection of pregnant intermediary hosts may provoke abortion and neonatal infections. This study is the ϐirst to report lamb abortion associated with N. caninum in Mato Grosso do Sul. Epidemiological data were obtained from interviews with sheep producers. For microscopic examination, fragments of different organs removed from 4 sheep fetuses, aborted and necropsied, were ϐixed in 10% formaldehyde, embedded in parafϐin and subjected to the hematoxylin-eosin staining protocol and immunohistochemistry (IHC) to test for N. caninum and Toxoplasma gondii. The abortion outbreak studied was reported from a herd of 268 Santa Inês sheep (including 186 pregnant ewes), with 10 abortion cases in the last third of gestation. Four fetuses were examined, 3 from a same ewe. At necropsy, one fetus exhibited crackling in the lung and all its organs were reddish. Histological ϐindings detected mononuclear cell inϐiltrates among myocardium ϐibers and around blood vessels, in addition to circular structures with basophilic points resembling protozoans. IHC tests revealed strongly positive staining for N. caninum and weakly positive for T. gondii, characterizing N. caninum infection.

show abstract

Section: Resultssupporting

confidence: 71%

Sheep abortion associated with Neospora caninum in Mato Grosso do Sul, Brazil

et al. 2012

View full text Add to dashboard Cite

show abstract

“…These findings are in agreement with a study in Argentina (moore et al, 2008) where none of the 70 fetuses were positive by nested-PCR, although low rates of T. gondii infection have been reported in other countries (ellis, 1998;GoTTsTein et al, 1998;saGer et al, 2001;CanaDa et al, 2002;reiTT et al, 2007). Cabral et al (2009), using the same 105 aborted fetuses of this study, confirmed the presence of N. caninum in 24.8% by IHC and nested PCR. The specificity of the nested PCR was showed by a BLAST analysis, that confirmed the primers of the ITS1 region were specific to this parasite, so no cross reaction occurred with other coccidians.…”

Section: Palavras-chavesupporting

confidence: 82%

Screening for Toxoplasma gondii in aborted bovine fetuses in Brazil

et al. 2013

Self Cite

View full text Add to dashboard Cite

The aim of this study was to determine if Toxoplasma gondii was present in aborted bovine fetuses in Brazil. Histopathology of 105 cases with suspected infectious abortion, analyzed during the period from 2006 to 2008 at Centro de Pesquisa e Desenvolvimento de Sanidade Animal of Instituto Biológico, São Paulo, showed 75 cases with indications of abortion due to apicomplexan protozoa. These cases were submitted to PCR for verification of the laboratory diagnosis. Fetal DNA was extracted from central nervous system, heart, liver, muscle, and/or placenta samples to obtain a 529 bp DNA fragment. T. gondii DNA was not detected in any of the bovine fetuses analyzed, suggesting that it may not be a frequent cause of bovine abortion.

show abstract

“…Overall, N. caninum was the predominant (21.3% of 258) cause of abortion in this investigation. A similar conclusion was reached by Cabral et al (2009), who combined histopathology, IHC, and PCR to diagnose N. caninum in 24.8% of 105 fetal samples from the state of SP. They detected N. caninum DNA in the brains of 22% (16 of 72), placenta of 20.0% (4 of 20), heart and liver of 16.3% (8 of 49) and pool of kidney, lungs, and spleen of 10.9% (7 of 64).…”

Section: Clinical Infectionsmentioning

confidence: 61%

All about neosporosis in Brazil

Cerqueira‐Cézar

Calero‐Bernal

Dubey

et al. 2017

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Neospora caninum is protozoan parasite with domestic and wild dogs, coyotes and grey wolves as the definitive hosts and many warm-blooded animals as intermediate hosts. It was cultivated and named in 1988. Neosporosis is a major disease of cattle and has no public health significance. Since 1990's N. caninum has emerged as a major cause of abortion in cattle worldwide, including in Brazil. N. caninum also causes clinical infections in several other animal species. Considerable progress has been made in understanding the biology of N. caninum and there are more than 200 papers on this subject from Brazil. However, most of the reports on neosporosis from Brazil are serological surveys. Overall, little is known of clinical neosporosis in Brazil, particularly cattle. The few reports pertain to sporadic cases of abortion with no information on epidemics or storms of abortion. The objective of the present review is to summarize all reports from Brazil and suggest topic for further research, including prevalence of N. caninum oocysts in soil or in canine feces, and determining if there are additional definitive hosts, other than the domestic dog. There is need for a national survey in cattle using defined parameters. Future researches should focus on molecular characterization of N. caninum strains, possibility of vaccine production and relationship between wildlife and livestock epidemiology.Keywords: Neospora caninum, neosporosis, domestic animals, wild animals, Brazil. ResumoNeospora caninum é um protozoário parasita que possui os canídeos domésticos e selvagens, coiotes e lobos cinzentos como hospedeiros definitivos e vários animais de sangue quente como hospedeiros intermediários. Foi cultivado e nomeado em 1988. A neosporose é uma das principais doenças em bovinos e não tem significância em saúde pública. Desde 1990, N. caninum tem emergido como uma das principais causas de aborto em bovinos em todo o mundo, inclusive no Brasil. N. caninum também causa infecções clínicas em várias outras espécies animais. Consideráveis avanços foram feitos na compreensão da biologia desse parasita e há mais de 200 trabalhos sobre o assunto no Brasil. No entanto, a maioria dos relatos de neosporose do Brasil são relacionados a sorologia. Em geral, pouco se sabe sobre a neosporose clínica no Brasil, particularmente em bovinos. Os poucos relatos referem-se a casos esporádicos de aborto sem informações sobre epidemias ou surtos de aborto. O objetivo da presente revisão é resumir todos os relatos sobre N. caninum no Brasil e sugerir tópicos para pesquisas futuras, incluindo a prevalência de oocistos de N. caninum no solo ou em fezes caninas e determinar se há hospedeiros definitivos adicionais, exceto o cão doméstico no país. Uma pesquisa nacional em bovinos usando parâmetros definidos seria de grande importância. Pesquisas futuras deveriam ser focadas na caracterização de cepas de N. caninum, possibilidade de produção de vacinas e a relação epidemiológica entre a vida selvagem e o gado.Palavras-chave: Neospora caninum, neospo...

show abstract

Diagnosis of Neospora caninum in bovine fetuses by histology, immunohistochemistry, and nested-PCR

Cited by 23 publications

References 36 publications

Sheep abortion associated with Neospora caninum in Mato Grosso do Sul, Brazil

Sheep abortion associated with Neospora caninum in Mato Grosso do Sul, Brazil

Screening for Toxoplasma gondii in aborted bovine fetuses in Brazil

All about neosporosis in Brazil

Contact Info

Product

Resources

About