Atividade ovicida e larvicida in vitro do óleo essencial de Eucalyptus globulus sobre Haemonchus contortus

Macedo, Iara Térsia Freitas; Beviláqua, Cláudia Maria Leal; Oliveira, Lorena Mayana Beserra de; Camurça-Vasconcelos, A.L.F.; Vieira, L. da S.; Oliveira, Fabrício R.; Queiroz-Junior, Eudson M.; Portela, Bruno G.; Barros, Renata Simões; Chagas, Ana Carolina Souza

doi:10.4322/rbpv.01803011

Cited by 43 publications

(19 citation statements)

References 20 publications

(22 reference statements)

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“…Aqueous extract of Annona senegalensis leaves inhibited 11.5% of egg hatching at a concentration of 7.1 mg.mL -1 (ALAWA et al, 2003). The maximum effectiveness of essential oil of Eucalyptus globulus on eggs was 99.3% at a concentration of 21.75 mg.mL -1 and on larvae was 98.7% at a concentration of 43.5 mg.mL -1 (MACEDO et al, 2009). Since the in vitro tests presented good results, the essential oil was subjected to toxicological assessment to evaluate its effect and to estimate the dose on live organisms.…”

Section: Discussionmentioning

confidence: 91%

Evaluation of Eucalyptus citriodora essential oil on goat gastrointestinal nematodes

Macedo

Beviláqua

Oliveira

et al. 2011

Rev. Bras. Parasitol. Vet.

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Phytotherapy may be an alternative strategy for controlling gastrointestinal parasites. This study evaluated the anthelmintic efficacy of Eucalyptus citriodora essential oil (EcEO). The in vitro effects of EcEO were determined through testing the inhibition of egg hatching and larval development of Haemonchus contortus. EcEO was subjected to acute toxicity testing on mice, orally and intraperitoneally. The in vivo effects of EcEO were determined by the fecal egg count reduction test (FECRT)

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Section: Discussionmentioning

confidence: 91%

Evaluation of Eucalyptus citriodora essential oil on goat gastrointestinal nematodes

Macedo

Beviláqua

Oliveira

et al. 2011

Rev. Bras. Parasitol. Vet.

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“…citratus essential oil was more efficient than the decoction and exhibited an efficacy superior to those of other essential oils tested previously. The EC50 values of Eucalyptus globulus essential oil were 8.3 and 6.9 mg/mL for egg hatching and larval development, respectively (MACEDO et al, 2009). The EC50 values of Lippia sidoides essential oil were 0.4 and 2.97 mg/mL for eggs and larvae, respectively (CAMURÇA-VASCONCELOS et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Anthelmintic activity of Cymbopogon citratus against Haemonchus contortus

Macedo

Oliveira

Ribeiro

et al. 2015

Rev. Bras. Parasitol. Vet.

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Parasitic nematodes are of major economic importance in livestock. An alternative for the control of parasites is phytotherapy. This study evaluated the efficacy of Cymbopogon citratus decoction (CcD), C. citratus essential oil (CcEo) and citral against Haemonchus contortus using in vitro egg hatch test (EHT) and larval development test (LDT) and an in vivo test using a Meriones unguiculatus (gerbil) model. The effect of 800 mg/kg CcEo was evaluated in gerbils that had been artificially infected with 5,000 third-stage H. contortus larvae. The effective concentrations required to inhibit 50% (EC50) of egg hatching were 0.46, 0.14 and 0.13 mg/mL for CcD, CcEo and citral, respectively. The EC50 values in the LDT were 5.04, 1.92 and 1.37 mg/mL for CcD, CcEo and citral, respectively. H. contortus population in the group treated with C. citratus essential oil was reduced by 38.5% (P< 0.05) in comparison to the control group. These results suggest that it may be possible to use C. citratus essential oil to control of H. contortus parasite of small ruminant.Keywords: Phytotherapy, anthelmintic, Meriones unguiculatus, in vitro, citral. ResumoO parasitismo por nematoides tem grande importância econômica no rebanho. Uma alternativa para o controle de parasitas é a fitoterapia. Este estudo avaliou a eficácia do decocto de Cymbopogon citratus (DCc), do óleo essencial de C. citratus (OECc) e do citral contra Haemonchus contortus utilizando o teste in vitro de eclosão dos ovos (TEO) e o teste de desenvolvimento larval (TDL) e um teste in vivo com modelo Meriones unguiculatus (gerbil). O efeito de 800 mg/kg de OECc foi avaliado em gerbils infectados artificialmente com 5000 larvas de terceira fase de H. contortus. As concentrações efetivas necessárias para inibir 50% (CE50) da eclosão dos ovos foram 0,46; 0,14 e 0,13 mg/mL para DCc, OECc e citral, respectivamente. Os valores da CE50 no TDL foram de 5,04; 1,92 e 1,37 mg/mL para DCc, OECc e citral, respectivamente. No grupo tratado com óleo de C. citratus a população H. contortus foi reduzida em 38,5% (P < 0,05) em comparação com o grupo controle. Estes resultados sugerem que pode ser possível a utilização de óleo essencial de C. citratus para controle de H. contortus, parasita de pequenos ruminantes.Palavras-chave: Fitoterapia, anti-helmínticos, Meriones unguiculatus, in vitro, citral.

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“…Extratos aquosos de Leonotis ocymifolia, Leucas martinicensis, Albizia schimperiana e Senna occidentalis induziu 100, 99,85, 99,31 e 96,36% de inibição do desenvolvimento larval de H. contortus, respectivamente (Egaule et al, 2011). Em estudo conduzido por Macedo et al (2009), utilizando óleo essencial de Eucalyptus globulus na concentração de 43,5mg/mL, foi demonstrado 98,7% de inibição do desenvolvimento larval. Extrato aquoso de Musa sp., avaliado na concentração de 57,76mg/mL apresentou eficácia de 90% (Oliveira et al, 2010).…”

Section: Resultsunclassified

Atividade ovicida e larvicida do extrato etanólico de Aloe vera L. sobre Haemonchus contortus

Sprenger¹

2015

RBCV

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ResumoO objetivo deste trabalho foi determinar a eficácia in vitro do extrato etanólico de Aloe vera L. frente a estágios de vida livre de Haemonchus contortus. O fitoterápico foi produzido com percolação da polpa do vegetal com álcool absoluto, a 25ºC, sendo posteriormente liofilizado. Foram realizados o teste de eclodibilidade de ovos (TEO) e o teste de desenvolvimento larval (TDL), com concentrações crescentes (0,78 a 100mg/mL) em seis repetições. Para analisar a composição química do fitoterápico, procedeuse a marcha fitoquímica completa, analisando qualitativamente a presença de fenóis, taninos, antocianinas, antocianidinas, leucoantocianidinas, estero ides, triterpenos, saponinas, resinas e alcaloides; além da quantificação de fenóis totais, teste de toxicidade frente Artemia salina e ensaio antioxidante pelo teste de redução do radical DPPH. O produto obtido a partir de A. vera apresentou eficácia de 94,35 ± 1,13% no TEO, já no TDL a eficácia foi de 76,03 ± 0,45%, ambos na concentração de 100mg/mL. Nas análises fitoquímicas, foram encontrados diversos compostos que podem ter contribuído com o efeito anti-helmíntico, tanto direta como indiretamente. Os dados da marcha fitoquímica, aliados aos resultados dose-dependentes obtidos nos testes in vitro evidenciam que o extrato produzido possui potencial para combater H. contortus. Novos estudos devem ser realizados buscando maximizar a eficácia deste extrato, uma vez que foram encontrados resultados excelentes nos testes realizados.Palavras-chave: Etnoveterinária, fitoterapia, infecção parasitária. AbstractThe aim of this study was to determine the in vitro efficacy of ethanolic extract of Aloe vera L. against Haemonchus contortus free-living stages. The herbal material was produced by pulp leaving the solution with ethanol at 25°C and latter the material was lyophilized. After this, the product was used for the egg hatch test (EHT) and the larval development test (LDT) in different concentrations (0.78 to 100mg/mL) with six replicates. A complete phytochemical screening was performed to analyze the materials' chemistry composition, screening qualitatively the presence of phenols, tannins, anthocyanins, anthocyanidins, leucoanthocyanidins, steroids, triterpenes, saponins, resins and alkaloids; beyond the total phenols quantification, brine shrimp toxicity test and DPPH radical reduction antioxidant test. A. vera extract had an efficacy of 94,35 ± 1,13% using the EHT and of 76,03 ± 0,45% when using the LDT test, both in concentration 100mg/mL. Phytochemical tests showed few chemical compounds that could exert anthelmintic properties. The results obtained with the biochemical tests together with the dose-dependent effect found in in vitro tests demonstrate that extract has the potential to be a good drug candidate against H. contortus. Further studies should be conducted to maximize the effectiveness of the herbal, because it demonstrated excellent results in experiments.Keywords: Ethnoveterinary, herbal medicine, parasitic infection. IntroduçãoA produção de pequ...

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Atividade ovicida e larvicida in vitro do óleo essencial de Eucalyptus globulus sobre Haemonchus contortus

Cited by 43 publications

References 20 publications

Evaluation of Eucalyptus citriodora essential oil on goat gastrointestinal nematodes

Evaluation of Eucalyptus citriodora essential oil on goat gastrointestinal nematodes

Anthelmintic activity of Cymbopogon citratus against Haemonchus contortus

Atividade ovicida e larvicida do extrato etanólico de Aloe vera L. sobre Haemonchus contortus

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