A review of the occurrence of hemoplasmas (hemotrophic mycoplasmas) in Brazil

Biondo, Alexander Welker; Santos, Andrea Pires dos; Guimarães, Ana Márcia Sá; Vieira, Rafael Felipe da Costa; Vidotto, Odilon; Macieira, Daniel de Barros; Almosny, Nádia Regina Pereira; Molento, Marcelo Beltrão; Timenetsky, Jorge; Morais, Helio Autrán de; González, Félix Hilario Díaz; Messick, Joanne B.

doi:10.4322/rbpv.01803001

Cited by 65 publications

(52 citation statements)

References 27 publications

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“…Os hemoplasmas têm sido frequentemente detectados em esfregaço sanguíneo de gatos domésticos no Brasil, embora alguns trabalhos que investigam a ocorrência desses agentes apontem a PCR como o método de eleição para o diagnóstico direto, em virtude de sua maior sensibilidade e especificidade (Moraes et al 2007, Biondo et al 2009, Sykes 2010. Através da PCR pode-se detectar infecções com baixa carga parasitária comuns em estudos epidemiológicos onde os animais são selecionados de forma randômica (Foley et al 1998).…”

Section: Discussionunclassified

Diagnóstico molecular da infecção por hemoplasmas em gatos domésticos naturalmente infectados da cidade de Belém, Pará

et al. 2013

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Pesq. Vet. Bras. 33(9):1116-1120 Mycoplasma haemofelis, 'Candidatus Mycoplasma haemominutum' and 'Candidatus Mycoplasma turicensis' are the causative agent of the feline mycoplasmosis, which could cause acute or chronic anemia. The aim of this work was to determine the occurrence of hemoplamas in domestic cats from Belém, Pará. To this, 201 cats were divided into three groups: Group A were composed by 101 stray cats captured by Zoonosis Control Center, group B were composed by 62 owners healthy cats and group C were composed by 38 owners cats that were suffering by some medical condition. Blood samples were collected to perform Polymerase Chain Reaction (PCR) to detect the DNA of these agents, which were sequenced and aligned. Statistical analysis was performed to detect association between the infection, the sex of the animals and experimental groups. The DNA of at least one of the hemoplasmas studied were detected in 19,9% (40/201) of the samples, being the DNA of 'Candidatus M. haemominutum' was found in 7.96% (16/201) of samples, M. haemofelis in 1.49% (3/201) of samples, while 'Candidatus M. turicensis' in 12.93% (26/201) of the samples. The DNA of these three agents was detected in cats from groups A and C, while in Group B was detected only 'Candidatus M. turicensis' and 'Candidatus M. haemominutum'. The influence of sex on hemoplasma infection was detected only between 'Candidatus M. haemominutum' and males. These findings showed that hemoplasma circulate among domestic cats in Belém, and 'Candidatus M. turicensis' and 'Candidatus M. haemominutum' were more common than M. haemofelis, especially in stray cats.

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Section: Discussionunclassified

Diagnóstico molecular da infecção por hemoplasmas em gatos domésticos naturalmente infectados da cidade de Belém, Pará

et al. 2013

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“…Subsequently, the same "Candidatus" species was found in other dogs in the United States (27) and in canids from Brazil (28), Europe (8,(29)(30)(31)(32), and Australia (33)(34)(35). Although ticks, in particular Rhipicephalus sanguineus (the brown dog tick), have been suggested as vectors for canine hemotropic mycoplasma transmission, there are no experimental data to support vector competence by any tick species (8,28,30,32,33,35,36). Interestingly, in this study, Mycoplasma ovis-like DNA was amplified only from blood samples, whereas "Candidatus Mycoplasma haematoparvum" was amplified from both blood and serum samples.…”

Section: Discussionmentioning

confidence: 99%

Infection with Hemotropic Mycoplasma Species in Patients with or without Extensive Arthropod or Animal Contact

et al. 2013

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b PCR amplification targeting the 16S rRNA gene was used to test individuals with and without extensive arthropod and animal contact for the possibility of hemotropic mycoplasma infection. The prevalence of hemotropic mycoplasma infection (4.7%) was significantly greater in previously reported cohorts of veterinarians, veterinary technicians, spouses of veterinary professionals, and others with extensive arthropod exposure and/or frequent animal contact than in a previously reported cohort of patients examined by a rheumatologist because of chronic joint pain or evidence of small-vessel disease (0.7%). Based upon DNA sequence analysis, a Mycoplasma ovis-like species was the most prevalent organism detected; however, infection with "Candidatus Mycoplasma haematoparvum" and a potentially novel, but incompletely characterized, hemotropic Mycoplasma species was also documented. Historical exposure to animals and arthropod vectors that can harbor hemotropic Mycoplasma spp. should be considered during epidemiological investigations and in the evaluation of individual patients.

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“…are uncultivated, small, pleomorphic, wall-less bacteria that parasitize on the surface of animal erythrocytes [1–3]. Hemoplasmas have now been reclassified into the Mycoplasma genus based on 16S rRNA gene sequence.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of hemotropic mycoplasmas (Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’) in sheep and goats in China

et al. 2017

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BackgroundHemotropic mycoplasmas (hemoplasmas) are emerging zoonotic pathogens with a worldwide distribution that can cause mild to severe hemolytic anemia, icterus, ill-thrift, infertility, and poor weight gain. However, understanding of the molecular epidemiology of hemoplasmas (Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’) is limited in sheep and goats, and the hemoplasma strain/species/variant ‘Candidatus M. haemovis’ was poorly studied throughout the world and had never been detected in China until now. Thus, the aim of the present study was to determine the molecular prevalence of hemoplasmas, including M. ovis and ‘Candidatus M. haemovis’ in sheep and goats from seven provinces and one autonomous region of China.MethodsA total of 1364 blood samples were collected from sheep and goats in seven provinces and one autonomous region of China. All blood samples were tested for hemoplasmas (M. ovis and ‘Candidatus M. haemovis’) by nested PCR amplification based on 16S rRNA gene. Positive specimens underwent nucleotide sequencing and phylogenetic analysis.ResultsOverall, 610 specimens (44.7%, 610/1364) were shown to be hemoplasmas (M. ovis and ‘Candidatus M. haemovis’) -positive by nested PCR amplification based on 16S rRNA gene. The prevalence in goats was 44.1% (379/860), and 45.8% (231/504) in sheep, while that in grazing small ruminants was 54.4% (396/728) and 33.6% (214/636) in house feeding small ruminants. Sequencing of the nearly complete 16S rRNA gene was successful for the 103 randomly selected positive specimens from different farms in different sampling sites of China. Among them, analysis of the 16S rRNA gene sequences identified M. ovis (n = 56) and ‘Candidatus M. haemovis’ (n = 47). Two (KU983740 and KU983746) of the four novel genotypes obtained in this study were closely related to M. ovis, while the other two genotypes (KU983748 and KU983749) had high identity with ‘Candidatus M. haemovis’. Remarkably, the genotype (KU983740) of M. ovis in sheep and goats in this study fell in a clade with two human hemoplasmas from USA (KF313922 and GU230144) and shared 99.8%–99.9% with them.ConclusionsIn this study, ‘Candidatus M. haemovis’ was first detected in Chinese sheep and goats and hemoplasmas (M. ovis and ‘Candidatus M. haemovis’) are highly prevalent, and widely distributed in China.Electronic supplementary materialThe online version of this article (doi:10.1186/s12917-017-1062-z) contains supplementary material, which is available to authorized users.

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A review of the occurrence of hemoplasmas (hemotrophic mycoplasmas) in Brazil

Cited by 65 publications

References 27 publications

Diagnóstico molecular da infecção por hemoplasmas em gatos domésticos naturalmente infectados da cidade de Belém, Pará

Diagnóstico molecular da infecção por hemoplasmas em gatos domésticos naturalmente infectados da cidade de Belém, Pará

Infection with Hemotropic Mycoplasma Species in Patients with or without Extensive Arthropod or Animal Contact

Molecular characterization of hemotropic mycoplasmas (Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’) in sheep and goats in China

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