Soroprevalência de Anaplasma marginale em bovinos da região Centro-Sul do estado do Paraná, Brasil, por um teste imunoenzimático competitivo utilizando proteína recombinante MSP5-PR1

Abstract

ResumoFoi determinada a prevalência de Anaplasma marginale em 223 soros de bovinos com idade maior ou igual a dois anos, das regiões de Ponta Grossa, Guarapuava e Laranjeiras do Sul, região Centro-Sul do estado do Paraná. O teste imunoenzimático de competição PR1 (cELISA-PR1) foi utilizado para determinar a presença ou ausência de anticorpos anti-A. marginale. Dos 223 soros analisados, 130 (58,74%) foram positivos pelo cELISA-PR1, sugerindo ser essa região de instabilidade de enzoótica, com uma porcentagem significativa de animais susceptíveis à infecção por A. marginale, com risco potencial para desenvolver a anaplasmose. As características de tipo de exploração da propriedade, sistema de criação, manejo e forma de comercialização dos animais foram avaliadas. A análise estatística não demonstrou haver diferença significativa entre as variáveis estudadas e a positividade dos animais.Palavras-chave: Anaplasma marginale, MSP5-PR1, cELISA, soroprevalência, bovino. AbstractAnaplasma marginale prevalence was determined in 223 sera samples in 2-year old or older cattle, from the CenterSouthern Region of the Paraná State, including Ponta Grossa, Guarapuava and Laranjeiras do Sul municipalities. A survey of antibodies IgG class against Anaplasma marginale was performed through a competitive immune absorbent assay (cELISA-PR1). From the 223 sera examined, 130 (58.74%) reacted to cELISA-PR1 test, suggesting an region of enzootic instability, with a significant percentage of animals susceptible to infection by A. marginale and potentially in risk to develop anaplasmosis. The kind of exploration in the property, the breeding and handling system, the presence of other animals (ovine/caprine, horses, wild animals), and means of commercialization of animals were analyzed. The statistical analysis showed that there were no significant differences among the analyzed variables.

Seroprevalence and risk factors for cattle anaplasmosis, babesiosis, and trypanosomiasis in a Brazilian semiarid region

“…microplus throughout the year, such as in southern Brazil (and Uruguay), where mean temperatures below 15 °C inhibit tick development during the autumn-winter months. Consequently, many animals do not come into contact with infected ticks during their first months, and when they first become infected later on, severe disease with high lethality is a common feature (NARI; SOLARI, 1991;MARTINS et al, 1994;GUGLIELMONE, 1995;MARANA et al, 2009).…”

Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil

“…The diagnosis consists of indirect detection of antibodies against the agents of the BBA complex. Several serological tests are used for detection of bovine babesiosis and anaplasmosis, and the following stand out among them: the rapid conglutination test (RCT), latex agglutination, hemagglutination, complement fixation (CF) (MARANA et al, 2006), test card (TC), indirect immunofluorescence assay (IFA) (SOUZA et al, 2001;MARANA et al, 2009), indirect enzymelinked immunosorbent assay (iELISA) and competitive ELISA (cELISA) (MARANA et al, 2009).…”

Enzootic instability for bovine anaplasmosis on family farms located in southwestern Paraná, Brazil

“…In the state of Bahia, northeastern Brazilian territory, a seropositivity of 94.8% was reported in cattle (BARROS et al, 2005). In other municipalities in the State of Paraná, antibodies anti-A.marginale were observed in 92.94% of cattle from Londrina (ANDRADE et al, 2001), 76.1% in Umuarama (YOSHIHARA et al, 2003) and 58.74% in Ponta Grossa, Guarapuava and Laranjeiras do Sul cities (MARANA et al, 2009).…”