Evaluation of Bone Repair in the Mandible of Rabbits Using Biphasic Calcium Phosphate Micro-Macroporous Hydroxyapatite Bioceramics and Beta-Tricalcium Phosphate

Abstract

Objective: To perform a clinical and histological evaluation, characterizing and proving the feasibility of the use of beta tricalcium phosphate (HA/βTCP) bioceramics as a bone defect repair material, comparing it with autogenous bone and blood clot in terms of osteoinductive, conductive, and genic capacities. Material and Methods: The experiment was based on 3 critical defects in the mandible of 11 New Zealand rabbits. The defects were filled with HA/βTCP bioceramics and autogenous bone, respectively, collected and ground during the development of defects and blood clots. The animals were euthanized after the 90-day experiment and samples were collected for histomorphological examination. To evaluate differences between the groups, a one-way analysis of variance (ANOVA) was performed with Tukey's post hoc test. An α value lower than 0.05 was considered statistically significant. Results: Microscopy revealed the presence of osteoblasts, osteoclasts, and osteocytes associated or not associated with the presence of mature or immature bone. All the studied materials presented bone neoformation in all cases, with the presence of mature and immature bone. Regarding the presence of HA/βTCP bioceramic residual material, the same was found in 7 of 11 slides. Conclusion: HA/βTCP bioceramics were shown to be a biocompatible bone substitute, with osteoinductive and osteoconductive characteristics, accelerating the process of new bone formation when compared with autogenous and blood clotted bone, thereby showing promise for bone defect repair with safety and efficacy.

Evaluation of autogenous tooth graft in regeneration of periodontal vertical alveolar defects: clinical, radiographic, and experimental study

“…Fatma Elsherbini, Mohamed Abdulrahman, Mohamed Mostafa El-Shennawi sites, which could be attributed to β-TCP's behavior by acting as a scaffold, on which new bone grew. Franceschini et al [43] agreed with the above by studying the repair of bony defects using β-TCP. They reported improved results.…”

Bioactive Inorganic Materials for Dental Applications: A Narrative Review

“…They form close contact with the tissue and the graft [ 244 ]. The inorganic bioactive materials, such as calcium silicate, hydroxyapatite, bioactive glass, amorphous calcium phosphate, etc., have successfully restored maxillo-facial bone defect after root-end resection and removal of granulation tissue [ 245 , 246 , 247 ]. In a clinical trial study [ 248 ], carbonated hydroxyapatite and Bio-Oss ® —Geistlich Biomaterials, Wolhuser, Switzerland) were placed after extraction, and a histological analysis of bone samples was completed after 90 days.…”

Effectiveness of biomechanically stable pergola-like additively manufactured scaffold for extraskeletal vertical bone augmentation

“…Unfortunately, the current 3D-printed scaffolds for bone augmentation are ordinary bone defect repair scaffolds, and there is no specific design for the osteogenic characteristics of bone augmentation. The existing research has shown that the osteogenesis of vertical bone augmentation is often associated with an increase in pore diameter ( Franceschini Neto et al, 2019 ; Ghayor et al, 2021 ). More importantly, it has been observed that the optimal microarchitecture for bone augmentation differs from the optimal one for a general bone defect repair ( Jiang et al, 2018 ; Vaquette et al, 2021b ).…”