Determination of PPD in Hair Dyes Collected from Local Markets in El-Bieda City - Libya

Abstract

This study aims to determine the para-phenylenediamine (PPD) in hair dyes by High-Performance Liquid Chromatography (HPLC). In the presented study, ten hair dye samples were collected from local markets in El-Bieda - Libya. A rapid, simple and reliable method is developed and validated for the determination of PPD in hair dyes samples using 50% methanol solution as solvent. The method was validated over a wide linear range of 5 – 25 µg/mL with correlation coefficients being consistently greater than 0.997. The LOD and LOQ were 1.21 µg/ ml and 3.67 µg/ ml, respectively. Relative standard deviation (% R.S.D.) ranged between 0.07 and 1.15 %. The minimum PPD level was observed in Beauty Touch (Blonde) sample (0.0855 % w/w), while Jourin sense Cosmetics (Black Blue) sample showed the highest PPD content (2.2526 % w/w). The HPLC measurments indicated that the results of PPD concentrations in Black colour samples were in the range of 0.3705 % - 2.2526 % w/w. Whereas its concentrations in anthor colour samples were in the range of 0.0855 % - 0.5936 % w/w. The level of PPD in Black colour samples were higher than other colour samples. The PPD content in all the samples analyzed in this study are well below the allowable limits set by the US Food and Drugs Administration.

Electrochemical approach for recognition and quantification ofp-phenylenediamine: a review

“…The method analyzed a low-level of p -PDA in the samples as given by the US Food and Drugs Administration. 52…”

“…The method analyzed a low-level of p-PDA in the samples as given by the US Food and Drugs Administration. 52 Sample preparation and chromatographic separation are common bottlenecks in analyses since these steps are often tedious, time-consuming and expensive. 53…”