Benzocaine and eugenol as anesthetics for Brycon hilarii

Fabiani, Bruno Matucita; Boscolo, Wilson Rogério; Feiden, Aldi; Diemer, Odair; Bittencourt, Fábio; Neu, Dacley Hertes

doi:10.4025/actascianimsci.v35i2.16644

Cited by 11 publications

(6 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Its anesthetic effects are desirable for teleost fish due to fast induction, short half-life, and rapid recovery time [16]. For these reasons, BENZ has been extensively used in fish production [17]. Despite the efficacy in fish, BENZ presents low bioavailability, resulting in weak and erratic absorption after immersion [18,19].…”

Section: Introductionmentioning

confidence: 99%

Potential of mucoadhesive nanocapsules in drug release and toxicology in zebrafish

et al. 2020

View full text Add to dashboard Cite

Mucoadhesive polymeric nanocapsules have attracted interest of researchers from different fields from natural sciences because of their ability to interact with the mucosa and increase drug permeation. Anesthesia by immersion causes absorption through the skin and gills of fish, so it is important to evaluate the exposure of these organs to drug nanosystems. Benzocaine (BENZ) is one of the most popular anesthetic agents used in fish anesthesia, but it has drawbacks because of its low bioavailability, resulting in weak absorption after immersion. Here we describe method developed for preparing and characterizing chitosan-coated PLGA mucoadhesive nanoparticles containing BENZ (NPMAs) for zebrafish immersion anesthesia. We determined the lowest effective concentration, characterized the interaction of the mucoadhesive system with fish, measured the anesthetic efficacy, and evaluated possible toxic effects in embryos and adults exposed to the nanoformulations. This study opens perspectives for using nanoformulations prepared with BENZ in aquaculture, allowing reduction of dosage as well as promoting more effective anesthesia and improved interaction with the mucoadhesive system of fish.

show abstract

Section: Introductionmentioning

confidence: 99%

Potential of mucoadhesive nanocapsules in drug release and toxicology in zebrafish

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Weight and length were monthly and individually measured. The fish were anaesthetized with 0.1% benzocaine (Fabiani et al, ) and killed according to the institutional animal care protocols and approval (375/2011—CEUA—IBB/UNESP). The dissected gonads were fixed by immersion in different solutions according to the different methodologies.…”

Section: Methodsmentioning

confidence: 99%

Reproductive indicators during the male sexual maturation of Steindachneridion melanodermatum (Teleostei: Siluriformes: Pimelodidae) in captivity

et al. 2019

View full text Add to dashboard Cite

The reproductive dynamics of a species is important information to have for the maintenance and reproduction of animals in captivity, for either commercial or conservation purposes. Steindachneridion melanodermatum is an endemic fish that has been reproduced by breeding protocols, but knowledge related to the maturation and reproductive cycle of this species is scarce. The purpose of this research was to observe different parameters related to the reproductive biology of males born in captivity over a 24-month period to determine the first maturation and reproductive activity of S. melanodermatum males maintained in captivity. Body development changes (weight and length) were observed, and gonadosomatic index (GSI) and plasma sex steroids (testosterone and 17β-estradiol) were measured monthly. Phases of the reproductive cycle were detected based on macroscopic changes and histological observations of the testes. The obtained data were correlated, demonstrating that the GSI values accompanied testis development and sex steroid peaks. Additionally, immunohistochemistry was performed for the detection of KI-67, 3β-HSD and Vasa protein. Our data analysis indicated that the first maturity in males bred in captivity occurred after one year (at 22-month-old fish), with a reproductive period occurring from September to November, which is the spring season in the southern hemisphere. K E Y W O R D Sfirst maturation, sex steroids, sperm morphology, Surubim do Iguaçu, testis histology

show abstract

“…The most widely used natural anesthetic is eugenol (4-allyl-2-methoxyphenol), the main compound from clove oil, which is obtained from plants of the genus Eugenia (ANDERSON et al, 1997). The anesthetic efficacy of eugenol was showed for several Neotropical species as Piaractus mesopotamicus (Holmberg, 1887) (GONÇALVES et al, 2008), Astyanax bimaculatus (Linnaeus, 1758) (SILVA et al, 2009), Brycon amazonicus (Spix & Agassiz, 1829) (VIDAL et al, 2007), Rhamdia quelen (Quoy & Gaimard, 1824) (CUNHA et al, 2010a), Carassius auratus (Linnaeus, 1758) (BITTENCOURT et al, 2012), Centropomus parallelus Poey, 1860(SOUZA et al, 2012 and Brycon hilarii (Valenciennes, 1850) (FABIANI et al, 2013). The efficacy of the essential oil (EO) of Lippia alba was also demonstrated for Rhamdia quelen (CUNHA et al, 2010b;HELDWEIN et al, 2012HELDWEIN et al, , 2014TONI et al, 2014), Hippocampus reidi (Ginsburg, 1933) (CUNHA et al, 2011) and Sparus aurata (Linnaeus, 1758) (TONI et al, 2015), but the EO of Aloysia triphylla was studied only in R. quelen (PARODI et al, 2014) and C. parallelus (PARODI et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Óleos essenciais e eugenol como anestésico para Serrasalmus rhombeus

et al. 2018

View full text Add to dashboard Cite

This study evaluated the periods of time of anesthetic induction and recovery of Serrasalmus rhombeus exposed to essential oils (EOs) of Aloysia triphylla and Lippia alba and eugenol, as well as if these anesthetics can be used for transport of this species, through analysis of swimming behavior. Fish were placed in aquaria containing different concentrations of A. triphylla EO or L. alba EO or eugenol, and posteriorly were transferred to aquaria containing only water to evaluate the recovery time. In the second experiment, behavior was analyzed during exposure to A. triphylla EO, L. alba EO or eugenol at 5 or 10 µL L-1. The evaluations were carried out at 0, 1, 5, 10 and 15 min of exposure. Fish exposed to 150, 200 and 50 µL L-1 of A. triphylla EO, L. alba EO and eugenol, respectively, showed anesthetic induction time lower than 3 min and recovery time lower than 10 min. Concentrations of 50 µL L-1 of both EOs and 25 µL L-1 eugenol caused only sedation. Exposure to 5 and 10 µL L-1 EOs and eugenol decreased fish swimming time. Both EOs and eugenol were effective for anesthesia and can be used for transport of S. rhombeus.

show abstract

Benzocaine and eugenol as anesthetics for Brycon hilarii

Cited by 11 publications

References 17 publications

Potential of mucoadhesive nanocapsules in drug release and toxicology in zebrafish

Potential of mucoadhesive nanocapsules in drug release and toxicology in zebrafish

Reproductive indicators during the male sexual maturation of Steindachneridion melanodermatum (Teleostei: Siluriformes: Pimelodidae) in captivity

Óleos essenciais e eugenol como anestésico para Serrasalmus rhombeus

Contact Info

Product

Resources

About