<b>Cultivation of immature <i>Capsicum</i> spp. embryos for incompatible-crossing embryo rescue

Abstract

The in vitro culture of embryos is an important technique to enable the rescue of embryos from incompatible crosses. Studies analyzing the factors that affect the in vitro culture of zygotic embryos are scarce. This study evaluated the effect of the genotype (Capsicum baccatum and C. frutescens), the composition of the culture medium (MS and ½ MS medium with different concentrations of sucrose, IAA and GA 3 ) and the stage of development (globular, cordiform, torpedo and cotyledonary) in the in vitro culture of immature embryos. Embryo germination was influenced primarily by the stage of development of the embryo and the composition of the culture medium. Regardless of the species, the most suitable culture medium for the germination of globular and cordiform embryos was ½ MS with 0.05 mg L -1 of GA 3 and IAA and 40 g L -1 of sucrose. For torpedo and cotyledonary embryos, ½ MS culture medium with 20 g L -1 of sucrose without phytoregulators is recommended for germination. These results were better than those described in the literature for all development stages in the two species. The results in the present study will be useful for geneticists and genetic enhancers interested in applying germination techniques conducted in vitro for immature Capsicum embryos.

Biotive protease inhibitor from leaves of Capsicum annuum L.: Potential use as an antifungal molecule against Colletotrichum scovillei

“…The cultivation was carried out in Petri dishes (90  15 mm) containing MS medium (½ MS), white vitamin complex 20 and 100 mg L À1 myo-inositol, with pH 5.7 ± 0.1 and, as a solidifying agent, 2 g L À1 Phytagel (Sigma) was used after 15 days the plants were transferred to 350 mL glass flasks (125 mm  60 mm) with the same culture medium and 20 g L À1 sucrose were added. The plants were kept in a growth room for 30 days at 27 ± 2 C under a light: dark photoperiod of 16 h with an irradiance of 50 μmol m À2 s À1 through the use of OSRAM ® daylight fluorescent lamps, 21 at the Laborat orio de Fitotecnia, UENF, RJ, Brazil,…”