2014
DOI: 10.4025/actasciagron.v36i2.17346
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Abstract: The aim of this study was to evaluate the storage potential of the pollen grains of Brazil Green Dwarf (BGD), Brazilian Tall (BRA) and Cameroon Red Dwarf (CRD) coconut accessions under different storage conditions. Representative plants of BGD, BRA and CRD accessions were selected from the Coconut Active Germplasm Bank of Embrapa Coastal Tablelands. The pollen grains were collected, placed in cryotubes and maintained under the following storage conditions: T1, refrigerator (-4°C); T2, freezer (-20°C); T3, ultr… Show more

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Cited by 23 publications
(14 citation statements)
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“…For a successful hybridization program, a reliable method of pollen storage is required. Under controlled temperature and storage humidity this method has been successfully applied to a wide range of angiosperms such as almond (Martinez-gomez et al, 2000;, Aechmea fasciata, A. chantinii, Vriesea splendens, Guzmania lingulata and Tillanproadsia cyanea (Parton et al, 2002), Melaleuca alternifolia (Baskorowati, 2009), Cocos nucifera (Machado et al, 2014), Imperata cylindrica (Rather et al, 2017) and Rosa hybrida (Giovannini et al, 2017). The preservation of S. formosa pollen was first reported by Hughes et al (1991).…”
Section: Introductionmentioning
confidence: 99%
“…For a successful hybridization program, a reliable method of pollen storage is required. Under controlled temperature and storage humidity this method has been successfully applied to a wide range of angiosperms such as almond (Martinez-gomez et al, 2000;, Aechmea fasciata, A. chantinii, Vriesea splendens, Guzmania lingulata and Tillanproadsia cyanea (Parton et al, 2002), Melaleuca alternifolia (Baskorowati, 2009), Cocos nucifera (Machado et al, 2014), Imperata cylindrica (Rather et al, 2017) and Rosa hybrida (Giovannini et al, 2017). The preservation of S. formosa pollen was first reported by Hughes et al (1991).…”
Section: Introductionmentioning
confidence: 99%
“…According to that report, 38.54% germination was observed from the medium containing 8% sucrose, 1% gelatin, 1% agar and 0.01% boric acid (Karun et al, 2006). In addition, research carried out by Machado et al in Brazil to assesses the pollen grain viability of coconut accessions at low temperatures under different storage conditions [refrigerator (-4ºC); freezer (-20ºC); ultra-freezer (-80ºC) and liquid nitrogen (-196ºC)] has reported that pollen of Brazilian Tall (BRA) shows a significant effect on storage conditions and storage times (Machado et al, 2014). A higher viability for pollen germination (81.93%) upon storage in liquid nitrogen has been reported in this study.…”
Section: Pollenmentioning
confidence: 99%
“…First studies with coconut were carried out by ASSY-BAH and ENGELMANN (1992), using vitrification technique in mature PB 121 zygotic hybrid coconut embryos (Malayan yellow dwarf x African tall), Cameroon red dwarf, Indian tall and Rennel tall coconut genotypes. Several researchers have published promising results with different techniques using zygotic embryos, plumules, inflorescence and pollen as explants (SAJINI et al, 2011;CUETO et al, 2014;MACHADO et al, 2014;WELEWANNI et al, 2017).…”
Section: Introductionmentioning
confidence: 99%