Estresse hídrico com diferentes osmóticos em sementes de feijão e expressão diferencial de proteínas durante a germinação

Coelho, Danielle Letícia Martins; Agostini, Edna Antônia Torquato de; Guaberto, Luciana Machado; Neto, Nelson Barbosa Machado; Custódio, Ceci Castilho

doi:10.4025/actasciagron.v32i3.4694

Cited by 13 publications

(5 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A special group of HSPs, the chaperones, has also been found in this molecular weight range and are involved in maintaining protein homeostasis, recovering damaged proteins, preventing, maintaining or renaturing denatured proteins (Dahiya & Buchner, 2019), important functions in cells that have been subjected to thermal stresses (Borges & Ramos, 2005). Other authors related the expression of bands in the 65, 45, 25 and 18 kDa ranges to responses to other stress types, such as the water stress (Coelho et al, 2010a).…”

Section: /7mentioning

confidence: 99%

Resistance to degradation of reserve proteins in beans after hydration and cooking

Araldi

Coelho

Shibata

2020

Agraria

View full text Add to dashboard Cite

Hydration and cooking of beans leads to significant losses in their nutritional quality, but some genotypes may have a greater resistance to protein degradation. Therefore, this study aimed to evaluate the effect of the genotype on both quality and quantity of proteins available in the beans before and after hydration and cooking. The grains of six landrace genotypes from the Active Bean Germplasm Bank of the UDESC (BAFs: 03, 07, 57, 75, 97 and 102) were used "in natura" (control) and subjected to the following treatments: hydration for 8 hours and hydration followed by cooking. The content and electrophoretic profile of the soluble proteins were evaluated. Initially, the protein content ranged from 39.4 (BAF 57) to 51.1 mg g-1 FW (BAF 75). Hydration provoked a decrease in the protein content for all genotypes, having it been accentuated with the cooking. BAFs 07 and 75 presented a higher protein content after cooking, 29.1 and 31.4 mg g-1 FW, respectively, and concomitantly, higher intensity and presence of protein bands. In conclusion, BAFs 07 and 75 demonstrate a higher resistance to protein degradation and their use may be recommended in crosses aimed at improving the nutritional and technological grain quality.

show abstract

Section: /7mentioning

confidence: 99%

Resistance to degradation of reserve proteins in beans after hydration and cooking

Araldi

Coelho

Shibata

2020

Agraria

View full text Add to dashboard Cite

show abstract

“…Reduction in the osmotic potential to -0.4 MPa drastically affected radicle development in cowpea seedlings, causing declines of 61.66 and 66.85% in root length and root dry matter accumulation, respectively ( Figures 1B and D). In the germination of Phaseolus vulgaris under water stress, Coelho et al (2010), Garcia et al (2012) and Carvalho et al (2013) observed that the reduction in osmotic potential substantially reduced root development and, consequently, phytomass accumulation. These authors attributed this effect to the decrease in seed hydration, since water constitutes the matrix for the occurrence of biochemical and physiological processes, which culminate in radicle protrusion.…”

Section: Dmentioning

confidence: 99%

Germination and tolerance of cowpea (Vigna unguiculata) cultivars to water stress

Paiva

Sá

Torres

et al. 2018

Rev. bras. eng. agríc. ambient.

View full text Add to dashboard Cite

The objective of this study was to evaluate the tolerance of cowpea cultivars to water stress, during seed germination and seedling establishment. For this, a completely randomized experimental design was used in a factorial scheme consisting of nine cultivars of cowpea (BRS Guariba, BRS Potengi, BRS Itaim, BRS 17 Gurguéia, BRS Aracê, Paulistinha, Maratanã, Costela-de-Vaca and Canapu-Branco) and two levels of osmotic potential induced by PEG 6000 (0.0 MPa (control) and -0.4 MPa) in four replicates of 50 seeds. The variables analysed were germination, germination first count, root and shoot lengths, and dry matter accumulation of shoots and roots. The dissimilarity between the genotypes was also determined by Euclidean distance. Germination and initial development of cowpea cultivars are impaired by the reduction in the potential to -0.4 MPa. The cultivars BRS Guariba, BRS Aracê, Paulistinha and Canapu-Branco are the most tolerant to water stress, while BRS Potengi, BRS Itaim, BRS 17 Gurguéia, BRS Maratanã and Costela-de-Vaca are the most sensitive in the early development stage.

show abstract

“…A disponibilidade hídrica e o movimento da água para as sementes são importantes para a germinação e emergência das plântulas, e desse modo, são fatores influenciados pelo potencial hídrico do solo (KAPPES et al, 2009). Nem sempre o solo oferece um ambiente ótimo para a germinação das sementes e uma das condições por vezes encontrada é a pouca disponibilidade de água logo após a semeadura (Coelho et al, 2010). Nessa condição, o solo apresenta potenciais hídricos que dificultam a absorção da água necessária para a germinação das sementes (MACHADO NETO et al, 2006), o que configura o estresse hídrico logo após o plantio como um dos fatores abióticos que mais influência no processo germinativo, interferindo na atividade enzimática da planta (GARCIA et al, 2012).…”

Section: Introductionunclassified

Déficit Hídrico E a Germinação De Sementes De Híbridos De Milho

Henrique

Bosqueiro

Kotsubo

et al. 2021

NAT

View full text Add to dashboard Cite

Considerando a importância da água no processo de germinação de sementes, as condições adversas de umidade afetam diretamente o processo germinativo. Para tanto, objetivou-se avaliar a influência do deficit hídrico na germinação e vigor de sementes de dois genótipos comerciais de milho, induzido por diferentes potenciais osmóticos. Utilizou-se delineamento experimental inteiramente casualizados (DIC), arranjado em esquema fatorial duplo 2x5, com quatro repetições, avaliando híbridos de milho (DKB255PRO3 e DKB390PRO2), submetidos a germinação sob condições de déficit hídrico, em cinco níveis de potencial osmótico (0, -0,6, -1,2, -1,8 e -2,4 MPa). Cada unidade experimental constituiu-se por um rolo de papel germitest contendo 50 sementes. Utilizou-se NaCl como agente osmótico indutor de deficiência hídrica as sementes. Avaliou-se a germinação, vigor de sementes e o desenvolvimento inicial das plântulas, em termos de comprimento e biomassa. O déficit hídrico, simulado com solução de cloreto de sódio, provoca redução no desempenho de sementes de híbridos de milho. Os genótipos de milho respondem diferentemente quanto a tolerância a condições de estresse provocado por indisponibilidade de água durante a germinação. O híbrido DKB390PRO2 apresenta germinação e crescimento de plântulas superior quando submetido a diferentes níveis de potencial osmótico de NaCl. Palavras-chave: viabilidade de sementes; restrição hídrica; Zea mays. Effect of water deficit on the maize seed germination ABSTRACT: Considering the importance of water in the seed germination process and knowing that conditions will not always be the most adequate to the germination process, the objective was to evaluate the influence of water deficit on germination and vigor in seeds of different commercial corn genotypes induced by different osmotic potentials. A completely randomized experimental design (D.I.C) was used, arranged in a 2x5 double factorial scheme, with four replications. Each experimental unit was constituted by a roll containing 50 seeds, made in germitest paper. Two maize seed hybrids (DKB255PRO3 and DKB390PRO2) were evaluated, submitted to germination under water deficit conditions, with levels 0; -0,6. -1,2; -1,8 and -2,4 MPa of osmotic potential. NaCl was used as osmotic agent inducing water deficiency in seeds. Germination, seed vigor and initial seedling development were evaluated in terms of length and biomass. The water deficit simulated with sodium chloride solution, causes a reduction in the performance of maize hybrids seeds. Corn genotypes respond differently to tolerance to stress conditions caused by unavailability of water during germination. The hybrid DKB390PRO2 presents superior germination and seedling growth when submitted to different levels of osmotic NaCl potential. Keywords: viability; water restriction; Zea mays.

show abstract

Estresse hídrico com diferentes osmóticos em sementes de feijão e expressão diferencial de proteínas durante a germinação

Cited by 13 publications

References 38 publications

Resistance to degradation of reserve proteins in beans after hydration and cooking

Resistance to degradation of reserve proteins in beans after hydration and cooking

Germination and tolerance of cowpea (Vigna unguiculata) cultivars to water stress

Déficit Hídrico E a Germinação De Sementes De Híbridos De Milho

Contact Info

Product

Resources

About