Peroxiredoxin 3 is a novel marker for cell proliferation in cervical cancer

Abstract

Abstract. Although peroxiredoxin 3 (Prx3) has been reported to be involved in cervical cancer (CC) carcinogenesis, the significance of Prx3 in CC progression remains unclear. The present study was conducted to investigate the expression features of Prx3 to better understand the mechanism of tumor growth and invasion. Sixty-eight patients with invasive squamous cervical cancer were included in the present study. The status of human papillomavirus (HPV) infection was detected by hybridization and quantitative real-time polymerase chain reaction (qRT-PCR). Immunohistochemistry was performed on paraffin-embedded sections using monoclonal antibodies against Prx3 and Ki67. All samples were positive for high-risk HPV, among which fifty-six samples were positive for HPV16, seven for HPV18 and five for HPV33. The expression of HPV16 E6/E7 was significantly higher in cancer areas compared to the adjacent normal epithelial tissuses. The positive cells for Prx3 and Ki67 were significantly higher in cancer cells compared to normal epitheliums and the staining pattern of Prx3 was consistent with that of Ki67 (Pearson's correlation coefficient was 0.801, P= 0.000). The upregulation of Prx3 might be a protective response to oxidative stress in the cancer microenvironment. The expression consistency of Prx3 and Ki67 suggests Prx3 to be a potential marker for cell proliferation of CC.

The functional role of peroxiredoxin 3 in reactive oxygen species, apoptosis, and chemoresistance of cancer cells

“…Notably, PRX3 was prominently up-regulated in nearly all the cancers except MM, in which the PRX3 positivity (36 % of all detected cases) was less than that of PRX1 (69 %), PRX2 (75 %), PRX5 (67 %), and PRX6 (83 %) (Kinnula et al 2002). In addition to the association of PRX3 expression with cell growth in breast and cervical cancers, respectively (Chua et al 2010;Hu et al 2013), the pattern of PRX3 immunostaining showed correlations with clinicopathological variables of some cancers. For example, the immunohistochemical expression of PRX3 was positively associated with tumor stage and prostate-specific antigen level in prostate cancer (Basu et al 2011).…”

“…For example, the immunohistochemical expression of PRX3 was positively associated with tumor stage and prostate-specific antigen level in prostate cancer (Basu et al 2011). PRX3 over-expression was correlated with poor cell differentiation of breast cancer, hepatocellular carcinoma, and cervical cancer (Karihtala et al 2003;Qiao et al 2012;Hu et al 2013). These observations make PRX3 a promising candidate for cell proliferation marker and a potential prognostic biomarker for cancers.…”

“…As an active responder to oxidative stress, PRX3 (or together with PRX5, another mitochondrial PRX gene) was significantly up-regulated in most common malignancies including breast cancer (Noh et al 2001;Karihtala et al 2003;Chua et al 2010), hepatocellular carcinoma (Choi et al 2002;Qiao et al 2012), malignant mesothelioma (MM) (Kinnula et al 2002), lung cancer Park et al 2006), cervical cancer (Kim et al 2009;Hu et al 2013), colorectal neoplasm (Wu et al 2010), prostate cancer (Basu et al 2011;Whitaker et al 2013), and endometrial cancer .…”

The sulfiredoxin–peroxiredoxin (Srx–Prx) axis in cell signal transduction and cancer development

“…In medulloblastoma, Prx3 is a target of MiR-383 (a microRNA), and its expression reduces cell proliferation [114]. In cervical cancer, Prx3 is over-expressed and its levels are correlated with increased rate of cell proliferation [115]. SNP RS7082598 of PRDX3 gene is correlated with a reduced risk of cervical cancer [116].…”

Epigenetic regulation of peroxiredoxins: Implications in the pathogenesis of cancer

“…46 Moreover, PRX III was also proposed to function as a marker for cellular proliferation in breast cancer and ....................................................................................................................... cervical cancer. 22,47 Furthermore, PRXs expression could potentially be a marker for radiation responsiveness, since elevated PRXs levels can be frequently observed in resistant cells. This allows for more precise selection of suitable patients that would benefit immensely from the therapeutic regimes to be instituted.…”