Bioactive and Bioadhesive Catechol Conjugated Polymers for Tissue Regeneration

Puertas-Bartolomé, María; Vázquez, Blanca; Román, Julio San

doi:10.3390/polym10070768

Cited by 21 publications

(16 citation statements)

References 125 publications

(164 reference statements)

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“…The requirement for iron in enterobactin trimer-mediated peroxide detoxification is currently unknown. The second potential mechanism for Ent-TRI detoxification of reactive oxygen species is based on the well-known antioxidant activity of catechol moieties (64)(65)(66)(67)(68). These moieties are present and exposed that in the linearized enterobactin trimer.…”

Section: Discussionmentioning

confidence: 99%

Linearized Siderophore Products Secreted via MacAB Efflux Pump Protect Salmonella enterica Serovar Typhimurium from Oxidative Stress

Bogomolnaya

Tilvawala

Elfenbein

et al. 2020

mBio

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Nontyphoidal salmonellae (NTS) are exposed to reactive oxygen species (ROS) during their residency in the gut. To survive oxidative stress encountered during infection, salmonellae employ several mechanisms. One of these mechanisms involves the multidrug efflux pump MacAB, although the natural substrate of this pump has not been identified. MacAB homologs in pseudomonads secrete products of nonribosomal peptide synthesis (NRPS). In Salmonella enterica serovar Typhimurium, the siderophore enterobactin is produced by NRPS in response to iron starvation and this molecule can be processed into salmochelin and several linear metabolites. We found that Salmonella mutants lacking the key NRPS enzyme EntF are sensitive to peroxide mediated killing and cannot detoxify extracellular H2O2. Moreover, EntF and MacAB function in a common pathway to promote survival of Salmonella during oxidative stress. We further demonstrated that S. Typhimurium secretes siderophores in iron-rich media when peroxide is present and that these MacAB-secreted metabolites participate in protection of bacteria against H2O2. We showed that secretion of anti-H2O2 molecules is independent of the presence of the known siderophore efflux pumps EntS and IroC, well-described efflux systems involved in secretion of enterobactin and salmochelin. Both salmochelin and enterobactin are dispensable for S. Typhimurium protection against ROS; however, linear metabolites of enterobactin produced by esterases IroE and Fes are needed for bacterial survival in peroxide-containing media. We determined that linearized enterobactin trimer protects S. Typhimurium against peroxide-mediated killing in a MacAB-dependent fashion. Thus, we suggest that linearized enterobactin trimer is a natural substrate of MacAB and that its purpose is to detoxify extracellular reactive oxygen species. IMPORTANCE Nontyphoidal Salmonella bacteria induce a classic inflammatory diarrhea by eliciting a large influx of neutrophils, producing a robust oxidative burst. Despite substantial progress understanding the benefits to the host of the inflammatory response to Salmonella, little is known regarding how Salmonella can simultaneously resist the damaging effects of the oxidative burst. The multidrug efflux pump MacAB is important for survival of oxidative stress both in vitro and during infection. We describe a new pathway used by Salmonella Typhimurium to detoxify extracellular reactive oxygen species using a multidrug efflux pump (MacAB) to secrete a linear siderophore, a metabolite of enterobactin. The natural substrates of many multidrug efflux pumps are unknown, and functional roles of the linear metabolites of enterobactin are unknown. We bring two novel discoveries together to highlight an important mechanism used by Salmonella to survive under the oxidative stress conditions that this organism encounters during the classic inflammatory diarrhea that it also induces.

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Section: Discussionmentioning

confidence: 99%

Linearized Siderophore Products Secreted via MacAB Efflux Pump Protect Salmonella enterica Serovar Typhimurium from Oxidative Stress

Bogomolnaya

Tilvawala

Elfenbein

et al. 2020

mBio

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“…The introduction of aromatic diols (catechols such as quercetin or its derivatives, catechins, dopamine, (hydro)caffeic acid, or the related gallic acid or gallol) on or in polymer backbones has been shown to confer antioxidant properties. In a somehow surprising fashion, these materials often exhibit very significant ROS‐ and in particular superoxide‐scavenging activity, also when obtained under conditions that should oxidize catechols (radical or oxidative polymerization, the latter also including enzymatic conversion to quinones): only in few cases care has been taken to protect their OH groups during preparative processes or at least to introduce them as the last step of a synthetic sequence . It must be said, however, that oxidized catechol products can also have antioxidant activity, as shown for melanins and melanin‐inspired compounds …”

Section: Oxidation (Ros)‐responsive Materialsmentioning

confidence: 99%

Oxidation‐Responsive Materials: Biological Rationale, State of the Art, Multiple Responsiveness, and Open Issues

El-Mohtadi

d’Arcy

Tirelli

2018

Macromol. Rapid Commun.

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In this review, a general introduction to biological oxidants (focusing on reactive oxygen species, ROS) and the biomedical rationale behind the development of materials capable of responding to ROS is provided. The state of the art for preparative aspects and mechanistic responses of the most commonly used macromolecular ROS‐responsive systems, including polysulfides, polyselenides, polythioketals, polyoxalates, and also oligoproline‐ and catechol‐based materials, is subsequently given. The endowment of multiple responsiveness, with specific emphasis on the cases where a molecular logic gate behavior can be obtained, is focused on. Finally, fundamental open issues, which include implications of the “drug”‐like character of ROS‐responsive materials (inherent anti‐inflammatory behavior) and the poor quantitative understanding of ROS roles in biology, are discussed.

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“…Compared to conventional wound dressings, such as gauze and films, bioadhesive hydrogels are biologically more compatible and can be removed more easily from the wound. The bioadhesive property can be provided to the hydrogel dressings by modification with polyphenol derived moieties, such as catechol, dopamine, gallic acid, or tannic acid [ 51 , 52 , 58 , 79 , 80 ].…”

Section: Functional Hydrogels For Chronic Wound Healingmentioning

confidence: 99%

Functional Hydrogels for Treatment of Chronic Wounds

Firlar

Altunbek

McCarthy

et al. 2022

Gels

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Chronic wounds severely affect 1–2% of the population in developed countries. It has been reported that nearly 6.5 million people in the United States suffer from at least one chronic wound in their lifetime. The treatment of chronic wounds is critical for maintaining the physical and mental well-being of patients and improving their quality of life. There are a host of methods for the treatment of chronic wounds, including debridement, hyperbaric oxygen therapy, ultrasound, and electromagnetic therapies, negative pressure wound therapy, skin grafts, and hydrogel dressings. Among these, hydrogel dressings represent a promising and viable choice because their tunable functional properties, such as biodegradability, adhesivity, and antimicrobial, anti-inflammatory, and pre-angiogenic bioactivities, can accelerate the healing of chronic wounds. This review summarizes the types of chronic wounds, phases of the healing process, and key therapeutic approaches. Hydrogel-based dressings are reviewed for their multifunctional properties and their advantages for the treatment of chronic wounds. Examples of commercially available hydrogel dressings are also provided to demonstrate their effectiveness over other types of wound dressings for chronic wound healing.

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Bioactive and Bioadhesive Catechol Conjugated Polymers for Tissue Regeneration

Cited by 21 publications

References 125 publications

Linearized Siderophore Products Secreted via MacAB Efflux Pump Protect Salmonella enterica Serovar Typhimurium from Oxidative Stress

Linearized Siderophore Products Secreted via MacAB Efflux Pump Protect Salmonella enterica Serovar Typhimurium from Oxidative Stress

Oxidation‐Responsive Materials: Biological Rationale, State of the Art, Multiple Responsiveness, and Open Issues

Functional Hydrogels for Treatment of Chronic Wounds

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