Effect of Methyl–β–Cyclodextrin and Trehalose on the Freeze–Drying and Spray–Drying of Sericin for Cosmetic Purposes

Abstract

Sericin is a protein extracted from Bombyx mori silk cocoons. Over the last decade, this wastewater product of the textile industry has shown many interesting biological properties. This protein is widely used in the cosmetic and biomedical fields. In this study, sericin has been obtained via a High–Temperature High–Pressure degumming process, and was dried using the freeze–drying (fd) and spray–drying (sd) techniques. Proteins tend to collapse during drying, hence, sericin has been dried in the presence of two selected carrier agents: methyl–β–cyclodextrin and trehalose. The obtained powders have been analyzed using thermal investigation, microscopy (optical, SEM), and granulometric and spectroscopic analyses. Moreover, the percentage yield of the spray–drying process has been calculated. Both the agents were able to significantly improve the drying process, without altering the physico–chemical properties of the protein. In particular, the co–spray–drying of sericin with methyl–β–cyclodextrin and trehalose gave good process yields and furnished a powder with low moisture content and handling properties that are better than those of the other studied dried products. These characteristics seem to be appropriate and fruitful for the manufacturing of cosmetic raw materials.

Nanoencapsulation as a General Solution for Lyophilization of Labile Substrates

“…The degradation temperatures were determined from the peak of derivative weight curves. For all the samples analyzed, there were two decomposition temperatures (Td) with the first one corresponding to the water loss and the second one linked to the degradation of the protein samples, as reported earlier (Figure 3h) [40]. The average moisture content in all the freeze-dried samples is represented in Table 1.…”

Evaluation of the Physicochemical and Functional Properties of Aquasoya (Glycine max Merr.) Powder for Vegan Muffin Preparation

“…Although the protein content of EWP was four times higher than that of YSP and CHP ( Table 1 ), there was no significant difference in EC ( p > 0.05). According to Giovannelli et al (2021), it was estimated that carrier agents were used during the spray-drying process of EWP [ 46 ]. As the concentration of carriers increased, it was expected that the depletion flocculation occurred, and the emulsifying properties decreased [ 47 ].…”

Optimization of spray-drying conditions for obtaining Bacillus sp. SMIA-2 protease powder

“…In fact, the culture supernatant generally contains very low amount of solids leading to high drying cost per unit weight of the product formed with very low product recoveries (Gupta et al, 2014). Therefore, the use of excipients is justified not only to avoid thermal denaturation leading to loss of enzyme activity (Silva et al, 2007;Piszkiewicz & Pielak, 2019;Giovannelli et al, 2021), but also to develop more stable enzyme formulations and to improve dry mass recovery purposes (Libardi et al, 2020).…”