Editorial: CRISPR-Cas in Agriculture: Opportunities and Challenges

Kumar, Sandeep; Rymarquis, Linda A.; Ezura, Hiroshi; Nekrasov, Vladimir

doi:10.3389/fpls.2021.672329

Cited by 12 publications

(4 citation statements)

References 17 publications

(13 reference statements)

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“…Many countries formally declare that crops will not be regulated under biosafety legislation if the product of the genome-edited crops do not contain foreign DNA [151]. Several countries including Brazil, Argentina, Japan, and the United States have already exempted genome-edited crops from being regulated in the same way as genetically modified organisms (GMOs) [152].…”

Section: Challenge and Future Prospectsmentioning

confidence: 99%

Research Trends and Challenges of Using CRISPR/Cas9 for Improving Rice Productivity

Kim

Jung

et al. 2022

Agronomy

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Nowadays, rice production faces significant challenges due to population pressure, global climate change, and outbreak of various pests and diseases. Breeding techniques used to improve rice traits include mutant breeding, cross breeding, heterogeneity, transformation, molecular markers, genome-wide association study (GWAS), and so on. Since the recently developed CRISPR/Cas9 technology can directly target a specific part of a desired gene to induce mutation, it can be used as a powerful means to expand genetic diversity of crops and develop new varieties. So far, CRISPR/Cas9 technology has been used for improving rice characteristics such as high yield, good quality, abundant nutrition, pest and disease resistance, herbicide resistance, and biotic and abiotic stress resistance. This review highlights the mechanisms and optimization of the CRISPR system and its application to rice crop, including resistance to biotic and abiotic stresses, and improved rice quality and yield.

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Section: Challenge and Future Prospectsmentioning

confidence: 99%

Research Trends and Challenges of Using CRISPR/Cas9 for Improving Rice Productivity

Kim

Jung

et al. 2022

Agronomy

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“…In vegetable breeding, in addition to genome breeding that makes full use of genome information and phenotyping technology, there is great potential for genome editing that has been developed in recent years. In countries with a product-based mindset, unlike traditional GM crops, GM technology is used to generate genome-edited crops, but null segregants do not contain transgenes, which can be suggested using Southern hybridization and PCR (El-Mounadi et al, 2020;Kumar S. et al, 2021). In this case, the deregulation process required for GM crops becomes unnecessary; thus, commercialization is relatively easy, and consumers' resistance to GM can be excepted.…”

Section: Developing Heat-tolerant Tomatoes For Breedingmentioning

confidence: 99%

Genetic and Molecular Mechanisms Conferring Heat Stress Tolerance in Tomato Plants

et al. 2021

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Climate change is a major threat to global food security. Changes in climate can directly impact food systems by reducing the production and genetic diversity of crops and their wild relatives, thereby restricting future options for breeding improved varieties and reducing the ability to adapt crops to future challenges. The global surface temperature is predicted to rise by an average of 0.3°C during the next decade, and the Paris Agreement (Paris Climate Accords) aims to limit global warming to below an average of 2°C, preferably to 1.5°C compared to pre-industrial levels. Even if the goal of the Paris Agreement can be met, the predicted rise in temperatures will increase the likelihood of extreme weather events, including heatwaves, making heat stress (HS) a major global abiotic stress factor for many crops. HS can have adverse effects on plant morphology, physiology, and biochemistry during all stages of vegetative and reproductive development. In fruiting vegetables, even moderate HS reduces fruit set and yields, and high temperatures may result in poor fruit quality. In this review, we emphasize the effects of abiotic stress, especially at high temperatures, on crop plants, such as tomatoes, touching upon key processes determining plant growth and yield. Specifically, we investigated the molecular mechanisms involved in HS tolerance and the challenges of developing heat-tolerant tomato varieties. Finally, we discuss a strategy for effectively improving the heat tolerance of vegetable crops.

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“…CRISPR/Cas is by far the most common plant genome editing technology nowadays due to its precision, versatility and ease of use [ 3 ]. It is an excellent tool for gene function studies as well as improvement of agriculturally important crop traits.…”

Section: Introductionmentioning

confidence: 99%

Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties

Hahn

Loures

Sparks

et al. 2021

Plants

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CRISPR/Cas technology has recently become the molecular tool of choice for gene function studies in plants as well as crop improvement. Wheat is a globally important staple crop with a well annotated genome and there is plenty of scope for improving its agriculturally important traits using genome editing technologies, such as CRISPR/Cas. As part of this study we targeted three different genes in hexaploid wheat Triticum aestivum: TaBAK1-2 in the spring cultivar Cadenza as well as Ta-eIF4E and Ta-eIF(iso)4E in winter cultivars Cezanne, Goncourt and Prevert. Primary transgenic lines carrying CRISPR/Cas-induced indels were successfully generated for all targeted genes. While BAK1 is an important regulator of plant immunity and development, Ta-eIF4E and Ta-eIF(iso)4E act as susceptibility (S) factors required for plant viruses from the Potyviridae family to complete their life cycle. We anticipate the resultant homozygous tabak1-2 mutant lines will facilitate studies on the involvement of BAK1 in immune responses in wheat, while ta-eif4e and ta-eif(iso)4e mutant lines have the potential to become a source of resistance to wheat spindle streak mosaic virus (WSSMV) and wheat yellow mosaic virus (WYMV), both of which are important pathogens of wheat. As winter wheat varieties are generally less amenable to genetic transformation, the successful experimental methodology for transformation and genome editing in winter wheat presented in this study will be of interest to the research community working with this crop.

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Editorial: CRISPR-Cas in Agriculture: Opportunities and Challenges

Cited by 12 publications

References 17 publications

Research Trends and Challenges of Using CRISPR/Cas9 for Improving Rice Productivity

Research Trends and Challenges of Using CRISPR/Cas9 for Improving Rice Productivity

Genetic and Molecular Mechanisms Conferring Heat Stress Tolerance in Tomato Plants

Efficient CRISPR/Cas-Mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties

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