Diclofenac-induced cytotoxicity in cultured carp leukocytes

Němcová, Monika; Pikula, Jiří; Zukal, Jan; Seidlová, Veronika

doi:10.33549/physiolres.934609

Cited by 4 publications

(1 citation statement)

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“…Imidacloprid and PM are generally considered as low toxicity substances to warmblooded mammals, but very little is known about the impact of toxic substances on heterothermic bats during hibernation. The aim of the study was to assess the toxic effect of two commonly used insecticides: PM and IMI, on bat-derived cells in culture, because cell cultures represent a unique alternative tool for studying toxin (Kovacova et al 2016;Abdelsalam et al 2020;Nemcova et al 2020) and pathogen effects (He et al 2014;Flieger et al 2016) in highly protected species. As the liver represents a biotransformation organ in the metabolism of xenobiotics, liver cells are the gold standard for toxicological testing of a wide range of chemicals (Soldatow et al 2013).…”

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confidence: 99%

Torpor/hibernation cycle may enhance the risk of insecticides for bats: an in vitro study

et al. 2022

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Exposure to pollutants is considered one of the potential reasons of population declines in bats. In the context of previous studies, we managed to create and keep a wide collection of cell lines from European bat species. Liver cells were chosen for testing, as they represent the preferred model for toxicological studies. Bats are protected, cell lines replacing experimental animals thus represent a unique opportunity to examine effects of pollutants which animals are exposed to in their environments. Moreover, cell incubation temperature variation may simulate physiological states of heterothermic bats. Liver cell lines were cultivated to the required cell number. Exposure to five different concentrations of permethrin (PM) and imidacloprid (IMI) were used to determine cytotoxic effects of these pesticides on Nyctalus noctula-derived liver cells cultivated at 37 °C and 8 °C for 24 h. An assay based on the measurement of activity of lactate dehydrogenase released from damaged cells was used for quantitating cytotoxicity. Cytotoxicity of IMI ranged from 0% to 47% and from 56% to 67% at 37 °C and 8 °C, respectively. Cytotoxicity of PM ranged from 36% to 56% and from 43% to 88% at 37 °C and 8 °C, respectively. Permethrin was tested on the cells at an order of magnitude lower concentrations; even so, higher degree of cytotoxicity was recorded. Imidacloprid was more toxic to bat liver cells at a hibernation temperature than at body temperature of 37 °C.

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confidence: 99%