1,25-Dihydroxyvitamin D₃Attenuates of Expression of Experimental Murine Lupus of MRL/1 Mice

Abstract

The murine strain MRL/l spontaneously develops a systemic lupus erythematosus (SLE)-like syndrome. An increased number of T cells and polyclonal T helper cell activity has been described in these mice suggesting a potential role for 1,25-dihydroxyvitamin-D3 [1,25-D3], an antiproliferative hormone selecting the T-helper lymphocyte subset. One month old MRL/l mice were submitted or not to 1,25-D3 0.1 microgram for 4 weeks, then 0.15 microgram given i.p. every other day for 18 weeks while maintained on a low calcium chow. Dermatologic lesions, i.e. alopecia, necrosis of the ear and scab formation, were completely inhibited by 1,25-D3 therapy. By 20 weeks, all mice had developed proteinuria. However, the degree of proteinuria was somewhat reduced in treated mice as assessed by urine protein/creatine ratios (less than 4 vs greater than 4 in treated vs untreated mice respectively). Moreover, a trend for a reduction in serum titers for anti-ssDNA antibodies was observed at 18 weeks. The active vitamin D metabolite had no effect on the development of the generalized lymphoid hyperplasia. Hypercalcemia developed when 1,25-D3 was increased to 0.15 microgram (2.62 +/- 0.12 vs 1.97 +/- 0.07 mmol/l, treated vs untreated mice respectively). These results suggest a beneficial role of 1,25-D3 in the prevention or attenuation of some manifestations of murine SLE, a model sharing many immunologic features with human SLE.

Vitamin D and type 2 diabetes mellitus: Where do we stand?

“…In addition, more and more observational, experimental and also interventional studies have recently emerged to demonstrate the contribution of significant VTD supplementation to different health benefits. Of these so far, it is worth noting its positive effects on cancer [3], sarcopenia in the elderly [4], multiple sclerosis [5], hypertension [6], cardiovascular disease [7], congestive heart failure [8], lupus erythematosus [9], arthritis [10], type 1 diabetes [11] and various infectious diseases [12].…”

The anti‐inflammatory effects of 1,25‐dihydroxyvitamin D3 on Th2 cells in vivo are due in part to the control of integrin‐mediated T lymphocyte homing

“…The effect of 1,25(OH) 2 D 3 has been studied in Th1 models and was shown to prevent Th1-mediated autoimmune disease in animal models for experimental allergic encephalomyelitis, systemic lupus erythematosus, rheumatoid arthritis and inflammatory bowel disease [18][19][20][21]. These studies indicate that one of the functions of 1,25(OH) 2 D 3 in the immune system is to inhibit Th1 differentiation.…”

La vitamine D : effets « classiques », « non classiques » et évaluation du statut du patient

“…), il apparaît que l'explication la plus probable de cet effet antitumoral est lié au fait que dans certains tissus, la 1,25(OH)2D régule un certain nombre de gènes qui contrôlent la prolifération cellulaire, et stimule d'autres gènes qui, eux, inhibent l'angiogénèse et induisent l'apoptose des cellules tumorales [34][35][36][37][38]. Les études montrent que la 1,25(OH)2D réduit la prolifération lymphocytaire et la production de certaines cytokines [39] et peut atténuer ou même prévenir différentes maladies dans des modèles animaux, principalement murins d'encéphalomyélite (modèle de la sclérose en plaques) [40], de lupus [41], de polyarthrite rhumatoïde [42], de diabète de type-1 [43]. .…”