Preparation of Poly(D,L-Lactide/Glycolide) Nanoparticles of Controlled Particle Size Distribution: Application of Experimental Designs

Abstract

This study investigates the use of the solvent evaporation method for preparing acid and glycolic acid-based copolymer nanoparticles. Initially, appropriate technological and formulation factors for elaboration of polymeric particles were selected by screening.Most favourable results were obtained using polyvinyl alcohol as a dispersing agent and a high pressure homogenizer to reduce the droplet size of the emulsion initially formed. On the basis of the conclusion thus drawn, a composite rotational experimental design was employed to evaluate the joint influence of three formulation variables (phase volume ratio of the emulsion formed, polymer concentration and homogenization pressure) on the micromeritic properties of the suspension finally obtained (mean particle size, coefficient of variation and polydispersity of the particle size distribution). Analysis of variance corresponding to the experimental design, showed a significant influence of the volume phase ratio and the polymer concentration on the mean particle size and the coefficient of variation, whereas the polydispersity is also affected by the homogenization pressure.Considering this information, a 3* experimental factorial design was then selected to investigate the possible interaction between the phase volume ratio and the concentration of polyvinyl alcohol in the aqueous phase. Analysis of variance and subsequent sequencial regression analysis evidenced last hypothesis providing the way to determine the experimental conditions required to achieve a specific particle size distribution.

Susceptibility of cobalt ferrite nanoparticles dispersed in polylactic acid microspheres

“…This study reports on dynamical susceptibility (DS) investigation of a composite system consisting of cobalt ferrite nanoparticles dispersed in micrometer-sized PLA spheres. The field ðHÞ dependence of the peak frequency ðf p Þ of the imaginary component ðw 00 Þ of the DS curve is described using the picture of a superparamagnetic particle in an asymmetrical double-well potential [3].Preparation of the PLA-based composite containing cobalt ferrite (CoFe 2 O 4 ) nanoparticles was carried out using the solvent evaporation technique [4]. The magnetic nanoparticles were incorporated in the polymeric template by mixing d,l-lactide with oleic acidcoated cobalt ferrite-based magnetic fluid.…”

“…Preparation of the PLA-based composite containing cobalt ferrite (CoFe 2 O 4 ) nanoparticles was carried out using the solvent evaporation technique [4]. The magnetic nanoparticles were incorporated in the polymeric template by mixing d,l-lactide with oleic acidcoated cobalt ferrite-based magnetic fluid.…”

Development of Acid-Resistant Alginate/Trimethyl Chitosan Nanoparticles Containing Cationic β-Cyclodextrin Polymers for Insulin Oral Delivery

“…Optimization, which is usually carried out by response surface methods (RSMs), is used to achieve optimized terms for preparing a drug delivery system with desirable physicochemical features. Although different methods exist to conduct these studies, in this study, the Placket Burman experimental design method was used for screening (26,45,46).…”

Micelle-templated, poly(lactic-<em>co</em>-glycolic acid) nanoparticles for hydrophobic drug delivery

“…10 The most common methods employed to synthesize PLGA NPs are nanoprecipitation 11 and emulsion-based processes. 12 Using these approaches, particles 200 nm in size can be easily and uniformly produced by optimizing PLGA concentration, 13,14 PLGA-solvent interactions, 15 continuous-and dispersedphase viscosities, 16 or the rate of mixing and shear stress 17 applied for emulsion formation. However, synthesis of sub-100 nm PLGA NPs, as might be required for systemic delivery, has proven particularly challenging.…”