Um Spin Coater Artesanal Baseado Em Lixo Eletrônico: Uma Alternativa Versátil E De Baixo Custo

Abstract

A HOMEMADE SPIN COATER BASED ON ELECTRONIC WASTE: A VERSATILE AND LOW COST ALTERNATIVE. High-precision equipment generally represents a significant portion of a laboratory budget, leading the access by laboratories with limited financial resources difficult. Then, adaptation of materials such as electronic trash may be a key factor to overcome financial obstacles. As the technological advancement brings with it an increase in the disposal of pieces of equipment considered obsolete, these pieces however can still be used in the construction of new equipment. The present work shows the employment of a scrapped computer HD in addition to an Arduino microcontroller and other electronic components to build a low-cost spin coater. The thickness of films obtained in the handmade equipment presented approximately five percent of difference in relation to films obtained in a commercial equipment.

Oral Squamous Cell Carcinoma Lipid Evaluation in Gingiva Tissue Stored in TRIzol via Shotgun Lipidomics and MALDI Mass Spectrometry Imaging

“…A DHB solution 30 mg mL –1 in methanol:water (70:30) with 1% of trifluoroacetic acid or a DAN solution 3.3 mg mL –1 in methanol:water (90:10) was sprayed over the tissue slice at a flow rate of 300 μL min –1 and nebulizer gas at 2 bar using an in-house built sprayer. During the matrix covering, the glass slide was kept under rotation at 300 rpm, using an in-house spin coater built as described by Blaskievicz and co-workers . The matrix application system is shown in Figure S1 (Supporting Information).…”

“…During the matrix covering, the glass slide was kept under rotation at 300 rpm, using an in-house spin coater built as described by Blaskievicz and co-workers. 19 The matrix application system is shown in Figure S1 (Supporting Information).…”