RhoA/ROCK-1 Signaling Pathway and Oxidative Stress in Coronary Artery Disease Patients

Abstract

Introduction Coronary artery disease (CAD) is an ischemic condition that occurs as a result of partial or complete interruption of blood flow by narrowing or complete blockage of the vessels supplying the heart, which are called coronary arteries. Our objective in this study is to investigate the RhoA/Rho-associated kinase (ROCK)-1 signaling pathway and oxidative stress in CAD patients. Methods A total of 81 individuals aged between 40-70 years - including 45 patients (15 females and 30 males) who were admitted to the Artvin State Hospital Cardiovascular Surgery Clinic and were diagnosed with CAD and 36 healthy volunteers (15 females and 21 males) - participated in this study. Serum samples were tested for total cholesterol, triglyceride, low-density lipoprotein, high-density lipoprotein, malondialdehyde (MDA), superoxide dismutase (SOD), RhoA, and ROCK-1 values. Results Serum RhoA, MDA levels, and ROCK-1 activity in the CAD group were found to be statistically significantly higher than in the control group ( P <0.001). Concordantly, serum SOD activity was found to be statistically significantly lower in the CAD group than in the control group ( P <0.001). Conclusion Inhibition of the activity of RhoA/ROCK-1 pathway would be beneficial in treating cardiovascular diseases since this pathway plays an important role in the development of these diseases.

Hesperidin promotes gastric motility in rats with functional dyspepsia by regulating Drp1-mediated ICC mitophagy

“…Malondialdehyde (MDA) is the carbonyl group during lipid peroxidation and is widely used to determine oxidative stress. SOD activity decreases during oxidative stress and produces excess lipid peroxidation product MDA, which can indirectly reflect the degree of mitochondrial peroxidative damage (Tian et al, 2019;Dokumacioglu et al, 2021). The results of this study showed that compared with the control group, the serum MDA content of the model group was increased, the SOD content was decreased, and the mitochondrial ROS level was increased.…”

“…The reduction in the activity of antioxidant enzymes such as superoxide dismutase (SOD) promotes oxidative attack on cells. Once the redox balance is disrupted, SOD reacts with oxygen molecules and scavenges superoxide anions that are harmful to the organism thus achieving antioxidant stress (Liu et al, 2020;Dokumacioglu et al, 2021). Malondialdehyde (MDA) is the carbonyl group during lipid peroxidation and is widely used to determine oxidative stress.…”

Experimental research on the evaluation of left ventricular systolic function by layered speckle tracking before and after berberine treatment in a cardiac hypertrophy rat model

“…The RhoA/ROCK pathway is involved in various cardiovascular diseases (25), such as heart failure, and may also be very involved in ISO-induced cardiac hypertrophy (26). Tian et al confirmed that the ROCK inhibitor BBR has cardioprotective effects in a model of myocardial infarction, and is considered a novel adjuvant therapy to reduce myocardial injury (27).…”

Identification of novel prognostic targets in coronary artery disease and related complications using bioinformatics and next generation sequencing data analysis

“…In recent years, scholars worldwide have made remarkable achievements in biomarkers and aberrant molecular signaling pathways are involved in the CAD. Data identified several signaling pathways include NF-κB signaling pathway [19], RhoA/ROCK-1 signaling pathway [20], Wnt signaling pathway [21], TLR4 signaling pathway [22] and transforming growth factor β signaling pathway [23], and genes include SCARB1 [24], TP53 [25], RRM1, RRM2 and ERCC2 [26], IL17RA [27] and COX2 [28] that were significantly associated with CAD. In particular, the novel molecular characteristics can be tested in initial risk assessment, the recognition of better specific biomarkers, and the advancement of clinic treatment and survival.…”