Lidocaine and Pinacidil Added to Blood versus Crystalloid Cardioplegic Solutions: Study in Isolated Hearts

Abstract

ObjectiveThe present study aimed the functional recovery evaluation after long term of cardiac arrest induced by Custodiol (crystalloid-based) versus del Nido (blood-based) solutions, both added lidocaine and pinacidil as cardioplegic agents. Experiments were performed in isolated rat heart perfusion models.MethodsMale rat heart perfusions, according to Langendorff technique, were induced to cause 3 hours of cardiac arrest with a single dose. The hearts were assigned to one of the following three groups: (I) control; (II) Custodiol-LP; and (III) del Nido-LP. They were evaluated after ischemia throughout 90 minutes of reperfusion. Left ventricular contractility function was reported as percentage of recovery, expressed by developed pressure, maximum dP/dt, minimum dP/dt, and rate pressure product variables. In addition, coronary resistance and myocardial injury marker by alpha-fodrin degradation were also evaluated.ResultsAt 90 minutes of reperfusion, both solutions had superior left ventricular contractile recovery function than the control group. Del Nido-LP was superior to Custodiol-LP in maximum dP/dt (46%±8 vs. 67%±7, P<0.05) and minimum dP/dt (31%±4 vs. 51%±9, P<0.05) variables. Coronary resistance was lower in del Nido-LP group than in Custodiol-LP (395%±50 vs. 307%±13, P<0.05), as well as alpha-fodrin degradation, with lower levels in del Nido-LP group (P<0.05).ConclusionDel Nido-LP cardioplegia showed higher functional recovery after 3 hours of ischemia. The analysis of alpha-fodrin degradation showed del Nido-LP solution provided greater protection against myocardial ischemia and reperfusion (IR) in this experimental model.

Effects of a collagen hyaluronic acid silk‐fibroin patch with the electroconductive element polyaniline on left ventricular remodeling in an infarct heart model

“…Hemodynamic data, obtained from Langendorff isolated heart model, and BNP levels did not present differences. The isolated heart model is a common method to analyze hemodynamic parameters from the heart, minimizing the interference of external issues 17 . BNP is a natriuretic peptide secreted by ventricular cardiomyocytes in response to myocardial stretching induced by hypervolemia 28 .…”

“…Immediately after cardiectomy, three hearts from each group were harvested for isolated heart perfusion. Those hearts were fixed via the ascending aorta in the perfusion system (Radnoti LLC) using modified Krebs Henseleit (KH) solution (mM: 118, NaCl; 25, NaHCO 3 ; 11, D‐glucose; 4.7, KCl; 1.22 MgSO 4 ·7H 2 O; 1.21, KH 2 PO 4 ; and 1.8 CaCl 2 ·2H 2 O) with retrograde flow under the constant pressure of 70 mmHg, according to the classic Langendorff technique 17 …”

“…All data were acquired using PowerLab® (ADInstruments, Colorado Springs, Colorado) system and analyses were conducted using Lab Chart Pro® software (ADInstruments, Colorado Springs, Colorado). Harvested hearts were compared between a patch group comprising both patches and MI control group 17 …”

“…The isolated heart model is a common method to analyze hemodynamic parameters from the heart, minimizing the interference of external issues. 17 BNP is a natriuretic peptide secreted by ventricular cardiomyocytes in response to myocardial stretching induced by hypervolemia. 28 Natriuretic peptides inhibit the renin-angiotensin-aldosterone system, stimulate peripheral vasodilation, and increase urinary excretion of sodium and water.…”

Comment on “Lidocaine and pinacidil added to blood versus crystalloid cardioplegic solutions: study in isolated hearts”

“…In the issue 33(3) of the Brazilian Journal of Cardiovascular Surgery, Carmo et al. [1] published a paper entitled "Lidocaine and pinacidil added to blood versus crystalloid cardioplegic solutions: study in isolated hearts". The authors report on studies in rat hearts using a Langendorff model.…”