Inhibition of Na+, K+ -ATPase with ouabain is detrimental to equine blastocysts

Júnior, Agnelo Douglas do Nascimento; Marques, Juliana Carla Cavalcanti; Cezar, Allan Rodolf Ribeiro; Batista, André Mariano; Kastelic, John P.; Câmara, Diogo Ribeiro

doi:10.21451/1984-3143-ar2019-0079

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…These ion channels such as NA + , K + , ATPase are essential for the accumulation of blastocoel and subsequently yolk sac fluid in equine embryos [ 92 ]. Their inhibition is detrimental for embryo development [ 93 ]. Thus, both reduced ATP production and disturbed ion channels’ function may impact embryo growth, although effects of mares’ age on embryo growth are controversial [ 24 – 28 ].…”

Section: Discussionmentioning

confidence: 99%

Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass

Derisoud

Jouneau

Dubois³

et al. 2022

BMC Genomics

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Background Breeding a mare until she is not fertile or even until her death is common in equine industry but the fertility decreases as the mare age increases. Embryo loss due to reduced embryo quality is partly accountable for this observation. Here, the effect of mare’s age on blastocysts’ gene expression was explored. Day 8 post-ovulation embryos were collected from multiparous young (YM, 6-year-old, N = 5) and older (OM, > 10-year-old, N = 6) non-nursing Saddlebred mares, inseminated with the semen of one stallion. Pure or inner cell mass (ICM) enriched trophoblast, obtained by embryo bisection, were RNA sequenced. Deconvolution algorithm was used to discriminate gene expression in the ICM from that in the trophoblast. Differential expression was analyzed with embryo sex and diameter as cofactors. Functional annotation and classification of differentially expressed genes and gene set enrichment analysis were also performed. Results Maternal aging did not affect embryo recovery rate, embryo diameter nor total RNA quantity. In both compartments, the expression of genes involved in mitochondria and protein metabolism were disturbed by maternal age, although more genes were affected in the ICM. Mitosis, signaling and adhesion pathways and embryo development were decreased in the ICM of embryos from old mares. In trophoblast, ion movement pathways were affected. Conclusions This is the first study showing that maternal age affects gene expression in the equine blastocyst, demonstrating significant effects as early as 10 years of age. These perturbations may affect further embryo development and contribute to decreased fertility due to aging.

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Section: Discussionmentioning

confidence: 99%

Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass

Derisoud

Jouneau

Dubois³

et al. 2022

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…These ion channels such as NA + , K + , ATPase are essential for the accumulation of blastocoel and subsequently yolk sac fluid in equine embryos 86 . Their inhibition is detrimental for embryo development 87 . Thus, both reduced ATP production and disturbed ion channels’ function may impact embryo growth, although effects of mares’ age on embryo growth are controversial 24–28 .…”

Section: Discussionmentioning

confidence: 99%

“…Their inhibition is detrimental for embryo development 87 . Thus, both reduced ATP production and disturbed ion channels' function may impact embryo growth, although effects of mares' age on embryo growth are controversial [24][25][26][27][28] .…”

Section: Discussionmentioning

confidence: 99%

Maternal age affects equine Day 8 embryo gene expression both in trophoblast and inner cell mass

Derisoud

Jouneau

Dubois³

et al. 2021

Preprint

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Background: Increased embryo loss as mares become older is a major consideration for breeders as older animals are currently used for reproduction in the equine industry. Lower embryo quality has been pointed out as partly responsible for this reduced fertility. Here, the effect of mare's age on blastocysts' gene expression was explored. Day 8 post ovulation embryos were collected by uterine flushing from multiparous young (YM, 6-year-old, N = 5) and older (OM, > 10-year-old, N = 6) non-nursing Saddlebred mares, inseminated with the semen of one stallion. Pure (TE_part) or inner cell mass enriched (ICMandTE) trophoblast were obtained by embryo bisection and paired end, non-oriented RNA sequencing (Illumina, NextSeq500) was performed on each hemi-embryo. To discriminate gene expression in the ICM from that in the TE, deconvolution (DeMixT R package) was used on the ICMandTE dataset. Differential expression was analyzed (DESeq2) with embryo sex and diameter as cofactors using a false discovery rate < 0.05 cutoff. Functional annotation and classification of differentially expressed genes and gene set enrichment analysis were also performed. Results: Maternal aging did not affect embryo recovery rate, embryo diameter nor total RNA quantity but modified gene expression in equine D8 embryos. The expression of genes in the ICM seemed to be more altered by maternal aging than in the TE. In both compartments, the expression of genes involved in mitochondrial function, translation and transcription due to chromatin modification were disturbed by maternal age. Mitosis, signaling and adhesion pathways and embryo development were particularly decreased in the ICM hemi-embryos from old mares. Finally, in TE, ion movement related genes were affected. Conclusions: This is the first study showing an effect of maternal age on gene expression in the equine blastocyst at Day 8 post ovulation. Maternal age, even for mares as young as 10 years old, disturbs mitosis, translation and transcription, cell signaling and adhesion as well as mitochondrial function and cell commitment of horse embryos. These perturbations may affect further embryo development and contribute to decreased fertility due to aging.

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Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak

Pei

Song²,

Bao

et al. 2022

BMC Vet Res

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Background Ovarian follicle fluid (FF) as a microenvironment surrounding oocyte plays critical roles in physio-biochemical processes of follicle development and oocyte maturation. It is hypothesized that proteins in yak FF participate in the physio-biochemical pathways. The primary aims of this study were to find differentially expressed proteins (DEPs) between mature and immature FF, and to elucidating functions of the mature and immature FF in yak. Results The mature and immature FF samples were obtained from three healthy yaks that were nonpregnant, aged from four to five years, and free from any anatomical reproductive disorders. The FF samples were subjected to mass spectrometry with the isobaric tags for relative and absolute quantification (iTRAQ). The FF samples went through correlation analysis, principle component analysis, and expression pattern analysis based on quantification of the identified proteins. Four hundred sixty-three DEPs between mature and immature FF were identified. The DEPs between the mature and immature FF samples underwent gene ontology (GO), Kyoto encyclopedia of genes and genomes (KEGG), and protein-protein interaction (PPI) analysis. The DEPs highly expressed in the mature FF mainly took parts in the complement and coagulation cascades, defense response, acute-phase response, response to other organism pathways to avoid invasion of exogenous microorganisms. The complement activation pathway contains eight DEPs, namely C2, C5, C6, C7, C9, C4BPA, CFH, and MBL2. The three DEPs, CATHL4, CHGA, and PGLYRP1, take parts in defense response pathway to prevent invasion of exogenetic microorganism. The coagulation cascades pathway involves many coagulation factors, such as F7, F13A1, FGA, FGB, FGG, KLKB1, KNG1, MASP1, SERPINA1, and SERPIND1. While the DEPs highly expressed in the immature FF participated in protein translation, peptide biosynthetic process, DNA conformation change, and DNA geometric change pathways to facilitate follicle development. The translation pathway contains many ribosomal proteins, such as RPL3, RPL5, RPS3, RPS6, and other translation factors, such as EIF3J, EIF4G2, ETF1, MOV10, and NARS. The DNA conformation change and DNA geometric change involve nine DEPs, DDX1, G3BP1, HMGB1, HMGB2, HMGB3, MCM3, MCM5, MCM6, and RUVBL2. Furthermore, the expressed levels of the main DEPs, C2 and SERPIND1, were confirmed by western blot. Conclusions The differential proteomics revealed the up-regulated DEPs in mature FF take parts in immunoreaction to prevent invasion of microorganisms and the up-regulated DEPs in immature FF participate in protein synthesis, which may improve our knowledge of the follicular microenvironment and its biological roles for reproductive processes in yak. The DEPs, C2 and SERPIND1, can be considered as protein markers for mature yak follicle.

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Inhibition of Na+, K+ -ATPase with ouabain is detrimental to equine blastocysts

Cited by 3 publications

References 22 publications

Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass

Maternal age affects equine day 8 embryo gene expression both in trophoblast and inner cell mass

Maternal age affects equine Day 8 embryo gene expression both in trophoblast and inner cell mass

Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak

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