Chlorogenic acid improves the quality of boar semen processed in Percoll

Rabelo, S. S.; Resende, Carla Oliveira; Pontelo, Thaís Preisser; Chaves, Bruna Resende; Pereira, Bárbara Azevedo; Silva, William Eduardo da; Peixoto, Juliano Vogas; Zangerônimo, Márcio Gilberto

doi:10.21451/1984-3143-ar2019-0021

Cited by 3 publications

(3 citation statements)

References 33 publications

(39 reference statements)

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“…Many natural compounds with scavenging activity against LPO induced in vitro in human spermatozoa were studied [ 18 , 19 , 41 , 42 ]. Recently, Rabelo et al [ 32 ] observed that the addition of CGA to the semen extender improved the quality of boar sperm processed by Percoll technique or unprocessed, better than that obtained with vitamin E supplementation. The benefits expected from the use of CGA in human semen were observed in this research.…”

Section: Discussionmentioning

confidence: 99%

“…It displays different pharmacological activities such as antioxidant, anti-inflammatory, anti-microbial, hepatoprotective, anticancer, anti-lipidemic, and anti-diabetic [ 29 ]. A concentration of 100 µM CGA yielded beneficial effects on sperm motility, viability, and plasma membrane integrity in boar semen during freezing [ 30 ] and cooling at 15 °C [ 31 , 32 ]. CGA also displayed a positive effect in in vitro production of porcine embryos and a protection against DNA damage induced by OS in oocytes [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

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Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen—Thawed Protocol

et al. 2021

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The study evaluated the chlorogenic acid (CGA) antioxidant potential on oxidative stress (OS) induced in vitro in human spermatozoa and during cryopreservation procedure. Swim-up selected spermatozoa were treated with 100 µM CGA, 100 µM H2O2 to induce lipid peroxidation (LPO), and with both compounds and the effects on mitochondrial membrane potential (MMP) by JC-1, DNA integrity by acridine orange (AO), and sperm ultrastructure by transmission electron microscopy (TEM), were evaluated. CGA antioxidant activity was assessed by measuring malondialdehyde (MDA) and F2-isoprostanes (F2-IsoPs) in the media. The CGA protective activity and the immunolocalization of Phospho-AMPKα (Thr172) were explored in frozen-thawed sperm. CGA was not toxic for sperm motility, DNA integrity and MMP. The increase in MDA (p < 0.05) and F2-IsoPs (p < 0.001), DNA damage (p < 0.01) and low MMP (p < 0.01) levels after H2O2 treatment were reduced in presence of CGA as well as the percentage of broken plasma membranes (p < 0.01) and altered acrosomes (p < 0.01) detected by TEM. Treated frozen-thawed spermatozoa showed increased sperm motility (p < 0.01), DNA integrity (p < 0.01), MMP (p < 0.01), reduced MDA (p < 0.01) and increased sperm percentage with Phospho-AMPKα labelling in the head (p < 0.001). CGA can be used to supplement culture media during semen handling and cryopreservation where OS is exacerbated.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen—Thawed Protocol

et al. 2021

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“…It has been reported that the sperm count of epididymis increased by 20% after 5 weeks of CGA administration in rats [ 24 ]. CGA improved the quality of cooled and frozen-thawed boar sperm [ 25 , 26 , 27 ]. Furthermore, CGA improved the antioxidant capacity of human sperm in vitro and during the frozen-thaw stage [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Chlorogenic Acid Improves Quality of Chilled Ram Sperm by Mitigating Oxidative Stress

Wang

Zhang

Sohail

et al. 2022

Animals

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The purpose of this study was to investigate whether the addition of chlorogenic acid (CGA) to a sheep semen extender could improve the quality of chilled sheep sperm. Ejaculates (n = 80) were collected from five Hu rams with an artificial vagina. The ejaculates were mixed and divided into five equal parts, diluted with a CGA-free Tris–egg yolk extender (control), or supplemented with 0.2, 0.4, 0.8, and 1.2 mg/mL. The sperm kinematic parameters (viability, progressive motility), functional integrity of plasma membrane and acrosome, adenosine triphosphate (ATP) concentration and antioxidant parameters (Catalase (CAT), Superoxide dismutase (SOD) activity, total antioxidant capacity (T-AOC), ROS level and Malondialdehyde (MDA) content) were evaluated during storage of the semen. The results indicated that: PM, plasmatic membrane integrity and acrosomal integrity in 0.8 mg/mL CGA were higher (p < 0.05) from day 1 to 5. The ROS level in CGA groups was lower than the control (p < 0.05). CAT, SOD, ATP, and T-AOC were highest at 0.8 mg/mL concentration within 1 to 5 days. The above results indicated that the right concentration of CGA improved the quality of Hu ram sperm during chilling storage.

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Integrating sperm cell transcriptome and seminal plasma metabolome to analyze the molecular regulatory mechanism of sperm motility in Holstein stud bulls

Zhang

et al. 2023

Journal of Animal Science

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Considering that artificial insemination is the most widely used assisted reproductive technique in the dairy industry, the semen quality of bulls is very important for selecting excellent stud bulls. Sperm motility is one of the important traits of semen quality, and related genes may be regulated by environmental factors. Seminal plasma can affect sperm cell transcriptome and further affect sperm motility through exosome or other processes. However, the molecular regulation mechanism of bull sperm motility has not been studied by combining the sperm cell transcriptome with seminal plasma metabolome. The number of motile sperm per ejaculate (NMSPE) is an integrated indicator for assessing sperm motility in stud bulls. In the present study, we selected 7 bulls with higher NMSPE (5,698.55 million +/- 945.40 million) as group H and 7 bulls with lower NMSPE (2,279.76 million +/- 1,305.69 million) as group L from 53 Holstein stud bulls. The differentially expressed genes (DEG) in sperm cells were evaluated between the two groups (H vs. L). We conducted gene co-expression network analysis (WGCNA) on H and L groups of bulls, as well as two monozygotic (MZ) twin Holstein bulls with different NMSPE values, to screen candidate genes for NMSPE. The regulatory effect of seminal plasma metabolome on the candidate genes of NMSPE was also investigated. A total of 1,099 differentially expressed genes (DEG) were identified in the sperm cells of H and L groups. These DEGs were primarily concentrated in energy metabolism and sperm cell transcription. The significantly enriched KEGG pathways of the 57 differential metabolites were the aminoacyl-tRNA biosynthesis pathway and vitamin B6 metabolism pathway. Our study discovered 14 genes as the potential candidate markers for sperm motility, including FBXO39. We observed a broad correlation between transcriptome of sperm cells and seminal plasma metabolome, such as three metabolites, namely, mesaconic acid, 2-coumaric acid, and 4-formylaminoantipyrine, might regulate FBXO39 expression through potential pathways. The genes related to seminal plasma metabolites expressed in sperm cells are not only located near the quantitative trait loci of reproductive traits, but also enriched in the GWAS signal of sire conception rate. Collectively, this study was the first to investigate the interplays among transcriptome of sperm cells and seminal plasma metabolome from Holstein stud bulls with different sperm motility.

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Chlorogenic acid improves the quality of boar semen processed in Percoll

Cited by 3 publications

References 33 publications

Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen—Thawed Protocol

Protective Effect of Chlorogenic Acid on Human Sperm: In Vitro Studies and Frozen—Thawed Protocol

Chlorogenic Acid Improves Quality of Chilled Ram Sperm by Mitigating Oxidative Stress

Integrating sperm cell transcriptome and seminal plasma metabolome to analyze the molecular regulatory mechanism of sperm motility in Holstein stud bulls

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