Evaluation of the proteomic profiles of ejaculated spermatozoa from Saanen bucks (Capra hircus)

Abstract

The Saanen goat breed has been widely explored in breeding programmes; however, there are few reports about the breed's genetic and molecular composition. Thus, this study aimed to characterize the proteomic profile of spermatozoa from Saanen breeding goats. Five breeding animals with proven fertility were selected, the spermatozoa were collected, and the protein was extracted. Subsequently, the proteins were separated and analysed by two-dimensional electrophoresis and mass spectrometry; the proteins were then identified with the SwissProt database. A total of 31 proteins involved in reproduction were identified, including binding proteins on spermatozoa for fusion with the egg, acrosomal membrane proteins, metabolic enzymes, heat shock proteins, cytoskeletal proteins and spermatozoa motility proteins. The characterization of such proteins clarifies the molecular mechanisms of spermatogenesis and the modifications that ensure the success of fertilization.

Shotgun Proteomics-based distinctive fingerprint of buffalo sperm surface unravels over-representation of proteins driving spermatogenesis, motility, and fertility

“…A wide range of diversity was observed in the more than 1600 proteins and isoforms predicted to be present on the buffalo spermatozoa vis-à-vis their molecular weight, pI, distribution of expression across chromosomes and functional roles (Fig. 3), as reported in other species [48][49][50][51][52][53][54]. These protein inventory lists, such as those generated in this work will shed light on peripheral and transmembrane proteins hitherto unknown to exist on the sperm surface.…”

Shotgun Proteomics-based distinctive fingerprint of sperm-surface revealed over- representation of proteins driving spermatogenesis, motility, and fertility

“…A wide range of diversity was observed in the more than 1600 proteins and isoforms predicted to be present on the buffalo spermatozoa vis-à-vis their molecular weight, pI, distribution of expression across chromosomes and functional roles (Fig. 3), as reported in other species [48][49][50][51][52][53][54]. Several proteins were found to be unique to each of the treatment groups demonstrating indicating their disparate effects on disrupting the non-covalent (2X-DPBS) or covalent interactions (PI-PLC) among the proteins of sperm surface.…”

Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus)

“…Proteomic analysis has been used to describe the sperm and seminal plasma proteome either in non-mammal species, such as fish (Ciereszko et al, 2017;Dietrich et al, 2017), and in mammals, such as murine (Baker et al, 2008;Vicens et al, 2017), porcine (Perez-Patiño et al, 2016;Recuero et al, 2019;Luongo et al, 2020), equine (Swegen et al, 2015;Guasti et al, 2020), ovine (Cardozo et al, 2006;Pini et al, 2016), caprine (Pinto et al, 2019;Zhu et al, 2020;Martínez-Fresneda et al, 2021), bovine (Peddinti et al, 2008;Byrne et al, 2012), non-human primates (Skerget et al, 2013), and human (Martínez-Heredia et al, 2006), but not yet in any cetacean species. Furthermore, such studies have permitted the identification of proteins that can act as biomarkers of fertility (Dacheux et al, 2012;Li et al, 2016;Rahman et al, 2017;Druart and de Graaf, 2018;Pérez-Patiño et al, 2018;Druart et al, 2019), their relevance for sperm preservation (Soleilhavoup et al, 2014;Parrilla et al, 2019;Peris-Frau et al, 2019;Ryu et al, 2019;Bajuk et al, 2020;De Lazari et al, 2020) or involvement in sperm functional traits (Intasqui et al, 2016;Bezerra et al, 2019;De Lazari et al, 2019).…”