The effects of methanol extract of Galium verum L on cardiac redox state in hypertensive rats

Abstract

The aim of this study was to assess the effects of methanol extract of G. verum on redox status of isolated heart of spontaneously hypertensive rats after ischemia. Twenty-four Wistar albino rats were divided into three groups: untreated control rats and rats that received 125 and 250 mg/kg G. verum extract for 4 weeks per os. Index of lipid peroxidation (measured as TBARS) and parameters of antioxidative defence system such as level of reduced glutathione (GSH) and activities of catalase (CAT) and superoxide dismutase (SOD) were spectrophotometrically determined in heart homogenate. The index of lipid peroxidation in heart tissue was lower in both treated groups compared to the control group. On the other hand, the activity of SOD was significantly higher after consumption of both doses, while the activity of CAT was significantly higher only after treatment with a higher dose of extract. Based on our results we might conclude that 4-week treatment with methanol extracts of G. verum has the potential to modulate myocardial redox signaling after ischemia, thus significantly alleviating cardiac oxidative stress and exerting dose-dependent antioxidant properties. Future studies are certainly necessary to fully clarify the role of this plant species in myocardial I-R injury.

An Investigation on the Antioxidant Capabilities, Chemical Analysis and Potential Green Applications of the Yellow Bedstraw (Galium Verum) Ethanol Extract

“…The most used extraction mediums are hydromethanolic extract (Xie and Schaich, 2014;Lakić et al, 2010), hydroethanolic extract (Bradic, et al, (Mocan et al, 2019;Wairata et al, 2022). These extracts have been obtained using different methods: maceration (Lakić et al, 2010;Bota et al, 2021;Turcov et al, 2022), re ux extraction (Turcov et al, 2022;Milevic et al, 2022;Bradic et al, 2019) ultrasound extraction (Bota et al, 2021;Turcov et al, 2022) decoction (Schmidt et al, 2014) or microwave extraction (Mocan et al, 2019).…”