Analysis of the applicative potential of pigments extracted from bacterial isolates of mangrove soil as topical UV protectants

Patki, Jyoti; Singh, Suvidha; Singh, Sunita; Padmadas, Naveen; Dasgupta, Debjani

doi:10.1590/s2175-97902020000419127

Cited by 6 publications

(4 citation statements)

References 32 publications

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“…Besides, the concentration of 10% (w/v) of carotenoids by Brevibacterium sp. has SPF value of 5.311 (Patki et al, 2021). Therefore, it is observed that SPF value increases as the pigment concentration increases.…”

Section: Potential As Sunscreen Agentmentioning

confidence: 92%

“…There are several bacterial pigments that have potential as sunscreen agents because they have better survival with UV radiation (Patki et al, 2021). For instance, Suryawanshi et al (2015) stated that bacterial pigments such as violacein and prodigiosin can enhance the sun protection factor (SPF), which is a measurement of how well the UVB rays could be absorbed by a sunscreen product.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Used and Fresh Palm Oil on Prodigiosin Production for Potential Sunscreen Agent

Chong,

Norhesam,

Zulkharnain

et al. 2023

BBT

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Prodigiosin is a secondary metabolite red pigment and has unique triperrole structure and produced by gram-negative and gram-positive bacteria from gamma-proteobacteria and actinomycetes class. It is located in the within the cell membrane, extracellular vesicle, and intracellular granules, thus, it needs to be extracted to obtain prodigiosin. Prodigiosin has many properties such as antioxidant, anticancer, anti-microbial and anti-proliferative properties and it can be used in food, textile, and pharmaceutical industry as a natural colourant. Prodigiosin is produced through fermentation process Serratia nematodiphila. There was high demand in producing natural pigments due to toxicity of synthetic pigment and high-cost production of prodigiosin. The prodigiosin production were affected by many parameters such as concentration and type of substrates during fermentation. The substrates such as carbon and nitrogen sources are main substrates affected the prodigiosin production. This study aimed to enhance prodigiosin production by comparing the effect of used and fresh palm oil and prodigiosin as a potential sunscreen agent. The prodigiosin production was done through fermentation of Serratia nematodiphila in a shake flask and extracted by using acidified methanol. The effect of production of prodigiosin was determined at different concentration of different carbon sources which are used palm oil and fresh palm oil with constant value of pH, temperature, agitation speed to produced high yield of prodigiosin. In vitro sun protection factor (SPF) was used to determine the spectrophotometric SPF value of the prodigiosin. The highest yield of prodigiosin production with 8 % (w/v) of used palm oil was 463.938 µmol/L and with 9 % (w/v) of fresh palm oil was 518.84 µmol/L. The specific growth rate was 0.112 h-1 and doubling time was 6.18 h with 8% of used palm oil at incubation time of 46 h. While for 8 % fresh palm oil had specific growth rate of 0.104 and doubling time of 6.69 at incubation time for 40h. The SPF value with 1 % (w/v) of prodigiosin was 3.68.

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Section: Potential As Sunscreen Agentmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Effects of Used and Fresh Palm Oil on Prodigiosin Production for Potential Sunscreen Agent

Chong,

Norhesam,

Zulkharnain

et al. 2023

BBT

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“…P1 produces a yellow pigment tentatively identified as zeaxanthin. The pigment extract at 5 mg/mL showed a broad range of antibacterial activity via the disk diffusion test, with haloes around 15 mm against S. aureus and E. coli [45]. Chryseobacterium strains present biosynthetic gene clusters responsible for the production of antimicrobials and may produce antibiotics [46].…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Production, Extraction and Partial Characterization of Natural Pigments from Chryseobacterium sp. kr6 Growing on Feather Meal Biomass

Gemelli,

Silveira,

Pailliè-Jiménez

et al. 2024

Biomass

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Obtaining natural pigments from microorganisms is an alternative with high potential for biotechnological application. The use of agro-industrial wastes as substrate for cultivations enables a reduction of the production cost and may add value to potentially polluting byproducts. In this work, the extraction of pigments produced by the bacterium Chryseobacterium sp. strain kr6 was evaluated, employing feather meal as the sole carbon source for bacterial growth. The maximum production of the yellow pigments was observed for cultivation at 30 °C, during 48 h, with 5 g/L feather meal. The pigment extraction from the bacterial biomass was performed with the aid of physical methods and the testing of different organic solvents. The conditions that provided better extraction were using ultrasound with acetone as the solvent, reaching a yield of 180 μg/g biomass after optimization. The pigment was partially characterized via UV-visible, FTIR and mass spectroscopy and CIELAB color parameters, suggesting the presence of molecules belonging to the flexirubin group (aryl polyenes). The antioxidant capacity of the pigment was confirmed via the scavenging of DPPH radical and thiobarbituric acid reactive substances (TBARS) methodologies. Moreover, the pigment extract showed antimicrobial activity against Staphylococcus aureus and Enterococcus faecalis.

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“…Bacterial pigments like prodigiosin, violacein and melanin have been shown to be UV protective (Darshan & Manonmani, 2015; Kothari et al, 2017; Siezen, 2011; Patki et al, 2021). Many of them also have antimicrobial, antioxidant, anticancer, anti‐obesity and anti‐inflammatory properties which make them useful in the food, textiles, printing, pharmaceutical, and cosmetics industries (Revuelta & Vandamme, 2016; Sa'nchez‐Mun˜oz et al, 2020; Venil et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Identification of a novel quinoline-based UV-protective pigment from a psychrotrophic Arctic bacterium

Mandal

Kundu

Uddin

et al. 2022

Journal of Applied Microbiology

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Aims: Psychrotrophs are extremophilic microorganisms that grow optimally in low temperature having many unique bioactive molecules of biotechnological applications. In this study, we characterized a pigment from an arctic bacterium with protective activity towards UV exposure. Methods and results:The present research reports isolation and characterization of a psychrotrophic bacteria, RSAP2, from the soil sample of NyAlesund (78°56"N, 11°54"E), Svalbard, Norway. The strain showed closest 16S rRNA gene sequence similarity (99.9%) with Kocuria indica NIO-1021. RSAP2 is a Gram-positive, coccoid aerobe which produces a yellow pigment. The optimal parameters for pigment production while grown in LB medium were 3% (w/v) NaCl and 4 days of incubation of the culture at 20°C and pH 9 with shaking (180 rpm). The pigment was extracted in methanol and acetone (2:1) and further purified through column chromatography. It was characterized by mass spectrometry, UV-visible, fluorescence, IR, 1 H NMR, 13 C NMR spectroscopy and CHNS/O analysis. The pigment has a molecular weight of about 258 daltons and the molecular formula was determined as C 15 H 18 N 2 O 2 and is a quinoline derivative. We show that the pigment can protect Escherichia coli against UV-mediated mutagenesis. We further demonstrate that the pigment displays a significant antimicrobial effect and in sublethal concentrations it impairs biofilm formation ability of the model organism Staphylococcus aureus. Conclusions:The pigment of a psychrotrophic Arctic bacterium, most likely a strain of K. indica, was purified and its chemical structure was determined. The quinolinebased pigment has the ability to protect live cells from UV induced damage.Significance and impact of study: Analysis and characterization of this newly isolated quinoline-based pigment is a potential candidate for future application in skin care products.

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Analysis of the applicative potential of pigments extracted from bacterial isolates of mangrove soil as topical UV protectants

Cited by 6 publications

References 32 publications

Effects of Used and Fresh Palm Oil on Prodigiosin Production for Potential Sunscreen Agent

Effects of Used and Fresh Palm Oil on Prodigiosin Production for Potential Sunscreen Agent

Production, Extraction and Partial Characterization of Natural Pigments from Chryseobacterium sp. kr6 Growing on Feather Meal Biomass

Identification of a novel quinoline-based UV-protective pigment from a psychrotrophic Arctic bacterium

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