Anti-inflammatory activity and identification of the Verbena litoralis Kunth crude extract constituents

Abstract

Verbena litoralis is a plant popularly known as "gervãozinho-do-campo" in Portuguese. It is traditionally used for stomach, liver and gallbladder problems, and as an anti-inflammatory and anthelmintic. The goal of this study was to determine the chemical composition of the crude extract obtained from the aerial parts of V. litoralis by Ultra High Performance Liquid chromatography coupled with Mass Spectrometry in Tandem (UHPLC-MS/MS); assess the anti-inflammatory activity of ethyl acetate fraction and of crude extract; and verify liver, kidney and pancreas damage. In this study, the chemical composition of the extract was identified via UHPLC/MS/MS, assessing the anti-inflammatory activity of the crude extract and the acetate fraction in an induction model of the granulomatous tissue, as well as given liver, kidney and pancreas damage markers. Chlorogenic acid, luteolin, caffeic acid, apigenin, p-coumaric acid, vanillic acid, ferulic acid and quercetin were quantified in the extract. After the seven-day treatment, the granuloma of the animals treated with the plant extract and fraction presented values very close to the positive control (nimesulide). The V. litoralis crude extract and ethyl acetate fraction show antiinflammatory activity similar to the nimesulide without evidence of liver, kidney and pancreas damage, which attributes the plant's pharmacological action to the flavonoids found.

Anti-Inflammatory Effects of an Aqueous Extract of Justicia Flava (Forsk) Vahl (Acanthaceae) in Rats

“…Similar results were obtained by Marref et al [20] who found out that the methanolic extract of G. segetum did not significantly alter the number of blood platelets compared to control group in inflammation elicited by cotton pellet insertion in rats. Similarly Lima et al [31] showed that the crude extract of Verbena litoralis used in the cotton pellets test did not affect the platelet count compared to the negative control group.…”

Potential Therapeutic Implications of Caffeic Acid in Cancer Signaling: Past, Present, and Future

“…There are several beneficial effects of CA and its derivatives, including anti-bacterial ( Matejczyk et al, 2018 ; Mitani et al, 2018 ), anti-viral ( Langland et al, 2018 ; Shen et al, 2018 ), anti-oxidant ( Shiozawa et al, 2018 ; Kfoury et al, 2019 ), anti-inflammatory ( Zaitone et al, 2019 ; Lima et al, 2020 ), immune-stimulatory ( Krifa et al, 2013 ; Coleman et al, 2016 ), antidiabetic ( Chiou et al, 2017 ; Bounegru and Apetrei, 2020 ), cardioprotective ( Agunloye et al, 2019 ; Salau et al, 2021 ), anti-proliferative ( Pelinson et al, 2019 ), hepatoprotective ( Abdelhafez et al, 2018 ; Saleem et al, 2019 ), anti-cancer ( Zeng et al, 2018 ; Martini et al, 2019 ; Pelinson et al, 2019 ), and so on. CA plays a central function in the human system because of its many beneficial effects.…”

Voltamperometric Sensors and Biosensors Based on Carbon Nanomaterials Used for Detecting Caffeic Acid—A Review

“…CA participates in the defense mechanism of plants against predators, pests, and infections, because it has an inhibitory effect on the growth of fungi and bacteria [28,29]. In vitro and in vivo experiments have demonstrated that CA and its derivatives have numerous beneficial physiological effects, such as antibacterial [30][31][32], antiviral [33][34][35][36], antioxidant [37][38][39][40], anti-inflammatory [5,[41][42][43], anti-atherosclerotic [44][45][46], immunostimulatory [47][48][49], antidiabetic [50,51], cardioprotective [52,53], antiproliferative [54], hepatoprotective [55,56], anticancer [20,54,[57][58][59], etc.…”