Determination of phenolic and triterpenic compounds in Jatropha gossypiifolia L by Ultra-high performance liquid chromatography-tandem mass spectrometric (UHPLC-MS/MS)

Abstract

This work describes the chemical characterization of extracts of Jatropha gossypiifolia (from Amazonia region) concerning the presence of phenolic and triterpenic compounds using Ultra High Perfomance Liquid chromatography coupled to Mass Spectrometry in Tandem (UHPLC-MS/MS) methods. The studied compounds belong to the most frequently found classes in medicinal plants (triterpenes, flavonoids, flavones, hydroxycinnamic acids, coumarins, catechins and stilbenes), which have been analyzed as chemical and bioactive markers in hydroethanolic and aqueous extracts. Several polyphenolic compounds identified herein are unprecedented in the scientific literature for this species. The chemical markers identified and quantified in the studied extracts of J. gossypiifolia were gallic acid, chlorogenic acid, catechin, caffeic acid, vanillic acid, p-coumaric acid, ferullic acid, rutin, quercitrin, 3-acetylcoumarin, trans-cinnamic acid, quercetin, luteolin, apigenin, kaempferol, chrysin. α-amyrin, β-amyrin and lupeol. As the majority compounds, (+)-catechin, p-coumaric acid, ferulic acid, luteolin, α-amyrin and β-amyrin were found to be present at mg kg −1 levels. J. gossypiifolia extracts presented a high in vitro activity against different reactive oxygen species (hydroxyl, peroxyl, and superoxide anion radicals). Several polyphenolic compound data presented herein are unprecedented in the scientific literature for this plant species. As a result, this plant can be a new source of bioactive molecules for therapeutic purposes.

Determination of Chemical Constituents and Antioxidant Activities of Leaves and Stems from Jatropha cinerea (Ortega) Müll. Arg and Jatropha cordata (Ortega) Müll. Arg

“…The phenolic compounds present in the leaves and stems from J. cinerea and J. cordata are showed in Table 3. The phytochemical compounds profile identified in the alcoholic extracts of leaves and stems from J. cinerea and J. cordata is similar to that already reported for Jatropha gossypiifolia, being p-coumaric, ferulic, caffeic, gallic, vanillic, and gentisic acids, catequin, vitexin, isovitexin, luteolin and apigenin the main compounds [31,32]. Respect to flavonoids, J. cinerea and J. cordata presented quercetin and rhoifolin, which are the same compounds reported in Jatropha curcas L. [15].…”

HPLC/ESI-MS and NMR Analysis of Chemical Constitutes in Bioactive Extract from the Root Nodule of Vaccinium emarginatum

“…Fragmentation pattern of Peaks 9, 11, 15 and 16 displayed the diagnostic ions at m/z 301, suggesting that these compounds were quercetin and quercetin derivatives [20]. For the hyperin and isoquercitin, the spectra showed the ion related to the deprotonated aglycone [A − H] − , which is formed by loss of the galactose or glucose moiety from the flavonol glycosides [17] In addition, the peaks were identified as lupeol, β-amyrin, α-amyrin and friedelin according to the retention time, the literature and the isolated chemicals in this study [21][22][23].…”

“…Twenty-two peaks were identified as five flavonoids, three flavonol glycosides, six procyanidins, one phenolic acid, five triterpenoids and two unknown chemicals. LC-ESI-MS/MS with negative ion detection had been dramatically used for tentative identification of a variety of phenolic compounds in fruits [3,17,19], flowers [20], and traditional herb medicine [18,22]. LC-ESI-MS/MS is a great analytical tool because of its high selectivity for analyzing complex components in plants.…”

Assessment of Photoprotective, Antioxidant and Anti-Skin Cancer Activities of Leaf Extracts of Certain Medicinal Plants

“…Previous studies indicated the importance of antioxidant activity in UV protection. Furthermore, high SPF values were found to be correlated to high flavonoid and phenolic contents of plant extracts (Brewer, 2011;Ebrahimzadeh et al, 2014;Silveira et al, 2020). For many centuries, plants have been a rich source for therapeutic purposes.…”