Development and optimization of erythromycin-loaded lipid-based gel by Taguchi design: In vitro characterization and antimicrobial evaluation

Abstract

The foremost aim of the current research was to prolong and sustain the release of erythromycin (ERY) by preparing a solid lipid nanoparticles (SLNs)-based gel formulation for the safe and effective treatment of acne. ERY-loaded SLNs were developed, and various process variables were optimized with respect to particle size, zeta potential, and entrapment efficiency using the Taguchi model. The average particle size, PDI, zeta potential, drug entrapment efficiency, and drug loading of optimized SLN (F4) were found to be 176.2±1.82 nm, 0.275±0.011,-34.0±0.84, 73.56%, and 69.74% respectively. The optimized SLN (F4) was successfully incorporated into the carbopol-based hydrogel. The in vitro release of ERY from the SLN gel and plain gel were compared and found to be 90.94% and 87.94% respectively. In vitro study of ERY-loaded SLN gel showed sustained delivery of drug from formulation thus enhancing the antimicrobial activity after 30 hours when compared to ERY plain gel.

The Analysis of Chitosan-Coated Nanovesicles Containing Erythromycin—Characterization and Biocompatibility in Mice

“…In another study, in vitro characterization and antimicrobial evaluation were performed for ERT-loaded solid lipid nanoparticles gel compared to ERT plain gel. It was registered a sustained delivery of drug from the novel formulation enhancing the anti-infective activity after 30 h [ 43 ]. ERT and gentamicin were encapsulated into liposomes composed of 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine (0.11382 g/mL) and cholesterol (0.01 g/mL) at a molar ratio of 6:1 with increased activity on the Pseudomonas aeruginosa , diminishing the minimum inhibitory and bactericidal concentrations by 4–32 fold overall [ 44 ].…”

“…Furthermore, the gel-based lipid systems measuring 176.2 and 374 nm were obtained for the release of ERT with antimicrobial efficacy on Pseudomonas aeruginosa . [ 43 ]. Other researchers have used a dehydration-rehydration method to produce azithromycin-loaded liposomes based on dipalmitoyl-sn-glycero-3-phosphocholine and cholesterol, with an average diameter of 406.07 ± 45 nm [ 46 ].…”

Erythromycin Formulations—A Journey to Advanced Drug Delivery

“…A somehow similar study reported the encapsulation of ERY-SLNs (~176 nm) into a Carbopol gel [ 75 ]. The resulting products exhibited good properties for topical applications such as homogeneity, spreadability and extrudability.…”

“…Pursuing the preparation of a topical formulation aimed at the treatment of acne, Dhillon et al investigated the development and optimization of ERY-loaded SLNs. The study focused on selecting the best preparation method, lipid type, drug/lipid ratio, stirring, and sonication time [ 75 ]. The findings led to the conclusion that the microencapsulation technique was superior to the solvent evaporation and the solvent emulsification diffusion ones, because it showed better results in terms of particle size, particle shape and drug entrapment.…”