Simultaneous determination of anti-diabetic drugs

Sher, Nawab; Fatima, Nasreen; Perveen, Shahnaz; Siddiqui, Farhan Ahmed

doi:10.1590/s2175-97902019000217394

Cited by 7 publications

(6 citation statements)

References 25 publications

(18 reference statements)

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“…In the same way, absorbance of glimepiride also appeared at the maximum wavelength of metformin. Hence, the determination of the contents of the two compounds used simultaneous method [10,16,19].…”

Section: Resultsmentioning

confidence: 99%

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Simultaneous Determination of Metformin and Glimepiride in Fixed-Dose Combination Tablets Sold in Pramuka Market using Validated UV Spectrophotometric Method

Zilhadia¹,

Supandi

Alfarisi

2021

J Sains Farm Klin

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Combination of metformin and glimepiride as a diabetes mellitus drug has recently shown significant increase due to its benefits in improving patient compliance and offering cost effectiveness. This study aimed at analyzing metformin-glimepiride in fixed dose combinations purchased at Pramuka Market (the largest drug market in Jakarta) using validated simultaneous UVspectrophotometry. The maximum wavelength of metformin and glimepiride is 237 nm and 228 nm respectively. The validation result of metformin and glimepiride at concentration range of 2-8 µg/mL and 5-17 µg/mL showed Linearity with 0.9999 and 0.9996, LOD values with 0.0962 µg/mL and 0.2954 µg/mL, LOQ values with 0.3208 µg/mL and 0.9846 µg/mL, and recovery (accuracy test) with 99.72% ± 1.88% to 103.86 ± 0.87%, respectively. The Relative Standard Deviation (RSD) as the precision test indicator for both drugs was less than 2%. The level of Metformin-glimepiride fixed-dose combination purchased at Pramuka Market was about 96.15% ± 3.56% to 102.71% ± 1.50%. The conclusion of this study showed that the level of metformin-glimepiride sold in the Pramuka market met the requirements.

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Section: Resultsmentioning

confidence: 99%

“…The common methods used to analyze metformin and glimepiride are HPLC [10,11], LC-MS/MS [12], and UPLC-QToF-MS [13]. The principle of the three methods is separation based on polarity differences in 2 phases with high technology and higher cost [14].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Metformin and Glimepiride in Fixed-Dose Combination Tablets Sold in Pramuka Market using Validated UV Spectrophotometric Method

Zilhadia¹,

Supandi

Alfarisi

2021

J Sains Farm Klin

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“…The calibration curve was produced using a regression equation (y=c+mx). From this equation, intercept, slope, relation coefficient was subsequently calculated [19]. To specify the study, joint recipients used in ophthalmic drops were spiked with a pre-weighed quantity of drugs.…”

Section: Methods Validationmentioning

confidence: 99%

A Novel RP-HPLC method for simultaneous estimation of Dorzolamide and Timolol maleate in ophthalmic products

Sher¹,

Shafi²,

Naseem³

et al. 2021

PJC

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This research introduces an RP-HPLC method to estimate dorzolamide and timolol maleate quantitatively in the simultaneous mode. The stationary phase has consisted of A phenomenix® C18 column which gave satisfactory separation of these drugs when subjected to analysis, where in the mobile phase is composed of Acetonitrile:Buffer (20:80,v/v). The instrument was set up as such that the pump draws mobile phase at a flow rate of 1.0 mL/min and monitored at 290nm wavelength. Linear relationship of concentration of drugs vs. peak area was ascertained to assess linearity parameters and a correlation coefficient of more than 0.9995 for all the drugs was achieved when studied in a range of 10 to 1000 ug/mL. The newly developed method showed high accuracy and precision with a percent recovery of 98.85% -101.23% and a percent relief standard deviation of 0.5 -1.7 %. The optimized method was validated according to the guideline laid down by ICH and was successfully applied to quantitate both the drugs in bulk, raw material, eye drops formulation and in physiological fluids.

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“…Following a thorough study of the literature, several methods for analyzing MET alone in various samples (plasma, urine, liver, brain, kidney, and muscles) were reported, including HPLC [14][15][16][17][18][19], HILIC [20], LC/MS/MS [21][22][23][24], HILIC/MS [25], and GC/MS [26]. MET in conjunction with various medications in various plasma samples was also estimated by HPLC [27][28][29][30][31][32], LC/MS/MS [33][34][35][36][37][38][39][40], and HILIC/MS [41,42] methods.…”

Section: Introductionmentioning

confidence: 99%

Development and Greenness Assessment of HPLC Method for Studying the Pharmacokinetics of Co-Administered Metformin and Papaya Extract

et al. 2022

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Foods with medical value have been proven to be beneficial, and they are extensively employed since they integrate two essential elements: food and medication. Accordingly, diabetic patients can benefit from papaya because the fruit is low in sugar and high in antioxidants. An RP-HPLC method was designed for studying the pharmacokinetics of metformin (MET) when concurrently administered with papaya extract. A mobile phase of 0.5 mM of KH2PO4 solution and methanol (65:35, v/v), pH = 5 ± 0.2 using aqueous phosphoric acid and NaOH, and guaifenesin (GUF) were used as an internal standard. To perform non-compartmental pharmacokinetic analysis, the Pharmacokinetic program (PK Solver) was used. The method’s greenness was analyzed using two tools: the Analytical GREEnness calculator and the RGB additive color model. Taking papaya with MET improved the rate of absorption substantially (time for reaching maximum concentration (Tmax) significantly decreased by 75% while maximum plasma concentration (Cmax) increased by 7.33%). The extent of absorption reduced by 22.90%. Furthermore, the amount of medication distributed increased (30.83 L for MET concurrently used with papaya extract versus 24.25 L for MET used alone) and the clearance rate rose by roughly 13.50%. The results of the greenness assessment indicated that the method is environmentally friendly. Taking papaya with MET changed the pharmacokinetics of the drug dramatically. Hence, this combination will be particularly effective in maintaining quick blood glucose control.

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Simultaneous determination of anti-diabetic drugs

Cited by 7 publications

References 25 publications

Simultaneous Determination of Metformin and Glimepiride in Fixed-Dose Combination Tablets Sold in Pramuka Market using Validated UV Spectrophotometric Method

Simultaneous Determination of Metformin and Glimepiride in Fixed-Dose Combination Tablets Sold in Pramuka Market using Validated UV Spectrophotometric Method

A Novel RP-HPLC method for simultaneous estimation of Dorzolamide and Timolol maleate in ophthalmic products

Development and Greenness Assessment of HPLC Method for Studying the Pharmacokinetics of Co-Administered Metformin and Papaya Extract

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