Potency of nano-antibacterial formulation from Sargassum binderi against selected human pathogenic bacteria

Moni, Sivakumar Sivagurunathan; Alam, Mohammad Firoz; Safhi, Mohammed M.; Jabeen, Aamena; Sanobar, Syeda; Siddiqui, Rahimullah; Moochikkal, Remesh

doi:10.1590/s2175-97902018000417811

Cited by 18 publications

(9 citation statements)

References 14 publications

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“…Nanoparticle formulation is a very important technology that can be used to solve problems of drug resistance that arise in conventional antibiotic therapy 29 , 30 . The advantage of nanoparticles lies in their use for eliciting intracellular effects in specific cells.…”

Section: Resultsmentioning

confidence: 99%

Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent

Madkhali

Moni

Sultan

et al. 2021

Sci Rep

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The purpose of this study was to develop a novel nano antibacterial formulation of dextran sulfate sodium polymer. The dextran sulfate sodium (DSS) nanoparticles were formulated with gelation technique. The nanoparticles exhibited significant physicochemical and effective antibacterial properties, with zeta potential of − 35.2 mV, particle size of 69.3 z d nm, polydispersity index of 0.6, and percentage polydispersity of 77.8. The DSS nanoparticles were stable up to 102 °C. Differential scanning calorimetry revealed an endothermic peak at 165.77 °C in 12.46 min, while XRD analysis at 2θ depicted various peaks at 21.56°, 33.37°, 38.73°, 47.17°, 52.96°, and 58.42°, indicating discrete nanoparticle formation. Antibacterial studies showed that the DSS nanoparticles were effective against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentrations of DSS nanoparticles for Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S. pyogenes), Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Klebsiella pneumoniae (K. pneumoniae) and Proteus vulgaris (P. vulgaris) were 150, 200, 250, 150, 200, 250, 250 µg/mL, respectively. The antibacterial effects of DSS nanoparticles were in the order E. coli (26 ± 1.2 mm) at 150 µg/mL > S. pyogenes (24.6 ± 0.8 mm) at 250 µg/mL > B. subtilis (23.5 ± 2 mm) at 150 µg/mL > K. pneumoniae (22 ± 2 mm) at 250 µg/mL > P. aeruginosa (21.8 ± 1 mm) at 200 µg/mL > S. aureus (20.8 ± 1 mm) at 200 µg/mL > P. vulgaris (20.5 ± 0.9 mm) at 250 µg/mL. These results demonstrate the antibacterial potency of DSS injectable nanoparticles.

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Section: Resultsmentioning

confidence: 99%

Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent

Madkhali

Moni

Sultan

et al. 2021

Sci Rep

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“…A previous study reported that fucosterol isolated from S. binderi exerts anti-inflammatory activity in human lung epithelial cells [ 24 ]. Similarly, other S. binderi extracts showed antibacterial activity against human pathogenic bacteria [ 25 ], and toxicology studies based on these extracts showed that S. binderi could be used in the preparation of functional foods [ 26 ]. However structural and monosaccharide content characterization of fucoidans isolated from S. binderi remains unexplored [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Purification and Structural Characterization of Sulfated Polysaccharides Derived from Brown Algae, Sargassum binderi: Inhibitory Mechanism of iNOS and COX-2 Pathway Interaction

Lee

Fernando

et al. 2021

Antioxidants

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Among the components derived from brown algae, anionic sulfated polysaccharides, which contain sulfated fucose as the major monosaccharide, exert significant biological activities. In this study, we purified and structurally characterized sulfated polysaccharides from brown algae, Sargassum binderi (S. binderi; SBPs), and evaluated their biological activity in vitro and in vivo. The SBPs were separated based on their charges and their biophysical properties were investigated according to their functional groups, structural features, and molecular weights using FTIR, NMR, and MALS. Among all the SBPs, Fraction 4 (SBP-F4), with an average molecular weight of 2.867 × 105 g/mol, had the highest polysaccharide and sulfate contents (75.15 ± 0.25% and 24.08 ± 0.18%, respectively). The biological activities of SBP-F4 were investigated further in vitro and in vivo. Our results showed that SBP-F4 significantly suppressed the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins in LPS-activated macrophages. Moreover, in the LPS-treated zebrafish model, a significant decrease in cell death and NO production was observed. Collectively, these results show that SBPs not only exert protective effects against LPS-induced cytotoxicity but also inhibit the activation and anti-inflammatory activity of macrophages. Therefore, polysaccharides derived from S. binderi are potential anti-inflammatory agents for use in clinical settings.

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“…Recently, a study reported that the methanolic extract of S. tenerrimum contains steroids, saponins, alkaloids, tannins, flavonoids, terpenoids proteins, and phenolic compounds (Kumar et al, 2012). In continuation of our earlier works (Moni et al, 2019;Moni et al, 2018), the present study was designed to characterize the bioactive principles found in S. tenerrimum using gas chromatography-mass spectrometry (GC-MS)…”

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confidence: 91%

Spectral analysis and Antibacterial activity of the bioactive principles of Sargassum tenerrimum J. Agardh collected from the Red sea, Jazan, Kingdom of Saudi Arabia

Albratty

Alhazmi

Meraya³

et al. 2023

Braz. J. Biol.

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Seaweeds are a major marine resource that can be explored to develop novel pharmaceutical molecules. The present study showed the presence of unique bioactive components in the petroleum ether extract (PEE) and methanolic extract (ME) of Sargassum tenerrimum. The gas chromatography-mass spectrometry analysis suggested that the PEE of S. tenerrimum contained antibacterial biomolecules: hexadecanoic acid, methyl ester, 17-pentatriacontene, dasycarpidan-1-methanol, and acetate (ester). However, the ME of S. tenerrimum exhibited better antibacterial effect than the PEE due to the presence of the bioactive compounds 1,2-benzenedicarboxylic acid, diisooctyl ester, tetratetracontane, 1-docosene, 1,2-benzenediol, and benzoic acid. Thus, promising antibacterial molecules can be isolated from S. tenerrimum for better therapeutic use.

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Potency of nano-antibacterial formulation from Sargassum binderi against selected human pathogenic bacteria

Cited by 18 publications

References 14 publications

Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent

Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent

Purification and Structural Characterization of Sulfated Polysaccharides Derived from Brown Algae, Sargassum binderi: Inhibitory Mechanism of iNOS and COX-2 Pathway Interaction

Spectral analysis and Antibacterial activity of the bioactive principles of Sargassum tenerrimum J. Agardh collected from the Red sea, Jazan, Kingdom of Saudi Arabia

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