Comparative evaluation of pancreatic histopathology of rats treated with olanzapine, risperidone and streptozocin

Abstract

Olanzapine and risperidone are widely prescribed atypical antipsychotics used in the treatment of schizophrenia and various other psychiatric disorders. Both of these drugs have been extensively reported to cause Type 2 diabetes mellitus and pancreatitis, however, the mechanism of olanzapine and risperidone-induced toxicity has not been so far unveiled. We, therefore, compared the streptozocininduced pancreatic damage with that of pancreas isolated from olanzapine and risperidone treated rats. It was noticed that fibrotic growth, necrosis and derangement of the pancreatic islet cells caused by streptozocin were more pronounced than olanzapine and risperidone.

Multi-strain probiotic supplement attenuates streptozotocin-induced type-2 diabetes by reducing inflammation and β-cell death in rats

“…In order to mitigate toxicity severity and cell mortality (but the beta cells weren't completely damaged), we implemented 10-20mg/kg dosage in every two days for 8 days. This animal model was optimized according to previous PLOS ONE study, in which multiple low doses of STZ and high-fat diet (HFD) was followed to induce T2DM [30,31]. Therefore, this optimized method was primarily designed to induce slow and stable damage of beta cells to generate T2DM model rats(S2 Table in S1 Text).…”

“…Streptozotocin (STZ) is commonly used for targeting insulin producing beta cells in pancreas to induce hyperglycemia and mimic T2DM disease conditions. Conventionally, a dosage of 50mg/kg is used [ 30 ]. Since STZ is toxic to beta cells, this dosage has been shown to result in necrosis and undesirable high beta cell mortality [ 30 ].…”

“…Conventionally, a dosage of 50mg/kg is used [ 30 ]. Since STZ is toxic to beta cells, this dosage has been shown to result in necrosis and undesirable high beta cell mortality [ 30 ]. In order to mitigate toxicity severity and cell mortality (but the beta cells weren’t completely damaged), we implemented 10-20mg/kg dosage in every two days for 8 days.…”

Endangered Lymphocytes: The Effects of Alloxan and Streptozotocin on Immune Cells in Type 1 Induced Diabetes

“…Samples were fixed in a solution of 10% paraformaldehyde, dehydrated with alcohol, diaphanized in xylol, and embedded in paraffin. Blocks were cut into 3 μm width slices, and fragments were captured with glass slides and stained with hematoxylin and eosin (H&E) [23]. General toxicity processes were described and classified according to structural changes by a semiquantitative analysis, as follows: absent, when there was no compromise of the tissue, score = 0; discrete, with up to 25% of area commitment, score = 1; moderate, from 26% to 50% of area commitment, score = 2; and accentuated, with more than 50% of area commitment, score = 3 [23][24][25].…”

Hydrogen-Rich Water Ameliorates Pancreatic Cell Necrosis and Regulates Cytokine Levels in Streptozotocin-Induced Diabetic Rats

“…The pancreatic histology were analyzed descriptively to determine changes in cell architecture, including fibrosis, necrosis, and vacuolization in the Langerhan islet. 27 Measurement of IL-10 and IL-1β concentrations in serum and liver tissues Blood samples were collected in a centrifuged tube from the heart after the rats were fasted overnight and allowed to stand for one hour at room temperature. The blood was then centrifuged at 3000 rpm for 20 min to separate the serum, the supernatant was then collected and stored at -20 o C before use.…”