Antibacterial and antibiofilm activity of carvacrol against Salmonella enterica serotype Typhimurium

Trevisan, Daliah Alves Coelho; Silva, Alex Fiori da; Negri, Melyssa; Filho, Benício Alves de Abreu; Machinski, Miguel; Patussi, Eliana Valéria; Campanerut‐Sá, Paula Aline Zanetti; Mikcha, Jane Martha Graton

doi:10.1590/s2175-97902018000117229

Cited by 36 publications

(20 citation statements)

References 35 publications

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“…Burt et al (2014), using other approaches, reported that carvacrol inhibited S. Typhimurium biofilm formation, probably by disrupting quorum sensing signaling. Similar to findings by Burt et al (2014), our previous study also showed the ability of carvacrol to decrease S. Typhimurium biofilm (Trevisan et al, 2018), corroborating with the hypothesis that these results may be a consequence of reduction of relative quantity of luxS.…”

Section: Discussionsupporting

confidence: 89%

“…Carvacrol (624 µg/ml) was able to reduce bacterial counts from 8.1 log 10 CFU/cm 2 to 4.1 log 10 CFU/cm 2 on S. Typhimurium biofilms. In a previous study, Trevisan et al (2018) also observed that carvacrol at 624 µg/ml was able to reduce about 50% of S. Typhimurium biofilm in polypropylene. Based on these results, this concentration was used to evaluate the protein changes in the present study.…”

Section: Proteins Changes In S Typhimurium Biofilm Exposed To Carvacrolmentioning

confidence: 75%

“…Recently, the effectiveness of carvacrol against bacterial biofilms has been investigated including those formed by Salmonella spp. (Amaral et al, 2015;Burt et al, 2014;Soni et al, 2013;Trevisan et al, 2018). Nevertheless, the mechanism of action of carvacrol against biofilms has not been completely elucidated.…”

Section: Discussionmentioning

confidence: 99%

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Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study

Trevisan¹,

Campanerut‐Sá²,

Silva³

et al. 2020

JAB

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Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In the present study, the aim was to evaluate protein profile changes in S. Typhimurium biofilm treated with carvacrol. Proteomic analysis of treated versus untreated biofilm showed several changes in proteins involved with S. Typhimurium biofilm and antioxidant activity. The proteins DsbA (thiol: disulfide interchange protein DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), and SODs (superoxide dismutases) A, B and C had their synthesis decreased after treatment with carvacrol. These proteins play a key role in S. Typhimurium biofilm formation, demonstrating the dynamic antibiofilm action of carvacrol. The differentially expressed proteins identified provide possible action targets for future studies in order to gain more insight into the mechanism of action of carvacrol on S. Typhimurium biofilm.

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Section: Discussionsupporting

confidence: 89%

Section: Proteins Changes In S Typhimurium Biofilm Exposed To Carvacrolmentioning

confidence: 75%

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Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study

Trevisan¹,

Campanerut‐Sá²,

Silva³

et al. 2020

JAB

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“…In order to investigate the morphological changes in both planktonic cells and biofilms after photoinactivation, scanning electron microscopy (SEM) was applied according to a procedure previously described . The concentration of PS and the time of light exposure employed were those that reduced ~50% in CFU levels (Figs.…”

Section: Methodsmentioning

confidence: 99%

Xanthene Dyes and Green LED for the Inactivation of Foodborne Pathogens in Planktonic and Biofilm States

Silva

Santos

Trevisan

et al. 2019

Photochem & Photobiology

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This study evaluated the rose bengal‐ and erythrosine‐mediated photoinactivation against Salmonella Typhimurium and Staphylococcus aureus planktonic and sessile cells using green LED as a light source. The free‐living or 2‐day‐old biofilm cells were treated with different concentrations of the photosensitizing agents and subjected to irradiation. Only 5 min photosensitization with rose bengal at 25 nmol L−1 and 75 μmol L−1 completely eliminated S. aureus and S. Typhimurium planktonic cells, respectively. Erythrosine at 500 nmol L−1 and 5 min of light exposure also reduced S. aureus planktonic cells to undetectable levels. Eradication of S. aureus biofilms was achieved when 500 μmol L−1 of erythrosine or 250 μmol L−1 of rose bengal was combined with 30 min of irradiation. Scanning electron microscopy allowed the observation of morphological changes in planktonic cells and disruption of the biofilm architecture after photodynamic treatment. The overall data demonstrate that rose bengal and erythrosine activated by green LED may be a targeted strategy for controlling foodborne pathogens in both planktonic and sessile states.

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“…All the aforementioned phytochemicals are classified as generally recognized as safe by the United States Food and Drug Administration (21 Code of Federal Regulation part 172.515) (Adams et al, 2004, 2005; Knowles et al, 2005). Previous studies have demonstrated the antibiofilm effect of TC, EG and CR in various bacteria such as Salmonella Typhimurium (Trevisan et al, 2018), S . Enteritidis (Čabarkapa et al, 2019), Listeria monocytogenes (Upadhyay et al, 2013) and Pseudomonas aeruginosa (Letsididi et al, 2018; Lou et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Trans-Cinnamaldehyde, Eugenol and Carvacrol Reduce Campylobacter jejuni Biofilms and Modulate Expression of Select Genes and Proteins

et al. 2019

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Campylobacter jejuni is the leading cause of human foodborne illness globally, and is strongly linked with the consumption of contaminated poultry products. Several studies have shown that C. jejuni can form sanitizer tolerant biofilm leading to product contamination, however, limited research has been conducted to develop effective control strategies against C. jejuni biofilms. This study investigated the efficacy of three generally recognized as safe status phytochemicals namely, trans -cinnamaldehyde (TC), eugenol (EG), or carvacrol (CR) in inhibiting C. jejuni biofilm formation and inactivating mature biofilm on common food contact surfaces at 20 and 37°C. In addition, the effect of phytochemicals on biofilm architecture and expression of genes and proteins essential for biofilm formation was evaluated. For the inhibition study, C. jejuni was allowed to form biofilms either in the presence or absence of sub-inhibitory concentrations of TC (0.75 mM), EG (0.61 mM), or CR (0.13 mM) for 48 h and the biofilm formation was quantified at 24-h interval. For the inactivation study, C. jejuni biofilms developed at 20 or 37°C for 48 h were exposed to the phytochemicals for 1, 5, or 10 min and surviving C. jejuni in the biofilm were enumerated. All phytochemicals reduced C. jejuni biofilm formation as well as inactivated mature biofilm on polystyrene and steel surface at both temperatures ( P < 0.05). The highest dose of TC (75.64 mM), EG (60.9 mM) and CR (66.56 mM) inactivated (>7 log reduction) biofilm developed on steel (20°C) within 5 min. The genes encoding for motility systems ( flaA , flaB , and flgA ) were downregulated by all phytochemicals ( P < 0.05). The expression of stress response ( cosR , ahpC ) and cell surface modifying genes ( waaF ) was reduced by EG. LC-MS/MS based proteomic analysis revealed that TC, EG, and CR significantly downregulated the expression of NapA protein required for oxidative stress response. The expression of chaperone protein DnaK and bacterioferritin required for biofilm formation was reduced by TC and CR. Scanning electron microscopy revealed disruption of biofilm architecture and loss of extracellular polymeric substances after treatment. Results suggest that TC, EG, and CR could be used as a natural disinfectant for controlling C. jejuni biofilms in processing areas.

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Antibacterial and antibiofilm activity of carvacrol against Salmonella enterica serotype Typhimurium

Cited by 36 publications

References 35 publications

Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study

Action of carvacrol in Salmonella Typhimurium biofilm: A proteomic study

Xanthene Dyes and Green LED for the Inactivation of Foodborne Pathogens in Planktonic and Biofilm States

Trans-Cinnamaldehyde, Eugenol and Carvacrol Reduce Campylobacter jejuni Biofilms and Modulate Expression of Select Genes and Proteins

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