Hydroethanolic Allium sativum extract accelerates excision wound healing: evidence for roles of mast-cell infiltration and intracytoplasmic carbohydrate ratio

Abstract

The present study was designed to evaluate the in vivo effect of Allium sativum (garlic) hydroalcoholic extract on wound healing in rats. For this purpose, 72 mature Wistar rats were divided into four groups (n=18/each) to receive no treatment, placebo, Cicalfate®, or 2% Allium sativum (AS) extract, administered topically to the wound area, for 21 days. Following the experimental period, tissue samples were dissected out and underwent to histopathological analyses. Fibroblasts, fibrocytes, mast cells, intra-cytoplasmic carbohydrate ratio, neovascularization, collagen deposition, and re-epithelialization were analyzed in all groups. Animals in the treated groups showed significant enhancement in fibroblast, fibrocyte, and mast-cell distribution. Significantly higher neovascularization was observed on day 3 after wound induction in AS-treated animals versus those in the placebo, Cicalfate, and untreated groups (P<0.05). A dose-dependent, significantly higher intra-cytoplasmic carbohydrate storage was observed in treated animals. Our data show that AS promotes wound healing due to its preliminary impact on mast-cell distribution, which enhanced collagen synthesis and upregulated angiogenesis, and shortened the healing process by enhancing the intra-cytoplasmic carbohydrate ratio.Uniterms: Allium sativum/effects/rats. Allium sativum/wound healing/study. Allium sativum/mast cells. Allium sativum/angiogenesis. Intra-cytoplasmic carbohydrate.

Topical administration of hydroethanolic extract of Lawsonia inermis (henna) accelerates excisional wound healing process by reducing tissue inflammation and amplifying glucose uptake

“…Administration of L. inermis seems to accelerate epithelialization by provoking carbohydrates, especially glucose, reduce the distance between the wound edges and promote re‐epithelialization to shorten the necessary time for wound closure. In line with the present findings, our previous study showed that the hydroethanolic Allium sativum extract shorten the healing process by increasing the intracytoplasmic carbohydrate ratio . Glucose has been known to have important role in supporting the energy source for keratinocyte migration and proliferation by glycolysis pathway .…”

“…In line with the present findings, our previous study showed that the hydroethanolic Allium sativum extract shorten the healing process by increasing the intracytoplasmic carbohydrate ratio. 23 Glucose has been known to have important role in supporting the energy source for keratinocyte migration and proliferation by glycolysis pathway. 4 Our microscopic PAS analyses showed that administration of 3% and 6% L. inermis increased the intracytoplasmic carbohydrate ratio compared to the control animals demonstrating increased glucose uptake in the L. inermis-treated groups.…”

Topical application of Cinnamon verum essential oil accelerates infected wound healing process by increasing tissue antioxidant capacity and keratin biosynthesis

“…Thereafter, the photomicrographs were analyzed by Image Pro‐insight software (Version:9.00) for pixel‐based colorimetric intensity of collagen. The epithelial thickness was estimated by a morphometric lens (Olympus, Germany) and reported as micrometer …”

Medicinal Plants in Wound Healing

“…Farahpour et al [55] have shown that topical administration of Allium sativum accelerated wound healing because of its preliminary impact on mast-cell distribution and increased collagen synthesis and up-regulated angiogenesis, and improved the healing process by increasing the intra-cytoplasmic carbohydrate ratio.…”