Pulmonary effects of ozone therapy at different doses combined with antibioticotherapy in experimental sepsis model

Abstract

This experimental sepsis model created with Escherichia coli aimed to investigate the histopathological effects of two different doses of ozone combined with antibiotherapy on lung tissue. Methods: Rats were divided into 5 groups. Then sepsis was induced intraperitoneally in the first 4 groups. The 1 st group was treated with cefepime, the 2 nd and 3 rd groups were treated with cefepime combined with ozone at a dose of 0.6 mg/kg and 1.1 mg/kg. Lung tissue sections were stained with hematoxylin-eosin and assessed under light microscope and scored between 0-4 in terms of histopathological findings. Results: In the comparisons between Group 1 and Group 4 in terms of cellular damage (p=0.030), inflammation (p=0.000) and overall score (p=0.007), statistically significant positive effects were observed in favor of Group 1. In the comparisons of Groups 2 and 3 with Group 4, only positive effects were observed in terms of inflammation (p=0.020, p=0.012, respectively). Conclusion: Although negative histopathological effects of ozone on tissue injury were detected, it was noteworthy that the increase in the ozone dose reduced the number of damaged parameters.

A Systematic Biomedical Perspective on COVID-19 Pandemic: From Detection to Vaccination

“…Also, some experimental studies invoking the in vivo tests of coronavirus infected patients using OT have found this therapy beneficial in improving lung capacity, normalizing the redox potential, and also the activation of heme oxygenase (HO-1) [76]. Similar observations of the beneficial role of OT have been reported in recent case reports where O 3 has been found a potent anti-inflammatory tool [77]. Though some health supplements are also necessary addition for the COVID-19 treatment [78], the powerful antioxidant potential of O 3 has been suggested the key factor to increase O 2 in the blood and other tissues, showing profound implications in glycolysis, Krebs cycle, subcellular role in the mitochondrial antioxidative pathway and anti-inflammatory response in cytokines [79].…”

Lung Organoids—The Ultimate Tool to Dissect Pulmonary Diseases?

“…Respiratory-infection-linked sepsis deaths totaled 1.8 million globally in 2017 ( Rudd et al, 2020 ) driving urgent research into the condition. Sepsis has been experimentally induced in rodents by the administration of microbial components or living pathogens (e.g., Escherichia coli , or S. aureus ) into the bloodstream, peritoneal cavity, or trachea ( Karzai et al, 2003 ; Wang et al, 2004 ; Charavaryamath et al, 2006 ; Stahl et al, 2013 ; Kapicibasi et al, 2020 ). These models have shown that sepsis induced in mice by injection of peptidoglycan causes organ injury, organ, and systemic inflammation ( Wang et al, 2004 ).…”

Potential therapeutic effect of oxygen-ozone in controlling of COVID-19 disease

“…In an investigational sample of necrotizing pancreatitis, OT was found to be more operative in reducing oxidative stress, tissue damage, and bacterial translocation than hyperbaric oxygen therapy. 27 After using intraperitoneal ozone, Schulz et al 28 observed a decline in polymicrobial peritonitis.…”