Muscle changes with high-intensity aerobic training in an animal model of renal disease

Abstract

Purpose: To analyze the muscle changes with high-intensity aerobic training (HIAT) in an animal model of renal disease (RD). Methods: Twenty one adult Wistar rats were divided into 3 groups: healthy sedentary (HS), RD sedentary (RDS), RD aerobic training (RDAT). RDS and RDAT were subjected to unilateral renal ischemia-reperfusion (10 min) and 21days after that, RDAT was subjected to 6 weeks HIAT (swimming). Serum creatinine (Cr) and muscle morphometry (cross-sectional area = CSA) of gastrocnemius were analyzed. Results: Cr was higher (p = 0.0053) in RDS (0.82 ± 0.04) than in the others (RDAT 0.55 ± 0.04; HS 0.55 ± 0.04). Morphometric analysis (class interval of CSA in μm 2 /absolute frequency of muscle fibers in each class) indicated that 50th percentile occurred in: HS 7th class (3000.00-3499.00/515), RDS, 8th class (3500.00-3999.00/484), RDAT 5th class (2000.00-2499.00/856). CSA of largest fibers in RDS, RDAT, HS was 9953.00 μm 2 , 9969.00 μm 2 ,11228.00 μm 2 , respectively. High frequency of fibers with lower CSA occurred in 4th, 5th, 6th and 7th class in RDA, absence of fibers into 22nd, 23rd classes (RDS and RDAT). Conclusion: HIAT in an animal model of RD resulted in increased the number of muscle fibers with smaller CSA.

Effect of electrophysical resources on healing of neurotendinous injury in an experimental model of type I diabetes and kidney disease

“…The feasibility of using mice is due to the feasibility of producing their biological characteristics, in addition to having greater sensitivity to the use of streptozotocin. Previous studies allowed the effective induction of the kidney disease animal model in other animal species of the rodent class [11][12][13] . Patterns between groups were observed and described in relation to the organization of collagen fibers, presence of cellular tissue, inflammatory infiltrate, and signs of degeneration.…”

“…The feasibility of using mice is due to the feasibility of producing their biological characteristics, in addition to having greater sensitivity to the use of streptozotocin. Previous studies allowed the effective induction of the kidney disease animal model in other animal species of the rodent class 11 - 13 .…”

“…• Renal ischemia and reperfusion, by aseptic surgery [11][12][13] ; • Kidney damage was monitored for 14 days, using urine dipstick tests, in which renal damage was verified by the parameters pH, density, urobilinogen, and urinary glucose, as well as the presence of proteinuria and leukocytes. Renal damage was monitored for 14 days, using a urine reagent strip, by semiquantitative determination of 10 parameters by chemical reaction, in which the kidney was selected by the parameters pH, density, urobilinogen, and urinary glucose, in addition to the presence of proteinuria and leukocytes; • After 14 days of surgery for induction of kidney disease, the animals comprising the diseased groups were injected intraperitoneally (i.p.)…”

Treadmill Exercise Training Ameliorates Functional and Structural Age-Associated Kidney Changes in Male Albino Rats

“…One potential explanation for the insignificant difference in creatinine and creatinine clearance between the aged-S and aged-Ex groups may be the probability of increased muscle mass in the exercised group and the worse kidney function in the sedentary rats. The significant reduction in body weight of aged-Ex rats compared with aged-S rats could be attributed to the improved renal function with exercise training and subsequently decreased fluid retention [ 34 ].…”

Efeitos do exercício físico na prevenção e tratamento de lesões por isquemia: uma revisão de literatura

“…It was found, in one experiment, that 10 weeks of aerobic training and laryngine supplementation promote arteriogenesis of the cardiac and gastrocnemius muscles in parallel to the overexpression of TGF-β and negative regulation of angiostatin in rats with myocardial infarction 58 . Other results indicate that aerobic training protects the kidneys in ischemia and reperfusion injuries, and does not reverse the atrophy of muscle fibers with a greater transverse area, but increased the number of fibers with a smaller tanversal area 59 . The combination of early therapy with mesenchymal stem cells and resistance exercises does not enhance the benefits of such treatments for structural and functional cardiac remodeling in infarcted rats 60 .…”